Research

Tribulus Terrestris

97 peer-reviewed studies curated from PubMed and Semantic Scholar.

3
Meta-analyses
5
Systematic reviews
23
RCTs
66
Other studies
Meta-analyses (3%)
Systematic reviews (5%)
RCTs (24%)
Observational (2%)

Studies

Sorted by quality and recency

2025·Nutrients·José de Oliveira Vilar Neto, W. M. D. de Moraes, Daniel Vieira Pinto, et al

Effects of Tribulus (Tribulus terrestris L.) Supplementation on Erectile Dysfunction and Testosterone Levels in Men—A Systematic Review of Clinical Trials

Systematic reviewn = 483Sexual Health Mens Health

Systematic review of 10 studies evaluating Tribulus terrestris supplementation on erectile dysfunction and testosterone levels in men. TT improved erectile dysfunction in 3 of 5 studies, but only 2 studies showed a significant increase in testosterone levels, with low clinical magnitude.

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2025·International journal of impotence research·Sally Suharyani, Bella Amanda, Jesselyn Angellee, et al

Tribulus terrestris for management of patients with erectile dysfunction: a systematic review and meta-analysis of randomized trials.

Meta-analysisSexual Health

Systematic review and meta-analysis of randomized trials assessing Tribulus terrestris for erectile dysfunction. Tribulus terrestris improved IIEF-5 and IIEF-15 scores compared to placebo, with no significant difference in testosterone levels or adverse events.

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2023·Andrologia·Anam Ara, Rahul Vishvkarma, Poonam Mehta, et al

The Profertility and Aphrodisiac Activities of Tribulus terrestris L.: Evidence from Meta-Analyses

Meta-analysisn = 133Sexual Health Mens Health

Meta-analyses of 30 studies on Tribulus terrestris L. evaluating its profertility and aphrodisiac effects. In humans, TT significantly improved sperm concentration and motility. In rodents, TT improved sperm count, motility, and viability, with significant aphrodisiac activity observed in animal models.

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2021·Phytomedicine : international journal of phytotherapy and phytopharmacology·Nahdiya Sha'ari, Luke Sy-Cherng Woon, Hatta Sidi, et al

Beneficial effects of natural products on female sexual dysfunction: A systematic review and meta-analysis.

Meta-analysisSexual Health

Systematic review and meta-analysis of natural products for female sexual dysfunction (FSD). Tribulus terrestris showed significant positive effects on overall female sexual function, arousal, desire, and orgasm. Panax ginseng was effective in treating sexual arousal and desire. Other natural products like Trifolium pretense did not show significant effects.

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2020·Revista brasileira de ginecologia e obstetricia : revista da Federacao Brasileira das Sociedades de Ginecologia e Obstetricia·Ana Luiza Cabrera Martimbianco, Rafael Leite Pacheco, Fábia Lima Vilarino, et al

Tribulus Terrestris for Female Sexual Dysfunction: A Systematic Review.

Systematic reviewn = 279Sexual Health

Systematic review of 5 RCTs with 279 participants assessing Tribulus Terrestris for female sexual dysfunction. Treatment showed a significant increase in sexual function scores and serum testosterone levels in premenopausal women after 1 to 3 months. No serious adverse events reported, but the certainty of evidence is very low.

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2021·Journal of sex & marital therapy·Fabiene Bernardes Castro Vale, Junia Duelli Boroni, Guilherme Geber, et al

Effect of Tribulus Terrestris in the Treatment of Female Sexual Dysfunction and Clitoral Vascularization. Results of a Randomized Study Comparing Two Different Dosage Regimes.

RCTn = 104Sexual Health

RCT evaluating the efficacy of Tribulus Terrestris in two dosage regimes for treating sexual dysfunction in pre and postmenopausal women. Both dosage groups showed improvement in FSFI and QS-F scores, with no significant side effects. Clitoral artery PI showed no difference. Tribulus Terrestris increased total, free, and bioavailable testosterone.

PubMedRead on PubMed
2019·Revista Fitos·Amanda Carvalho De Sousa, M. A. Lima

Tribulus terrestris Linn as treatment of menopause symptoms: a systematic review

Systematic reviewWomens Health Sexual Health

Systematic review analyzing the effects of Tribulus terrestris on menopause symptoms. Studies found Tribulus to be better than placebo in reducing somatic, psychological, and urogenital symptoms, with improvements in vaginal lubrication and sensation during intercourse. It also increased free and bioavailable testosterone.

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2018·Complementary therapies in medicine·Sima Sanagoo, Behnaz Sadeghzadeh Oskouei, Nafiseh Gassab Abdollahi, et al

Effect of Tribulus terrestris L. on sperm parameters in men with idiopathic infertility: A systematic review.

Systematic reviewSexual Health

Systematic review of clinical trials and quasi-experimental studies on the effect of Tribulus terrestris L. on sperm parameters in idiopathic male infertility. The review included 7 articles, with 6 reporting improvements in sperm parameters such as number, motility, and morphology.

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2014·Journal of dietary supplements·Ahmed Qureshi, Declan P Naughton, Andrea Petroczi

A systematic review on the herbal extract Tribulus terrestris and the roots of its putative aphrodisiac and performance enhancing effect.

Systematic reviewSexual Health -Physical Performance -

Systematic review of Tribulus terrestris (TT) assessing its effect on testosterone levels in humans and animals. Limited evidence suggests TT is ineffective for increasing testosterone levels in humans, though some animal studies showed increased serum testosterone. Marketing claims of TT's effectiveness are unsubstantiated.

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2026·African journal of reproductive health·Uki R Budihastuti, Bhisma Murti, Teguh Prakosa, et al

Effectiveness of electroacupuncture in improving sperm motility and morphology in men with varicocele.

RCTn = 14Sexual Health

A single-blind, randomized controlled trial with 14 participants evaluated the effect of electroacupuncture combined with a herbal regimen and Tribulus Terrestris on sperm motility and morphology in men with varicocele. The EA group showed significant improvements in sperm morphology and motility compared to the control group.

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2018·Urologiia (Moscow, Russia : 1999)·L G Spivak, D V Platonova, D V Enikeev, et al

[Results of a comparative multi-center randomized clinical study of efficacy and safety of EFFEX Tribulus and Tribestan in patients with erectile dysfunction].

RCTn = 173Sexual Health Heart Health

RCT comparing the efficacy and safety of two herbal preparations, EFFEX Tribulus and Tribestan, based on Tribulus Terrestris in 173 patients with erectile dysfunction. Both groups showed significant improvements in erectile function and other related scores, with a decrease in serum cholesterol levels.

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2018·Journal of obstetrics and gynaecology : the journal of the Institute of Obstetrics and Gynaecology·Nasrin Baery, Azizeh Ghasemi Nejad, Mohsen Amin, et al

Effect of vaginal suppository on bacterial vaginitis based on Persian medicine (Iranian traditional medicine): a randomised double blind clinical study.

RCTn = 127Womens Health

This randomized double-blind clinical study compared the effects of a herbal vaginal suppository, Forzejeh, with metronidazole in 127 women with bacterial vaginosis. Both treatments significantly reduced symptoms and laboratory markers of bacterial vaginosis, with no significant difference between the two groups.

