Research

Pine Bark Extract

31 peer-reviewed studies curated from PubMed and Semantic Scholar.

2
Meta-analyses
0
Systematic reviews
11
RCTs
18
Other studies
Meta-analyses (6%)
RCTs (35%)

Studies

Sorted by quality and recency

2025·BMC complementary medicine and therapies·Shooka Mohammadi, Tamas Fulop, Abdelouahed Khalil, et al

Does supplementation with pine bark extract improve cardiometabolic risk factors? A systematic review and meta-analysis.

Meta-analysisn = 1,685Heart Health Inflammation Nutrition

Systematic review and meta-analysis of 27 RCTs with 1,685 participants evaluating the effects of pine bark extract supplementation on cardiometabolic risk factors. PBE significantly reduced systolic and diastolic blood pressure, fasting blood sugar, hemoglobin A1c, body weight, and LDL cholesterol, but had no significant impact on other measured parameters.

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2020·The Cochrane database of systematic reviews·Nina U Robertson, Anel Schoonees, Amanda Brand, et al

Pine bark (Pinus spp.) extract for treating chronic disorders.

Meta-analysisn = 1,641

This meta-analysis reviewed 27 RCTs with 1641 participants to assess the efficacy and safety of pine bark extract supplements for treating various chronic disorders. The review found very low-certainty evidence for the effects of pine bark extract on conditions such as asthma, ADHD, cardiovascular disease, diabetes, erectile dysfunction, and others, with no definitive conclusions on efficacy or safety due to small sample sizes and poor reporting.

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2021·Osteoarthritis and cartilage·X Liu, S Robbins, J Eyles, et al

Efficacy and safety of a supplement combination on hand pain among people with symptomatic hand osteoarthritis an internet-based, randomised clinical trial the RADIANT study.

RCTn = 106Joint Bone Health -

The RADIANT study was an internet-based, double-blind, randomised, placebo-controlled trial investigating the efficacy and safety of a supplement combination (Boswellia serrata, pine bark extract, methylsulfonylmethane, and curcumin) in people with hand osteoarthritis. The study found no significant difference in hand pain reduction between the supplement and placebo groups over 12 weeks.

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2021·Phytotherapy research : PTR·Cheng-Dien Hsu, Li-Hsuan Hsieh, Ya-Ling Chen, et al

Complementary effects of pine bark extract supplementation on inattention, impulsivity, and antioxidative status in children with attention-deficit hyperactivity disorder: A double-blinded randomized placebo-controlled cross-over study.

RCTn = 20Focus Mental Clarity Inflammation

This double-blind, randomized, placebo-controlled crossover study investigated the effects of pine bark extract supplementation on inattention, impulsivity, and antioxidative status in children with ADHD. Pine bark extract significantly reduced inattention and hyperactivity-impulsivity symptoms and improved antioxidative status, though no correlation was found between cognitive improvements and antioxidative markers.

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2020·Complementary therapies in medicine·S. Heffernan, C. McCarthy, C. McCarthy, et al

Mineral rich algae with pine bark improved pain, physical function and analgesic use in mild-knee joint osteoarthritis, compared to Glucosamine: A randomized controlled pilot trial.

RCTn = 30Joint Bone Health Physical Performance

A double-blinded crossover pilot trial with 30 participants compared the effects of Lithothamnion species, seawater-derived magnesium, and pine bark (Aq+) to glucosamine on knee osteoarthritis symptoms. Aq+ improved pain, physical function, and reduced analgesic use significantly more than glucosamine, suggesting it as a potential supplementary treatment for early-stage knee osteoarthritis.

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2014·Journal of women's health care·R. Stanislavov, P. Rohdewald

PACR (Pine Bark Extract, L Arginine, L Citrulline, Rose Hip Extract)Improves Emotional, Physical Health and Sexual Function in Peri-Menopausal Women

RCTn = 80Sexual Health Womens Health

Double-blind, placebo-controlled study of 80 peri-menopausal women supplemented with PACR (Pine Bark Extract, L-Arginine, L-Citrulline, Rose Hip Extract). PACR improved sexual function by 60% after 1 month and 73% after 2 months, and relieved climacteric symptoms compared to placebo. It also positively influenced metabolic parameters and antioxidant capacity.

