Research

Piper longum (Long Pepper / Pippali)

23 peer-reviewed studies curated from PubMed and Semantic Scholar.

0
Meta-analyses
1
Systematic reviews
2
RCTs
20
Other studies
Systematic reviews (4%)
RCTs (9%)
Observational (4%)

Studies

Sorted by quality and recency

2019·Journal of ethnopharmacology·Vaishali Yadav, Anuja Krishnan, Divya Vohora

A systematic review on Piper longum L.: Bridging traditional knowledge and pharmacological evidence for future translational research.

Systematic review

This systematic review compiles traditional uses, phytochemistry, and pharmacological profiles of Piper longum, identifying research gaps and future opportunities. It highlights various activities such as anti-inflammatory, analgesic, anti-oxidant, and more, but notes insufficient scientific validation for several traditional claims, necessitating further investigation.

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2026·Food chemistry·Shuwen Geng, Conghui Duan, Ying Ye, et al

Unraveling geographical heterogeneity and spatial distribution of Long pepper fruits: An integrative analytical perspective.

Study

The study examines the geographical variations and distribution patterns of bioactive compounds in Long pepper fruits using multidimensional identification, metabolomics, and spatial metabolomics. It identifies 113 volatile constituents and screens six alkaloids as potential quality markers, providing a basis for quality evaluation and functional food development.

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2026·Chemistry & biodiversity·Lakshminarayana Misro, Anuja S Jeevan, Rahul Maurya, et al

Impact of Regional Variation on the Bioactive Composition and Antioxidant Potential of Piper longum Linn.

ObservationalInflammation

The study investigates the impact of geographical variation on the phytochemical and pharmacognostical properties of Piper longum Linn. Samples from three regions in India showed significant differences in bioactive marker concentrations, with the Ranikhet sample having the highest levels of piperine and piperlongumine, as well as superior antioxidant activity.

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2024·Biotechnology and applied biochemistry·Manaswini Dash, Subhashree Singh, Suprava Sahoo, et al

Metabolite profiling of Piper longum L. fruit volatiles by two-dimensional gas chromatography and time-of-flight mass spectrometry: Insights into the chemical complexity.

Study

The study performed a precise characterization of Piper longum fruit volatiles using advanced two-dimensional gas chromatography-time-of-flight spectrometry. A total of 146 constituents were identified, with sesquiterpene hydrocarbons being the most abundant class.

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2009·Nihon shinkei seishin yakurigaku zasshi = Japanese journal of psychopharmacology·Noriko Yamada, Chie Nishihara, Hiroyuki Yoshimura, et al

[Effects of Piper longum L. on chills in Japanese young women: time-dependent changes in skin surface temperature and its recovery rate following the exposure to mild cold stress].

RCTn = 32Womens Health

RCT investigating the effect of Piper longum on chills in young women. Participants were assigned to low- and high-dose P. longum groups. Piper longum significantly facilitated the recovery of skin surface temperature in participants with chills, but had no effect in those without chills.

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1997·Journal of ethnopharmacology·A K Agarwal, D M Tripathi, R Sahai, et al

Management of giardiasis by a herbal drug 'Pippali Rasayana': a clinical study.

RCTn = 50Gut Health

RCT of Pippali Rasayana, an ayurvedic herbal drug, in 50 patients with giardiasis. After 15 days, 23 out of 25 treated patients showed complete disappearance of Giardia lamblia from stools, with significant reduction in symptoms and improvement in clinical and haematological profiles.

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2026·Chirality·Guanghui Gou, Wenli Bao, Liu Liu, et al

Isolation and Identification of Bioactive Fatty Acid Amides From Piper longum Fruits.

In vitroInflammation

The study isolated and identified bioactive fatty acid amides from Piper longum fruits, revealing significant anti-inflammatory activity for compounds (+)-4 and (+)-7, and potent cytotoxic effects against HT-29 cells for compound 10. These findings suggest promising anti-inflammatory and cytotoxic properties of fatty acid amides from P. longum.

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2025·Fitoterapia·Ting Li, Xin-Yuan Yu, Xin-Yi Chen, et al

Four new piperidine amide alkaloids from Piper longum fruits and their anti-inflammatory activities.