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2018·Urologia·Sameh Fayek GamalEl Din, Mohamed Ahmed Abdel Salam, Mohamed Shokr Mohamed, et al

Tribulus terrestris versus placebo in the treatment of erectile dysfunction and lower urinary tract symptoms in patients with late-onset hypogonadism: A placebo-controlled study.

RCTn = 70Sexual Health Mens Health

This placebo-controlled study evaluated the efficacy and safety of Tribulus terrestris in 70 aging males with erectile dysfunction and lower urinary tract symptoms. Tribulus terrestris significantly elevated total testosterone levels and improved erectile function scores without worsening lower urinary tract symptoms.

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2017·Maturitas·Zdravko Kamenov, Svetlana Fileva, Krassimir Kalinov, et al

Evaluation of the efficacy and safety of Tribulus terrestris in male sexual dysfunction-A prospective, randomized, double-blind, placebo-controlled clinical trial.

RCTn = 180Sexual Health

A Phase IV, randomized, double-blind, placebo-controlled clinical trial evaluated the efficacy and safety of Tribulus terrestris (Tribestan) in 180 men with erectile dysfunction (ED) and hypoactive sexual desire disorder (HSDD). The study found significant improvements in sexual function, including IIEF scores, intercourse satisfaction, orgasmic function, sexual desire, and overall satisfaction in the TT group compared to placebo. The therapy was well tolerated with no significant differences in adverse events.

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2017·Przegla̜d Menopauzalny = Menopause Review·S. Taavoni, N. Ekbatani, H. Haghani

Effect of Tribulus terrestris, ginger, saffron, and Cinnamomum on menopausal symptoms: a randomised, placebo-controlled clinical trial

RCTn = 80Womens Health

RCT of 80 postmenopausal women aged 50-60 years testing the effect of Aphrodit capsules containing Tribulus terrestris, ginger, saffron, and Cinnamomum on menopausal symptoms. The intervention group showed a statistically significant reduction in menopausal symptoms compared to the placebo group.

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2016·Journal of evidence-based complementary & alternative medicine·Nasrin Babadaei Samani, Azam Jokar, Mahmood Soveid, et al

Efficacy of the Hydroalcoholic Extract of Tribulus terrestris on the Serum Glucose and Lipid Profile of Women With Diabetes Mellitus: A Double-Blind Randomized Placebo-Controlled Clinical Trial.

RCTn = 98Nutrition

Double-blind randomized placebo-controlled trial evaluating the efficacy of hydroalcoholic extract of Tribulus terrestris on serum glucose and lipid profile in 98 women with type 2 diabetes mellitus. T terrestris significantly lowered blood glucose, total cholesterol, and LDL levels compared to placebo, but had no significant effect on triglyceride and HDL levels.

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2016·Revista brasileira de ginecologia e obstetricia : revista da Federacao Brasileira das Sociedades de Ginecologia e Obstetricia·Sóstenes Postigo, Sônia Maria Rolim Rosa Lima, Silvia Saito Yamada, et al

Assessment of the Effects of Tribulus Terrestris on Sexual Function of Menopausal Women.

RCTn = 60Sexual Health

This randomized, double-blind, placebo-controlled clinical trial assessed the effects of Tribulus terrestris on sexual function in 60 postmenopausal women with sexual dysfunction. The Tribulus group showed significant improvements in desire, sexual interest, foreplay, arousal, and comfort in sexual intercourse compared to the placebo group. There was no significant difference in orgasm and sexual satisfaction. Tribulus terrestris was found to be effective in treating sexual problems among menopausal women after 90 days of treatment.

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2016·Archivio italiano di urologia, andrologia : organo ufficiale [di] Societa italiana di ecografia urologica e nefrologica·Salvatore Sansalone, Giorgio Ivan Russo, Nicola Mondaini, et al

A combination of tryptophan, Satureja montana, Tribulus terrestris, Phyllanthus emblica extracts is able to improve sexual quality of life in patient with premature ejaculation.

RCTSexual Health

RCT evaluating the efficacy of a combination of tryptophan, Satureja montana, Tribulus terrestris, and Phyllanthus emblica extracts in improving sexual quality of life in patients with premature ejaculation. Significant improvements in IELT and PEDT scores were observed in the treatment group compared to controls.

PubMedRead on PubMed
2014·BioMed research international·Salvatore Sansalone, Rosario Leonardi, Gabriele Antonini, et al

Alga Ecklonia bicyclis, Tribulus terrestris, and glucosamine oligosaccharide improve erectile function, sexual quality of life, and ejaculation function in patients with moderate mild-moderate erectile dysfunction: a prospective, randomized, placebo-controlled, single-blinded study.

RCTn = 177Sexual Health

This study evaluated the efficacy of oral therapy with alga Ecklonia bicyclis, Tribulus terrestris, and glucosamine oligosaccharide (Tradamix TX1000) in 177 patients with mild-moderate erectile dysfunction. Significant improvements were observed in erectile and ejaculation function, sexual quality of life, and various IIEF measures in the treatment group compared to placebo.

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2014·Daru : journal of Faculty of Pharmacy, Tehran University of Medical Sciences·Elham Akhtari, Firoozeh Raisi, Mansoor Keshavarz, et al

Tribulus terrestris for treatment of sexual dysfunction in women: randomized double-blind placebo - controlled study.

RCTn = 60Sexual Health

Randomized double-blind placebo-controlled trial assessing the safety and efficacy of Tribulus terrestris in 67 women with hypoactive sexual desire disorder. The Tribulus terrestris group showed significant improvement in FSFI scores, including desire, arousal, lubrication, satisfaction, and pain, compared to placebo.

PubMedRead on PubMed
2012·BMC surgery·Fabrizio Iacono, Domenico Prezioso, Ester Illiano, et al

Sexual asthenia: Tradamixina versus Tadalafil 5 mg daily.

RCTn = 70Sexual Health

RCT comparing the effects of Tradamixina, a natural compound, to tadalafil 5 mg daily on male sexual function in elderly men. Tradamixina improved libido and erectile function without the side effects associated with tadalafil.

PubMedRead on PubMed
2012·Ayu·Thirunavukkarasu M. Sellandi, A. Thakar, M. Baghel

Clinical study of Tribulus terrestris Linn. in Oligozoospermia: A double blind study

RCTSexual Health Mens Health

Double-blind, randomized, placebo-controlled study on the effect of Gokshura (Tribulus terrestris) in managing Oligozoospermia. Gokshura granules showed 78.11% improvement in sexual health and semen analysis compared to placebo.

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2025·OMICS: A Journal of Integrative Biology·Riya Vishwakarma, Abel Koshy, Haritha Kalath, et al

Toward Natural Products-Based Drug Development for Erectile Dysfunction: A Molecular Candidate from Plant Tribulus terrestris Identified by an Integrated In Silico Study

StudySexual Health

In silico study screening phytochemicals from Tribulus terrestris to identify molecular candidates that inhibit PDE5 for erectile dysfunction treatment. Diosgenin,dehydro, ruscogenin, and hecogenin showed higher negative binding affinities compared to sildenafil, suggesting potential for drug discovery with possibly fewer side effects.