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2012·Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association·Mathias A E Frevel, Andrew Pipingas, Warren J Grigsby, et al

Production, composition and toxicology studies of Enzogenol® Pinus radiata bark extract.

RCT

The paper presents production, composition, and safety data of Enzogenol, a pine bark extract, from rat, dog, and human studies. Toxicological studies showed no mutagenic activity and no adverse effects on body weight, blood chemistry, or organ health in animals. Human studies with doses of 480 mg/day for 6 months and 960 mg/day for 5 weeks showed no adverse effects on liver and kidney function or haematology, supporting its safe use as a food ingredient.

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2010·Archives of internal medicine·Rebecca L Drieling, Christopher D Gardner, Jun Ma, et al

No beneficial effects of pine bark extract on cardiovascular disease risk factors.

RCTn = 130Heart Health -

RCT of 130 individuals with increased cardiovascular disease risk comparing 200 mg pine bark extract to placebo over 12 weeks. The study found no significant differences in blood pressure or other cardiovascular risk factors between the groups.

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2010·BJU international·Andrea Ledda, Gianni Belcaro, Maria R Cesarone, et al

Investigation of a complex plant extract for mild to moderate erectile dysfunction in a randomized, double-blind, placebo-controlled, parallel-arm study.

RCTn = 124Sexual Health

This double-blind, placebo-controlled study assessed the effects of Prelox, a formulation of pine bark extract and l-arginine aspartate, on erectile dysfunction in 124 men aged 30-50 years over 6 months. The study found significant improvements in erectile function and plasma testosterone levels with Prelox compared to placebo.

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2023·Journal of pharmaceutical and biomedical analysis·Jasmin Bayer, Petra Högger

Development and validation of a LC-MS/MS method for the quantification of phenolic compounds in human saliva after intake of a procyanidin-rich pine bark extract.

Study

The study developed and validated an LC-MS/MS method for quantifying phenolic compounds in human saliva after intake of procyanidin-rich pine bark extract. The method was applied to a saliva sample from a volunteer, detecting various polyphenols and the gut microbial metabolite M1, suggesting enzymatic deconjugation during transfer from serum to saliva.

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2021·Foods·P. Ferreira-Santos, R. Ibarz, J. Fernandes, et al

Encapsulated Pine Bark Polyphenolic Extract during Gastrointestinal Digestion: Bioaccessibility, Bioactivity and Oxidative Stress Prevention

StudyInflammation Immunity

The study encapsulated pine bark extract rich in phenolic compounds using spray-drying with maltodextrin to enhance antioxidant and antimicrobial activity during gastrointestinal digestion. The encapsulation improved bioaccessibility and protected the compounds against oxidative stress and acidic conditions, suggesting potential applications in food formulations and nutraceuticals.

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2019·Journal of dietary supplements·Randy L Aldret, David Bellar

A Double-Blind, Cross-Over Study to Examine the Effects of Maritime Pine Extract on Exercise Performance and Postexercise Inflammation, Oxidative Stress, Muscle Soreness, and Damage.

RCTn = 20Physical Performance Inflammation

Double-blind, placebo-controlled, cross-over study of 20 young males examining the effects of 14 days of maritime pine extract supplementation on exercise performance and postexercise inflammation, oxidative stress, and muscle soreness. Maritime pine extract significantly reduced oxidative stress compared to placebo.

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2019·The Journal of pharmacy and pharmacology·Xiaoqian Liu, David J Hunter, Jillian Eyles, et al

Pharmacokinetic assessment of constituents of Boswellia serrata, pine bark extracts, curcumin in combination including methylsulfonylmethane in healthy volunteers.