In vitroInflammation

Phytochemical investigation of Piper longum fruits led to the isolation of 20 piperidine amide alkaloids, including 4 new ones. Compounds 2, 3, 16, and 17 showed significant anti-inflammatory activity by inhibiting NO secretion in LPS-stimulated RAW264.7 cells, outperforming hydrocortisone.

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2025·Fitoterapia·Guanghui Gou, Wenli Bao, Liu Liu, et al

Amide alkaloids from the fruits of Piper longum L. and their bioactivities.

In vitro

The study isolated and characterized eight new and 17 known amide alkaloids from Piper longum fruits. These compounds were tested for NO inhibition, cytotoxic, and anti-diabetic activities in vitro. Some compounds showed moderate NO inhibitory activity, significant cytotoxic effects against HeLa and HT-29 cells, and moderate inhibitory activity against α-glucosidase and PTP1B.

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2025·Phytomedicine : international journal of phytotherapy and phytopharmacology·Long Cheng, Zonghao Liu, Minghong Gan, et al

Piper longum ameliorates obesity by regulating gut microbiota and promoting adipose thermogenesis.

Animal studyPhysique Gut Health

The study investigates the anti-obesity effects of ethanol extract of Piper longum (EEPL) by regulating adipose thermogenesis and gut microbiota. EEPL activates the TRPV1/CaMK2β/AMPK pathway to promote WAT browning and BAT activation, improving obesity in mice. It also restores gut microbiota homeostasis and reduces food intake, suggesting potential for obesity treatment.

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2025·Journal of Ayurveda and integrative medicine·Dnyaneshwar U Bawankule, Amit Kaushik, Mridula Sahu, et al

In-vivo effects of IVT-15 - A traditional knowledge-inspired formulation for the management of rheumatoid arthritis.

Animal studyInflammation Joint Bone Health Immunity

The study scientifically validated the efficacy of IVT-15, a combination of Cyperus scariosus, Zingiber officinale, and Piper longum, against rheumatoid arthritis in animal models. IVT-15 demonstrated anti-arthritic efficacy by reducing arthritis scores and inflammation markers, and showed potential analgesic and immunomodulatory properties.

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2025·Fitoterapia·Jia Liu, Teng Zhao, Jing-Rong Li, et al

Eight new amide alkaloids from Piper Longum and their anti-inflammatory activities.

In vitroInflammation

The study isolated eight new amide alkaloids from Piper Longum and evaluated their anti-inflammatory activities. Compound 7 inhibited NO release and the expression of PGE and TNF-α inflammatory factors in RAW 264.7 cells, and was predicted to bind iNOS and COX-2 proteins.

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2025·Molecular nutrition & food research·Avinash Sanap, Ramesh Bhonde, Madhura Shekatkar, et al

Novel Combination of Traditional Ayurvedic Herb Piper longum L. and Modern Stem Cell Therapy for the Reversal of Glucocorticoid-Induced Osteoporosis.

Animal studyJoint Bone Health

The study investigated the effects of umbilical cord-derived mesenchymal stem cells (UCMSCs) and Piper longum L. on glucocorticoid-induced osteoporosis in mice. The combination of UCMSCs and P. longum L. extract significantly improved bone health parameters, suggesting a synergistic effect in reversing osteoporosis.

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2025·Journal of natural medicines·Guanghui Gou, Liu Liu, Wenli Bao, et al

Amide and phenylpropanoid glycosides from the fruits of Piper longum L. and their anti-inflammatory activity.

In vitroInflammation

Ten glycosidic compounds were isolated from the fruits of Piper longum L., including two novel amide glycosides and one new phenylpropanoid glycoside. Compounds 8 and 9 showed moderate inhibitory effects on nitric oxide production in LPS-stimulated RAW264.7 cells, indicating anti-inflammatory activity.

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2024·Frontiers in pharmacology·Hee Geun Jo, Chae Yun Baek, Donghwan Kim, et al

Network analysis,, andexperiments identified the mechanisms by whichL. [Piperaceae] alleviates cartilage destruction, joint inflammation, and arthritic pain.

Animal studyJoint Bone Health Inflammation

The study evaluated the efficacy of L. [Piperaceae] (PL) in inhibiting pain and joint destruction in osteoarthritis (OA) through its anti-inflammatory activity. Network analysis and molecular docking identified potential targets and pathways, while experiments showed PL inhibited pro-inflammatory factors and cartilage degradation in an OA-induced rat model.