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2025·Bulletin Samara State Agricultural Academy·V. N. Setin, Alexander N. Zagoryanskiy, Irina A. Volodina, et al

The experience of the introduction of creeping anchors (Tribulus terrestris L.) in the Middle Volga region

Study

The paper presents the results of introducing and studying the medicinal plant Tribulus terrestris L. in the Middle Volga region. It discusses the growing season, flowering, and productivity indicators of the plant in different years and populations.

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2025·BMC Plant Biology·Yasamin Dabaghkar, Ghasem Eghlima, Hossein Behboudi, et al

Agro-morphological characterization and assessment of metabolic profiling and anticancer activities in various tribulus (Tribulus terrestris L.) populations

StudyInflammation Brain Health Physique Longevity Immunity

The study assessed the agro-morphological and phytochemical diversity of Tribulus terrestris populations from 24 regions in Iran, focusing on their anti-proliferative activity against human prostate cancer cells (PC3). The extracts showed significant cytotoxicity with IC50 values ranging from 15.02 to 27.11 µg/ml, indicating potential anticancer properties.

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2025·FLORA AND FAUNA·Varsha Sharma, Suman Suman, Poonam Meena

Phytochemical Screening of Some Primary and Secondary Metabolites of Medicinal Plant Tribulus terrestris in Jaipur District of Rajasthan, India

Study

Pharmacognostical study of Tribulus terrestris evaluating primary and secondary metabolites in different plant parts. Leaves contained the highest amount of alkaloids, flavonoids, and total soluble sugar, while fruits had more proteins and total phenolic content, and roots were rich in tannins and total lipids.

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2024·Nutrients·Patrick Diel, Peter WR Lemon, Juan F. García, et al

Tribulus terrestris and Sport Performance: A Quantitative and Qualitative Evaluation of Its Advertisement and Availability via Online Shopping in Six Different Countries

Cross-sectional

Cross-sectional study evaluating the availability and advertisement of Tribulus terrestris via online shopping in six countries. The study found that the supplement is mainly marketed in Russia, Ukraine, and Spain, with claims of sports performance benefits and potential improvements in male hormone levels and sexual function.

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2024·Natural Product Research·Mohan Lal, Dipankar Sutradhar

A comprehensive analysis of phytochemicals, antioxidant, anti-inflammatory, antibacterial, antifungal and phytoestrogenic properties of different parts of Tribulus terrestris

Study

The study analyzed different parts of Tribulus terrestris for phytochemicals and biological activities. Ethanol-water was best for extracting saponins and polyphenols, ethanol for flavonoids, and methanol for alkaloids. Leaf extracts showed strong antibacterial and phytoestrogenic activity, while root, stem, and leaf extracts exhibited antifungal activity.

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2024·Phytochemical analysis : PCA·Shuai Wang, De-Feng Du, Fei Li, et al

"UHPLC-Q-TOF/MS-chemometrics-network pharmacology" integrated strategy to discover quality markers of raw and stir-fried Fructus Tribuli and process optimization of stir-fried Fructus Tribuli.

Study

The study aimed to identify quality markers of raw and stir-fried Fructus Tribuli using UHPLC-Q-TOF/MS and optimize the processing technology of stir-fried Fructus Tribuli. Terrestrosin D and Terrestrosin K were identified as quality markers, and optimal processing conditions were established.

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2024·Ultrasonics Sonochemistry·Stefan Kuvendziev, Isidora Dimitrievska, Martin Stojchevski, et al

ANN modeling and RSM optimization of ultrasound-assisted extraction of protodioscin-rich extracts from Tribulus terrestris L.

Study

The study focuses on optimizing the ultrasound-assisted extraction process of protodioscin-rich extracts from Tribulus terrestris L. using ANN modeling and RSM optimization. The process aimed to maximize extraction yield and protodioscin content, achieving a maximal yield of 31% and protodioscin content of 5.9 mg/g dry plant matrix.

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2023·International Journal of Applied Pharmaceutics·Lokesh Barde, P. Suruse, S. Agrawal, et al

DESIGN, DEVELOPMENT AND FABRICATION OF MOUTH-DISSOLVING TABLETS CONTAINING EXTRACT OF TRIBULUS TERRESTRIS FOR THE TREATMENT OF HYPERTENSION

Study

The study focuses on the design, development, and fabrication of mouth-dissolving tablets containing extract of Tribulus terrestris for hypertension treatment. The tablets were evaluated for various characteristics, including hardness, friability, disintegration time, and in vitro drug release, showing potential for improved bioavailability and patient compliance.

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2023·Journal of Biomolecular Structure and Dynamics·C. Vinay, C. Mehta, Chandrakanth Bhat, et al

Integrated LC-MS/MS and network pharmacology approach for predictingactive ingredients and pharmacological mechanisms of Tribulus terrestris L. against cardiac diseases

StudyHeart Health

The study used an integrated approach of metabolomics and network pharmacology to identify active ingredients and potential mechanisms of action of Tribulus terrestris L. for treating cardiac diseases. A total of 55 putative compounds were identified, with several showing direct interaction with cardiac disease-related protein targets.

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2023·chemistry of plant raw material·Temurbek Atahanovich Hajibaev, T. F. Ibragimov, Равшанжон Муратджанович Халилов

OPTIMAL CONDITIONS FOR CLEANING AND DRYING FUROSTANOL SAPONINS FROM TRIBULUS TERRESTRIS

Study

The paper describes the optimal conditions for cleaning and drying furostanol saponins from Tribulus terrestris using liquid-liquid extraction and spray drying techniques. The developed technology ensures a high yield of 'Dry extract Tribulus' with at least 45% furostanol saponins.

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2020·Pharmacy & Pharmacology·A. Affaf, Y. Karpenko, D. K. Gulyaev, et al

Phytochemical study of Tribulus Terrestris L.

Study

Phytochemical study of Tribulus terrestris L. from different geographical zones, focusing on saponins and elemental composition. Dioscin and protodioscin were identified, with varying concentrations depending on the habitat. Potassium and calcium were the predominant macroelements.

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2017·Gynecological endocrinology : the official journal of the International Society of Gynecological Endocrinology·Fabiene Bernardes Castro Vale, Karla Zanolla Dias de Souza, Camilla Russi Rezende, et al

Efficacy of Tribulus Terrestris for the treatment of premenopausal women with hypoactive sexual desire disorder: a randomized double-blinded, placebo-controlled trial.

RCTn = 40Sexual Health

RCT evaluating the efficacy of Tribulus terrestris in treating premenopausal women with hypoactive sexual desire disorder. The study found significant improvements in sexual function scores and increased levels of free and bioavailable testosterone in the treatment group compared to placebo.

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2016·Menopause (New York, N.Y.)·Karla Zanolla Dias de Souza, Fabiene Bernardes Castro Vale, Selmo Geber

Efficacy of Tribulus terrestris for the treatment of hypoactive sexual desire disorder in postmenopausal women: a randomized, double-blinded, placebo-controlled trial.

RCTn = 36Sexual Health Hormone Health

RCT evaluating the efficacy of Tribulus terrestris for treating hypoactive sexual desire disorder in postmenopausal women. The study found significant improvements in sexual desire, arousal, lubrication, pain, and anorgasmia in the T terrestris group, along with increased free and bioavailable testosterone levels.

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2016·Journal of dietary supplements·Mohamed Farid Roaiah, Yasser Ibrahim Elkhayat, Mohamed Ahmed Abd El Salam, et al

Prospective Analysis on the Effect of Botanical Medicine (Tribulus terrestris) on Serum Testosterone Level and Semen Parameters in Males with Unexplained Infertility.

RCTn = 30Sexual Health -

RCT evaluating the effect of Tribulus terrestris on serum testosterone and semen parameters in 30 males with unexplained infertility. No statistically significant differences were observed in testosterone levels, LH, or semen parameters before and after treatment, indicating Tribulus terrestris was ineffective for idiopathic infertility.

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2014·Actas urologicas espanolas·C A Santos, L O Reis, R Destro-Saade, et al

Tribulus terrestris versus placebo in the treatment of erectile dysfunction: A prospective, randomized, double blind study.

RCTn = 30Sexual Health -

Prospective, randomized, double-blind, placebo-controlled study evaluating the effects of Tribulus terrestris on erectile dysfunction and serum testosterone levels in 30 healthy men. The study found no significant difference between Tribulus terrestris and placebo in improving erectile dysfunction symptoms or serum testosterone levels.

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2011·Clinical therapeutics·Gairik Sengupta, Avijit Hazra, Anup Kundu, et al

Comparison of Murraya koenigii- and Tribulus terrestris-based oral formulation versus tamsulosin in the treatment of benign prostatic hyperplasia in men aged >50 years: a double-blind, double-dummy, randomized controlled trial.

RCTn = 46Mens Health

A double-blind, double-dummy, randomized controlled trial compared the effectiveness of an oral formulation containing Murraya koenigii and Tribulus terrestris with tamsulosin in treating symptomatic benign prostatic hyperplasia (BPH) in men over 50. Both treatments significantly reduced the International Prostate Symptom Score (IPSS), but no significant difference was found between the groups. The plant formulation also significantly reduced prostate volume.

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2010·Reproduction in domestic animals = Zuchthygiene·S Frydrychová, L Opletal, K Macáková, et al

Effects of herbal preparation on libido and semen quality in boars.

RCTn = 10Sexual Health

RCT investigating the effects of a herbal preparation (PHE) containing Eurycoma longifolia, Tribulus terrestris, and Leuzea carthamoides on libido and semen quality in boars. The experimental group showed increased libido and improvements in some semen quality parameters compared to the control group.

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2007·Journal of strength and conditioning research·Shane Rogerson, Christopher J Riches, Carl Jennings, et al

The effect of five weeks of Tribulus terrestris supplementation on muscle strength and body composition during preseason training in elite rugby league players.

RCTn = 22Physical Performance -Physique -

RCT of 22 elite male rugby league players assessing the effect of 5 weeks of Tribulus terrestris supplementation on muscle strength, body composition, and urinary T/E ratio. No significant differences were found between the Tribulus terrestris and placebo groups in strength, fat free mass, or urinary T/E ratio.

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2005·Journal of ethnopharmacology·V K Neychev, V I Mitev

The aphrodisiac herb Tribulus terrestris does not influence the androgen production in young men.

RCTn = 21Mens Health -

RCT investigating the influence of Tribulus terrestris extract on androgen metabolism in 21 healthy young men. No significant differences were found in serum testosterone, androstenedione, or luteinizing hormone levels between the Tribulus terrestris supplemented groups and controls.

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2000·International journal of sport nutrition and exercise metabolism·J Antonio, J Uelmen, R Rodriguez, et al

The effects of Tribulus terrestris on body composition and exercise performance in resistance-trained males.

RCTn = 15Physical Performance -Physique -

RCT investigating the effects of Tribulus terrestris on body composition and exercise performance in resistance-trained males. No changes were observed in body weight, percentage fat, total body water, dietary intake, or mood states. Muscle endurance increased in the placebo group for bench and leg press exercises, while the tribulus group only showed an increase in leg press strength.

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2025·Current Issues in Molecular Biology·K. Alzahrani

Tribulus terrestris Fruit Extract: Bioactive Compounds, ADMET Analysis, and Molecular Docking with Penicillin-Binding Protein 2a Transpeptidase of Methicillin-Resistant Staphylococcus epidermidis

In vitro

The study evaluated Tribulus terrestris methanolic HPLC fractions for bioactive compounds and PBP2a transpeptidase inhibitors against methicillin-resistant Staphylococcus epidermidis (MRSE). The F02 fraction demonstrated potential activity against MRSE isolates, and two compounds were identified as potential PBP2a transpeptidase inhibitors through molecular docking.

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2025·Basic & Clinical Pharmacology & Toxicology·Marwan A Ibrahim, Barga Aboukhezam, Fayzah A Aboubakr, et al

In Vivo and In Silico Dissection of Triclosan‐Induced Reproductive Toxicity: Protective Potentials of Nanoselenium and Phytotherapy

Animal study

The study investigated the protective efficacy of clomiphene citrate, conventional selenium, nanoselenium, and Tribulus terrestris extract against triclosan-induced toxicity in male rats. Treatments with selenium (conventional and nanoforms) and Tribulus terrestris extract significantly mitigated reproductive and hepatic function impairments, oxidative stress, and tissue damage caused by triclosan exposure.

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2024·Dental Research Journal·Ali Azarm, Fatemeh Ayoobi, M. Zare-Bidaki, et al

Antibacterial and antibiofilm activities of tribulus terrestris methanolic extract against Streptococcus mutans, Streptococcus sobrinus, and Lactobacillus acidophilus: An in vitro study

In vitro

In vitro study evaluating the antibacterial and antibiofilm activities of Tribulus terrestris extract against Streptococcus mutans, Streptococcus sobrinus, and Lactobacillus acidophilus. The extract showed good time-dependent antibacterial and antibiofilm activity, as well as acid production inhibition, against these cariogenic bacteria.

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2024·BMC Veterinary Research·Aya F. Matter, W. Raslan, Eman I. Soror, et al

Comparable to 17α- methyl testosterone, dietary supplements of Tribulus terrestris and Mucuna pruriens promote the development of mono-sex, all-male tilapia fry, growth, survival rate and sex-related genes (Amh, Sox9, Foxl2, Dmrt1)

Animal study

The study evaluated the effects of Tribulus terrestris and Mucuna pruriens on inducing all-male tilapia. The plant extracts significantly increased growth metrics and survival rates, improved the proportion of males, and altered sex-related gene expression compared to controls. The study concluded that these extracts can induce sex reversal in tilapia without negative health impacts.

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2024·Journal of Biomedicine and Biosensors·S. Rahim, A. Al-Nahi

Protective Effects of Tribulus Terrestris L Extract Against Kidney Injury Induced By Sorafinib in Rats

Animal study

Animal study evaluating the protective effects of Tribulus terrestris fruit aqueous extract against nephrotoxic effects of Sorafinib in rats. The extract reduced elevated levels of urea, uric acid, and creatinine caused by Sorafinib, indicating a protective effect against kidney injury.