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2024·Fitoterapia·Guanghui Gou, Nannan Xu, Hongliang Li, et al

Sesquiterpenes from the fruits of Piper longum L. and their anti-inflammatory activity.

In vitroInflammation

The study isolated thirteen sesquiterpenes from Piper longum fruits, including four new compounds. Compounds 2 and 11 showed moderate inhibitory effects on NO release in LPS-induced RAW264.7 cells, supporting the anti-inflammatory function of P. longum.

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2024·Journal of ethnopharmacology·Chen Wu, Zhongyun Zhang, Lijie Bai, et al

Piper longum L. ameliorates gout through the MAPK/PI3K-AKT pathway.

Animal studyJoint Bone Health

The study investigates the potential of alcoholic extracts from Piper longum fruit spikes as a treatment for gout. Using network pharmacology and experimental validation, the study found that Piper longum modulates the PI3K-AKT and MAPK signaling pathways to reduce acute inflammation and kidney damage in mouse models of gout.

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2026·Journal of Ayurveda and integrative medicine·Maitri J Parmar, Rutu D Shah, Kalapi B Patel, et al

Management of a chronic non-healing viper bite ulcer in a patient with alcoholic liver cirrhosis: A Case Report.

Case reportSkin Hair Health

Case report of a 50-year-old male with a chronic non-healing viper bite ulcer and alcoholic liver cirrhosis. Ayurveda management with vardhamana pippali and other therapies improved ulcer healing, liver function, and ascites over 10 months, with complete healing and no recurrence.

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2026·Drug design, development and therapy·Ankita Bhatia, Jyoti Mehta, Ahmed Raza Hashmi, et al

From Ancient Spice to Advanced Science: Therapeutic, Nutraceutical and Nanotechnological Insights into the Fruits ofLinn for Modern Drug Development.

Review

This review explores the phytochemistry, therapeutic potential, and translational relevance of Piper longum in drug development. It highlights its bioactive compounds and activities such as antimicrobial, antioxidant, anti-inflammatory, cardioprotective, antidiabetic, and anticancer. The paper also discusses its role as a bioenhancer and its applications in nutraceuticals and nanotechnology.

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2026·Journal of ethnopharmacology·Jing Huang, Chenchen Sun, Guodong Lou

Probable interaction between warfarin and a toothache solution containing Piper longum L. leading to ischemic stroke: a case report.

Case report

Case report of a probable interaction between warfarin and a toothache solution containing Piper longum, leading to ischemic stroke. Fluctuations in INR were observed in a patient with a mechanical heart valve taking warfarin, suggesting a potential interaction.

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2022·Phytotherapy Research·P. Biswas, Mimosa Ghorai, Tulika Mishra, et al

Piper longum L.: A comprehensive review on traditional uses, phytochemistry, pharmacology, and health‐promoting activities

Review

This narrative review covers the traditional uses, phytochemistry, pharmacology, and health-promoting activities of Piper longum. It discusses its use in traditional medicine for conditions like bronchitis and snakebites, and its bioactive compounds with various pharmacological properties such as antimicrobial, anti-inflammatory, and antioxidant effects. The review also addresses safety, toxicity, and clinical trials, highlighting research gaps and future perspectives.

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2024·Journal of Ayurveda and integrative medicine·Jagruti R Parmar, Naiya Upadhyay, Manish V Patel, et al

Ayurvedic management of systemic sclerosis - A case report.

Case reportSkin Hair Health Mental Clarity

Case report of a 45-year-old woman with diffuse systemic sclerosis treated with Ayurvedic management including Piper longum and other herbal formulations. After 8 weeks, improvements were noted in skin depigmentation, hair growth, skin softening, and mental well-being.

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2025·Journal of Ayurveda and integrative medicine·Manisha Chaudhary, Mandip Goyal, Charmi Mehta

Ayurvedic management of Budd Chiari Syndrome - A case report.

Case reportn = 1

Case report of a 51-year-old female with Budd-Chiari Syndrome treated with Ayurvedic management including Deepana, Pachana, Koshthashodhana, Vardhamana pippalirasayana, and Shamana chikitsa. After three months, liver profile improved significantly and quality of life was enhanced.

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