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2024·Journal of Medical and Life Science·Ghufran, Isewid, M. Madhloom

Study the Potential Role of Tribulus terrestris Extract on Cancer Treatment

Animal studyInflammation Longevity

The study assessed the chemotherapeutic anti-tumor ability of Tribulus terrestris extract against mammary carcinogenesis in Swiss albino mice with Ehrlich ascites carcinoma. Results showed reduced oxidative stress, increased antioxidant levels, restored liver and kidney functions, and inhibited tumor growth and neovascularization.

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2024·Current Bioactive Compounds·R. Ghai, Karishma Chauhan, K. Nagarajan, et al

Therapeutic Synergistic Potential of Tribulus terrestris and Asparagus racemosus in Treating Hypogonadism in Orchiectomized Rats

Animal studyMens Health

Animal study evaluating the combined effects of Tribulus terrestris and Asparagus racemosus on hypogonadism in orchiectomized rats. The combination improved testosterone levels compared to Tribulus terrestris alone and the standard drug Tentex forte.

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2024·Clinical and Experimental Pharmacology and Physiology·Xing-Wang Cheng, Yuantao Su, Ningzheng Dong, et al

Gross saponins of Tribulus terrestris attenuate rheumatoid arthritis by promoting apoptosis of fibroblast‐like synoviocytes and reducing inflammation by inhibiting MAPK signalling pathway

In vitroInflammation Joint Bone Health

The study investigates the effects of gross saponins of Tribulus terrestris (GSTT) on rheumatoid arthritis fibroblast-like synoviocytes (RA-FLSs) and inflammation. GSTT promotes apoptosis of RA-FLSs, reduces pro-inflammatory cytokine levels, and inhibits MAPK signalling pathway, showing potential as a therapeutic intervention for rheumatoid arthritis.

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2024·Cureus·Mohammed Yaseen Malik, Arockia Alex, Azhagu Madhavan Sivalingam, et al

Evaluation of the Phytochemical Screening of Methanolic Seed Extracts of Tribulus terrestris: An In Vitro Application of Anti-cancer, Anti-oxidant, and Anti-microbial Activities

In vitroInflammation Immunity Longevity

In vitro study of methanolic seed extracts of Tribulus terrestris evaluating its anticancer, antioxidant, anti-inflammatory, and antimicrobial activities. The extract showed concentration-dependent cytotoxicity against oral cancer cells, pro-apoptotic effects, and significant antioxidant and antimicrobial activities.

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2024·Research Journal of Pharmacy and Technology·T. Khajibaev, Feruza Egamova, D. Mutalova, et al

Hypoazotemic action of dry extracts from the waste of Tribulus terrestris

Animal study

The study investigates the hypoazotemic effect of dry extracts from Tribulus terrestris meal on kidney function in animal models of acute kidney failure. The extract, named Triburenal, significantly reduced urea and creatinine levels in the blood serum of rats, outperforming the reference drug Cynaroside.

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2024·Pakistan Journal of Biochemistry and Biotechnology·M. Afzal, Waseem Akhtar Qureshi, Qijing Liu, et al

Phytochemical Analysis and Antibacterial Activity of Tribulus terrestris from Cholistan Desert

In vitro

The study investigated the phytochemical composition and antibacterial activity of Tribulus terrestris from the Cholistan Desert. Methanolic extracts of various plant parts were tested against bacterial strains, showing varying levels of inhibitory activity, with the highest against Klebsiella pneumoniae.

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2024·BIO Web of Conferences·Shaima Ahmed Rahim, Alaa Shakir Al-Nahi

Role of Tribulus terrestris as an antioxidant against Oxidative Stress Induced by Sorafenib in adult male rats

Animal studyInflammation

The study evaluated the protective effects of Tribulus terrestris fruit aqueous extract against hepatotoxic effects induced by Sorafenib in adult male rats. Tribulus terrestris was found to normalize liver enzyme levels and improve antioxidant markers, suggesting a protective role against oxidative stress and liver damage.

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2023·Biomedical chromatography : BMC·Xingxing Li, Wenjun Guo, Liang Zhao, et al

Investigating the in vivo effect of Tribulus terrestris extract in MCAO rats by using LC-MS-based metabolomics combined with the molecular docking.

Animal studyBrain Health

The study investigates the protective effect of Tribulus terrestris extract (TT15) on middle cerebral artery occlusion (MCAO) rats using metabolomics and molecular docking. TT15 was found to restore MCAO-induced serum metabolite changes and modulate multiple metabolic pathways, confirming its efficacy in reducing infarct volume and neurological defects.

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2023·Molecules·Abdur Rahman, Gauhar Rehman, Nasrullah Shah, et al

Biosynthesis and Characterization of Silver Nanoparticles Using Tribulus terrestris Seeds: Revealed Promising Antidiabetic Potentials

In vitroNutrition

The study focused on the biosynthesis of silver nanoparticles using Tribulus terrestris seed extract and their characterization. The synthesized nanoparticles demonstrated promising antidiabetic properties in vitro through glucose uptake, glucose adsorption, and alpha-amylase assays. In vivo studies on diabetic mice showed significant improvements in blood sugar levels and biochemical markers after treatment with the nanoparticles.

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2023·Journal of separation science·Xiaohang Xu, Wenjun Guo, Liang Zhao, et al

Exploring the in vitro anti-inflammatory activity of gross saponins of Tribulus terrestris L. fruit by using liquid chromatography-mass spectrometry-based cell metabolomics approach.

In vitroInflammation

In vitro study investigating the anti-inflammatory activity of gross saponins from Tribulus terrestris L. fruit using a lipopolysaccharide-induced BV2 cell model. The saponins reduced nitric oxide release and secretion of inflammatory cytokines, with effects on metabolic pathways such as glycerophospholipid and tryptophan metabolism.

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2023·Nutrients·M. Povydysh, M. Titova, D. Ivkin, et al

The Hypoglycemic and Hypocholesterolemic Activity of Dioscorea deltoidea, Tribulus terrestris and Panax japonicus Cell Culture Biomass in Rats with High-Fat Diet-Induced Obesity

Animal studyNutrition Physique Heart Health

Animal study testing the effects of Dioscorea deltoidea, Tribulus terrestris, and Panax japonicus cell culture biomass on rats with high-fat diet-induced obesity. The biomass reduced weight gain, fat mass, glucose, and cholesterol levels, with Dioscorea deltoidea showing the most significant effects, sometimes exceeding those of liraglutide.

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2023·ACS Omega·Hongming Zhang, Wenjun Guo, Xingxing Li, et al

Supplementation with Tribulus Terrestris Extract Exhibits Protective Effects on MCAO Rats via Modulating Inflammation-Related Metabolic and Signaling Pathways

Animal studyInflammation

The study investigated the protective effects of Tribulus terrestris extract on ischemic stroke in MCAO rat models. GSTTF administration decreased brain infarction volume and improved neurobehavioral scores, with metabolomics revealing involvement of inflammation-related metabolic pathways. GSTTF reduced TNF-α and IL-6 levels and inhibited the TLR4/MyD88/NF-κB signaling pathway, suggesting anti-inflammatory mechanisms.

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2023·Caryologia·Ali Bouzekri, M. Nassar, S. Slimani, et al

Cytogenetic effects of Tribulus terrestris L. on meristematic cells of Allium cepa L. and Vicia faba L.

In vitro

The study examines the cytotoxic and genotoxic effects of Tribulus terrestris on meristematic cells of Allium cepa and Vicia faba. Extracts were tested at various concentrations, showing significant decreases in root length and mitotic index, and increases in chromosomal aberrations, indicating genotoxicity.

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2022·Journal of Hunan University Natural Sciences·Sidra Yasmeen, S. Yasmeen, Fatima Qamar, et al

Acute and Sub-Acute Toxicity of Spray-Dried Extract of Tribulus Terrestris

Animal study

The study investigated the acute and sub-acute toxicity of spray-dried extract of Tribulus terrestris in Wistar rats. No signs of morbidity or mortality were observed in acute toxicity tests, and mild, non-significant variations were noted in sub-acute toxicity tests at higher doses. The extract was deemed safe for use in pharmaceutical formulations.

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2020·ACS Omega·U. Saleem, Zunera Chauhdary, Zohaib Raza, et al

Anti-Parkinson’s Activity of Tribulus terrestris via Modulation of AChE, α-Synuclein, TNF-α, and IL-1β

Animal studyBrain Health

The study investigated the anti-Parkinson’s activity of Tribulus terrestris methanol extract (TTME) in a rat model. TTME showed significant improvement in reversing catatonia, muscular strength, locomotor functions, and exploration in a dose-dependent manner. It also restored antioxidant enzyme levels and decreased acetylcholinesterase levels, indicating neuroprotection.

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2019·Health·Trama Francesco, R. Filippo, Romeo Giuseppe, et al

“Tradamixina TP” Compound in Monotherapy for Three Months in Patients with LUTS: Observational Study on Improvement of Urinary Symptoms and Sexual Function in Men

Observationaln = 100Sexual Health Mens Health

Observational study of 100 men with LUTS evaluating the effect of Tradamixina TP, a compound containing Ecklonia Bicyclis, Tribulus terrestris, Chitosan, and Serenoa repens. After 3 months, improvements were observed in sexual function, LUTS, and a decrease in serum PSA levels.

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2026·Frontiers in Pharmacology·Xiaomu Wang, Xiaomin Liu, Yan-Ling Zeng, et al

Tribulus terrestris L.: a medicinal plant with promising therapeutic potential for skin diseases

ReviewSkin Hair Health

This review summarizes current research on the protective effects of Tribulus terrestris L. in skin diseases. It highlights the plant's anti-inflammatory, antioxidant, antibacterial properties, and potential efficacy against conditions like atopic dermatitis, acne, and vitiligo. The review calls for more rigorous clinical trials to verify its efficacy and safety.

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2025·Tikrit Journal of Pharmaceutical Sciences·Shakir Jasim Mohammed AL-Hathoot, Marwah Isam Sulaiman Musa

Anti-Hepatotoxic Effects of Aqueous and alcoholic Extracts of Tribulus terrestris Fruits and FertiPlus® on Albino Rats Exposed to Sulfasalazine

Animal studyn = 35

Animal study evaluating the anti-hepatotoxic effects of aqueous and alcoholic extracts of Tribulus terrestris fruits and FertiPlus® on male albino rats exposed to sulfasalazine. Treatment with these extracts resulted in significant improvements in liver enzyme levels and histological structure, with the aqueous extract and FertiPlus® showing the strongest hepatoprotective effects.

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2025·Babcock University Medical Journal·Rawan Al-Hamadani, H. Diab, Mohammed Al-hamdani

Protective Effects of Tribulus terrestris Extract on Methotrexate-Induced Liver Damage in Male Rabbits: An Experimental study

Animal studyn = 20

This experimental study investigated the protective effects of Tribulus terrestris extract against methotrexate-induced liver damage in male rabbits. The extract helped restore blood parameters and liver enzyme levels affected by methotrexate, indicating its protective role against hepatotoxicity.

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2025·Notulae Scientia Biologicae·Haiyan Zhang, Yue Kuang, M. H. Shahrajabian, et al

Study of puncture vine (Tribulus terrestris L.), and the important roles of its chemical ingredients

ReviewSexual Health Immunity Heart Health Inflammation Brain Health

This narrative review presents the phytochemical and pharmacological data of Tribulus terrestris, highlighting its aphrodisiac, anthelmintic, hypolipidemic, immunomodulatory, diuretic, and hepatoprotective potentials. It also discusses its antibacterial, anti-inflammatory, anticancer, antidiabetic, antiurolithic, and cardiotonic properties, emphasizing its medicinal importance.

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2025·International Journal of Indigenous Herbs and Drugs·Radhika Sharma, Shilpy Gupta, K. Vijendra, et al

Hrisvapanchamool: A Potential Ayurvedic Formulation for Management of Balshosha (Primary acute malnutrition)

ReviewNutrition Immunity

Narrative review of Hrisvapanchamool, an Ayurvedic formulation, for managing primary acute malnutrition. The formulation, consisting of roots from five medicinal plants, is documented for its benefits in promoting healthy metabolism and immunity in malnourished children. The review highlights the therapeutic potential of its phytochemical constituents and supports its role in enhancing nutritional status.

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2025·Pharmacognosy Journal·Baigalmaa Dovchinsuren, Ariunaa Damdinsuren, Bolor Bayarkhuu, et al

Tribulus terrestris L. in traditional Mongolian medicine: Medicinal Applications, Phytochemistry, Pharmacology

ReviewPhysical Performance Sexual Health Longevity

Narrative review of Tribulus terrestris L. in traditional Mongolian medicine, highlighting its historical use for kidney protection, urination promotion, and energy boosting. Modern research supports its kidney-protective, diuretic, and sexual function-enhancing effects, with potential anti-aging benefits.

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2025·International Ayurvedic Medical Journal·Shubham Khandekar, B. Ram, Premlata, et al

GOKSHURA (TRIBULUS TERRESTRIS LINN.): A COMPREHENSIVE REVIEW OF ITS AYURVEDIC SIGNIFICANCE, PHYTOCONSTITUENT ANALYSIS, AND MODERN PHARMACOLOGICAL POTENTIAL

ReviewInflammation Physical Performance Gut Health

This narrative review examines Gokshura (Tribulus terrestris Linn.) from both Ayurvedic and modern scientific perspectives, highlighting its phytochemical composition and pharmacological actions. The review discusses its diuretic, anti-inflammatory, nephroprotective, and antioxidant effects, with evidence supporting its use in managing urinary disorders and inflammation.

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2024·Pharmaceuticals·S. Bouabdallah, Mona H. Ibrahim, Ion Brînza, et al

Anxiolytic and Antidepressant Effects of Tribulus terrestris Ethanolic Extract in Scopolamine-Induced Amnesia in Zebrafish: Supported by Molecular Docking Investigation Targeting Monoamine Oxidase A

Animal studyn = 48Stress Mood Brain Health

The study evaluated the effects of Tribulus terrestris ethanolic extract on scopolamine-induced amnesia in zebrafish. Tribulus terrestris exhibited anxiolytic and antidepressant effects, attenuating cognitive deficits in zebrafish. Molecular docking suggested flavonoids in the extract could interact with monoamine oxidase A, potentially improving anxiety-related disorders.

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2024·BIO Web of Conferences·Shaima Ahmed Rahim, Alaa Shakir Al-Nahi

Cytogenetic effect of Tribulus terrestris fruit aqueous extract on Chromosome Aberrations and Mitotic Index in Sorafinib treated albino male rats

Animal studyn = 32

The study explored the protective impact of Tribulus terrestris aqueous extract against Sorafinib cytotoxicity in adult male albino rats. Tribulus terrestris showed potential cytoprotective effects, decreasing chromosomal aberrations and increasing the mitotic index compared to the Sorafinib group.

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2024·Heliyon·Muhammad Saeed, Mahzaib Munawar, Jannat Bi Bi, et al

Promising phytopharmacology, nutritional potential, health benefits, and traditional usage of Tribulus terrestris L. herb

ReviewImmunity Sexual Health Heart Health Inflammation Brain Health Mens Health

Narrative review of Tribulus terrestris (Gokharu), highlighting its traditional use and therapeutic effects such as immunomodulatory, aphrodisiac, cardioprotective, antidiabetic, anti-inflammatory, and testosterone-boosting properties. The review emphasizes its potential in treating human and animal ailments, including fertility-related disorders.

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2024·Biomedical Physics & Engineering Express·Maganti Raghav Prasaad Choudary, Muthuvel Surya, Muthupandian Saravanan

Green synthesis of cerium oxide nanoparticles using Tribulus terrestris: characterization and evaluation of antioxidant, anti-inflammatory and antibacterial efficacy against wound isolates

In vitroInflammation Immunity

The study synthesized cerium oxide nanoparticles using Tribulus terrestris extract and evaluated their antioxidant, anti-inflammatory, and antibacterial properties. The nanoparticles showed significant antioxidant and anti-inflammatory activity and were effective against multi-drug resistant bacterial strains.

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2024·International Journal of All Research Education and Scientific Methods·Rutuja R . Waghmode, Harshali Thakare, Abhishek Kumar Sen, et al

Re- Evaluating the Pharmacological Activities and Therapeutic Uses of Tribulus Terrestris Linn (Gokharu)

ReviewSexual Health Inflammation

The paper reviews the pharmacological activities and therapeutic uses of Tribulus Terrestris, known as gokharu, focusing on its anti-inflammatory, aphrodisiac, and diuretic effects. It evaluates the extract's potential in relieving sexual dysfunction and improving quality of life.

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2024·The American Journal of Case Reports·Ross Huff, K. Karpinska-Leydier, G. Maddineni, et al

Rhabdomyolysis Risk: The Dangers of Tribulus terrestris, an Over-the-Counter Supplement

Case reportSexual Health Energy

Case report of a 71-year-old man who developed rhabdomyolysis after taking the over-the-counter supplement Tribulus terrestris while on long-term atorvastatin. The case highlights the potential for serious adverse effects due to interactions between Tribulus terrestris and medications metabolized by CYP 3A4.

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2024·Фармацевтичний часопис·S. Bohatu, B. Prystupa, V. Kresyun, et al

CURRENT CONCEPTS ABOUT THE PHYTOCHEMICAL COMPOSITION AND PHARMACOLOGICAL ACTIVITY OF GOATHEAD (TRIBULUS TERRESTRIS L.)

ReviewSexual Health Heart Health Inflammation Immunity Mood

The paper critically analyzes and summarizes scientific literature on the phytochemical composition and pharmacological activity of Tribulus terrestris. It highlights the plant's use in traditional medicine for its diuretic, sedative, antibacterial, anti-inflammatory, and sexual health benefits, among others.

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2023·Asian Journal of Andrology·M. D. Da Silva, Gabriela F. Buys-Gonçalves, João Henrique Duque Estrada, et al

What is the effect of Tribulus terrestris on testicular morphology and sperm production? An experimental study in the rat model

Animal studyn = 20Sexual Health -

Experimental study in rats to assess the impact of Tribulus terrestris on testicular morphology and sperm production. No significant differences were found in spermatozoa concentration, motility, and viability between the control and treated groups. Some subtle changes in seminiferous epithelium height and tubular lumen surface density were observed.

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2023·Veterinary Research Forum·Mohammad Moein Sabzeie, E. Ayen, A. Soleimanzadeh, et al

Tribulus terrestris aqueous extract supplementation effects on sperm characteristics and anti-oxidant status during chilled storage of canine semen

Animal studyn = 3

The study investigated the effects of Tribulus terrestris aqueous extract (TTAE) on sperm characteristics and antioxidant status during chilled storage of canine semen. It found that supplementing the semen extender with 40.00 µg mL-1 TTAE improved semen parameters, including motility and viability, and increased antioxidant capacity after 72 hours of storage at 4.00 ˚C.

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2023·International Research Journal of Ayurveda & Yoga·P. Gautam, O. Bairwa, Rajkumar Yadav, et al

Comprehensive Study of Gokshura (Tribulus terrestris Linn.) in Ayurvedic Literature with Special Reference to Mutrakricchra

Review

This narrative review arranges Ayurvedic literature on Gokshura (Tribulus terrestris Linn.) with reference to its use in treating mutrakricchra roga (urinary tract infection). The study highlights its application in various pharmacological preparations for this condition.

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2022·Diabetology·Rasha A. Al-Eisa, H. Tag, Mohamed S. Elnaggar, et al

Evaluation of Tribulus terrestris Extracts Relative to Metformin on Oxidative Stress and Histopathology of the Liver for Diabetic Male Rats

Animal studyn = 36Inflammation

Animal study comparing the effects of Tribulus terrestris extract and metformin on oxidative stress and liver histopathology in diabetic male rats. Tribulus terrestris extract showed more effective antioxidant activity and improvement in liver histology compared to metformin.

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2022·Journal of Herbmed Pharmacology·Suliman Sohaim, Salman A. A. Mohammed, Elham Amin, et al

Date palm seed extract and herbal mixture mitigate gentamicin-induced renal injury in mice: Role of Protease-activated receptors (PARs) and Retinoid X receptor alpha (RXR-α)

Animal studyn = 32

The study examined the nephroprotective effects of date palm seed extract (DPSE) and a herbal mixture against gentamicin-induced renal toxicity in mice. Both treatments significantly improved markers of renal function and modulated RXR-α and PAR-2 protein expression, suggesting protective effects against renal injury.

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2022·International Journal of Clinical Biochemistry and Research·S. Ravichandran, R. Sri, S. Dhandayuthapani, et al

Radical scavenging activity of fruit extracts Tribulus Terrestris with natural antioxidant Ascorbic acid: A comparative study

ReviewWomens Health Sexual Health

Systematic review analyzing the ability of Tribulus terrestris to increase hormonal levels for relief of menopausal symptoms. The review found significant improvements in symptoms such as vaginal lubrication and sensation during intercourse, with an increase in bioavailable testosterone.

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2019·Metabolites·Yang Wang, Wenjun Guo, Yue Liu, et al

Investigating the Protective Effect of Gross Saponins of Tribulus terrestris Fruit against Ischemic Stroke in Rat Using Metabolomics and Network Pharmacology

Animal studyBrain Health

The study investigates the protective effects of gross saponins of Tribulus terrestris fruit (GSTTF) on ischemic stroke in rats using metabolomics and network pharmacology. GSTTF was found to reverse MCAO-induced urine metabolic deviations and highlighted potential therapeutic targets, NOS2 and GSK3B, for ischemic stroke treatment.

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2017·Evidence-based Complementary and Alternative Medicine : eCAM·S. Kang, H. Jung, J. Nam, et al

Effects of the Fruit Extract of Tribulus terrestris on Skin Inflammation in Mice with Oxazolone-Induced Atopic Dermatitis through Regulation of Calcium Channels, Orai-1 and TRPV3, and Mast Cell Activation

Animal studySkin Hair Health Inflammation

The study investigated the effects of Tribulus terrestris fruit extract on oxazolone-induced atopic dermatitis in mice. The extract improved AD symptoms, reduced TEWL and symptom scores, and inhibited skin inflammation by modulating calcium channels and mast cell activation.

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2016·Central European journal of sport sciences and medicine·M. Wilk, M. Michalczyk, Jakub Chycki, et al

Endocrine Responses to Physical Training and Tribulus Terrestris Supplememtation in Middle-Age Men

Studyn = 14Hormone Health

The study evaluated the effects of Tribulus Terrestris supplementation on blood concentrations of testosterone, growth hormone, and IGF-1 in 14 middle-aged men over 12 weeks. Significant effects were observed on testosterone, IGF-1, and growth hormone levels, with increases in fat-free mass and decreases in total body fat and body mass.

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2016·R. Selvaraju, S. Sulochana

In-vitro Growth and Inhibition Studies of Tribulus Terrestris on Calcium Oxalate Monohydrate Crystals

In vitro

In vitro study investigating the effect of Tribulus terrestris extract on the growth and inhibition of calcium oxalate monohydrate crystals. The study found that Tribulus terrestris extract dissolved COM crystals after 21 days, suggesting potential for kidney stone treatment.

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2012·Journal of Pharmacology & Pharmacotherapeutics·Surender Singh, V. Nair, Y. Gupta

Evaluation of the aphrodisiac activity of Tribulus terrestris Linn. in sexually sluggish male albino rats

Animal studySexual Health

The study evaluated the aphrodisiac activity of lyophilized aqueous extract of Tribulus terrestris in sexually sluggish male albino rats. Results showed a dose-dependent improvement in sexual behavior and increased serum testosterone levels with chronic administration, validating its traditional use as a sexual enhancer.

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2011·Indian Journal of Pharmacology·E. E. Jarald, P. Kushwah, S. Edwin, et al

Effect of Unex on ethylene glycol-induced urolithiasis in rats

Animal study

The study evaluated the effectiveness of the Unex capsule, containing Boerhaavia diffusa and Tribulus terrestris, on preventing kidney stones in albino rats using an ethylene glycol-induced urolithiasis model. Unex significantly reduced and prevented urinary stone growth and restored elevated biochemical parameters, urine pH, and increased urine volume.

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2019·Journal of Pharmacy and Pharmacology·M. Akdogan, H. T. Ilçe, A. Bilgili, et al

Protective Effects of Tribulus terrestris, Avena sativa, and White Ginseng Powder on Bone Mineral Density in Hypercholesterolemic Rats

Animal studyJoint Bone Health

Animal study investigating the effects of Tribulus terrestris, Avena sativa, white ginseng, and a combination on cholesterol levels and tibial bone mineral density in hypercholesterolemic rats. White ginseng and the combination significantly prevented serum total cholesterol increase, while the combination significantly prevented serum LDL-C increase. The substances were non-significantly effective in improving tibial BMD levels.

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2018·African Journal of Pharmacy and Pharmacology·A. Istiak, P. Hazra, S. Das, et al

Hypoglycemic, hypolipidemic and kidney protective potential of combined formulation of Tribulus terrestris and Andrographis paniculata in alloxan induced mice

Animal studyn = 30Nutrition Heart Health Inflammation

The study evaluated the effects of Tribulus terrestris and Andrographis paniculata on body weight, blood glucose, lipid profile, and renal protection in alloxan-induced type-1 diabetic mice. The combined formulation improved body weight, lowered blood glucose levels, decreased total cholesterol and plasma triglycerides, and partially improved renal and pancreatic damage.

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2017·Chemistry Central Journal·Wenyi Zhu, Yijie Du, Hong Meng, et al

A review of traditional pharmacological uses, phytochemistry, and pharmacological activities of Tribulus terrestris

Review

This review provides a comprehensive overview of the traditional applications, phytochemistry, and pharmacology of Tribulus terrestris, highlighting its use in energizing, vitalizing, and improving sexual function and physical performance in men. It discusses the bioactive phytochemicals such as saponins and flavonoids responsible for its pharmacological activities.

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2015·Indian Journal of Pharmacology·M. Kumari, P. Singh

Tribulus terrestris ameliorates metronidazole-induced spermatogenic inhibition and testicular oxidative stress in the laboratory mouse

Animal studyn = 30Sexual Health

The study evaluated the protective effects of Tribulus terrestris fruit extract on metronidazole-induced alterations in spermatogenesis, sperm count, and testicular oxidative stress in mice. Tribulus terrestris ameliorated the negative effects of metronidazole, with higher doses completely restoring testicular function and antioxidant enzyme activities.

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2014·Pharmacognosy Reviews·Saurabh Chhatre, T. Nesari, Gauresh S. Somani, et al

Phytopharmacological overview of Tribulus terrestris

Review

Narrative review of Tribulus terrestris, highlighting its use in traditional medicine and its various pharmacological activities such as diuretic, aphrodisiac, immunomodulatory, and anti-inflammatory effects. The review aims to create a database for further research on its phytochemical and pharmacological properties.

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2011·Indian Journal of Pharmacology·K. Bharavi, P. Kishore, C. S. Satish Kumar, et al

Protective effect of an aphrodisiac herb Tribulus terrestris Linn on cadmium-induced testicular damage

Animal studyn = 40Sexual Health

The study investigated the protective effect of Tribulus terrestris Linn (TT) against cadmium-induced testicular damage in rats. In vitro and in vivo studies showed that TT reduced cadmium burden and reversed testicular damage through antioxidant and metal chelator activity, potentially stimulating testosterone production.

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1997·Ancient Science of Life·Ukani, Nanavati Dd, Mehta Nk

A REVIEW ON THE AYURVEDIC HERB TRIBULUS TERRESTRIS L.

Review

Narrative review of the ayurvedic herb Tribulus terrestris, also known as Gokhshura, discussing its chemistry and pharmacology as mentioned in Ayurveda.

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