RCTn = 16

This study investigated the pharmacokinetics of Boswellia serrata, pine bark extract, curcumin, and methylsulfonylmethane in healthy volunteers. It was a randomized, open-label, single-dose, four-treatment, four-period, crossover study with 16 participants. The study found no significant pharmacokinetic interactions among the constituents when administered in combination compared to alone.

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2018·Journal of nutritional science and vitaminology·Kiyoko Watanabe, Hiroko Hiramine, Toshizo Toyama, et al

Effects of French Pine Bark Extract Chewing Gum on Oral Malodor and Salivary Bacteria.

RCTn = 21

RCT investigating the effects of French pine bark extract (Pycnogenol) chewing gum on oral malodor and salivary bacteria in 21 adults. PYC gum significantly reduced exhaled hydrogen sulfide, methyl mercaptan, and dimethyl sulfide after 2 weeks, and lowered tongue-coating score and hydrogen sulfide-producing bacteria in saliva after 4 weeks compared to placebo.

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2003·Phytotherapy research : PTR·Brett Shand, Chris Strey, Russell Scott, et al

Pilot study on the clinical effects of dietary supplementation with Enzogenol, a flavonoid extract of pine bark and vitamin C.

RCTn = 24Heart Health Physique

A 12-week pilot study on dietary supplementation with Enzogenol, a pine bark extract, and vitamin C in 24 healthy subjects aged 55-75. The study found significant reductions in body weight, body fat percentage, systolic blood pressure, and plasma viscosity, with increased blood flow in forearm resistance vessels, indicating potential cardiovascular benefits.

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2024·Frontiers in Nutrition·T. Sánchez-Moya, R. López-Nicolás, P. Peso-Echarri, et al

Effect of pine bark extract and its phenolic compounds on selected pathogenic and probiotic bacterial strains

In vitroGut Health

In vitro study investigating the antimicrobial activity of French maritime pine bark extract (PBE) and its phenolic constituents on pathogenic and probiotic bacterial strains. PBE and phenolic compounds showed significant inhibitory effects on pathogens, while also affecting probiotic bacteria, with mixed outcomes on gut health.

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2022·Animals : an Open Access Journal from MDPI·N. Vera, C. Gutiérrez-Gómez, Pamela Williams, et al

Comparing the Effects of a Pine (Pinus radiata D. Don) Bark Extract with a Quebracho (Schinopsis balansae Engl.) Extract on Methane Production and In Vitro Rumen Fermentation Parameters

In vitro

The study compared the effects of pine bark extract (PBE) and quebracho extract (QTE) on methane production and in vitro rumen fermentation parameters. Both extracts decreased methane, total volatile fatty acids, and ammonia nitrogen production, while increasing acetate proportion. QTE increased propionate proportion but decreased dry matter disappearance. PBE could be used as an additive in ruminant diets to reduce methane and ammonia nitrogen production.

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2021·Journal of Cosmetic Dermatology·E. Ayres, J. Silva, S. Eberlin, et al

Invitro effect of pine bark extract on melanin synthesis, tyrosinase activity, production of endothelin‐1, and PPAR in cultured melanocytes exposed to Ultraviolet, Infrared, and Visible light radiation

In vitroSkin Hair Health

In vitro study of French maritime pine bark extract (Pycnogenol) on melanin synthesis, tyrosinase activity, endothelin‐1 production, and PPAR in cultured melanocytes exposed to various light radiations. The study explores its depigmenting action due to antioxidant, anti‐inflammatory, and anti‐melanogenic activity.

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2021·Parasitology Research·B. Blomstrand, H. Enemark, Ø. Øines, et al

Extracts of pine bark (Pinus sylvestris) inhibit Cryptosporidium parvum growth in cell culture

In vitroGut Health

In vitro study examining the anti-cryptosporidial properties of Scots pine bark extract against Cryptosporidium parvum. Acetone and methanol extracts showed dose-dependent inhibition of parasite growth, with similar IC50 values for both extracts.

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2020·Journal of Alzheimer's Disease·K. Ono, Daisy Zhao, Qingli Wu, et al

Pine Bark Polyphenolic Extract Attenuates Amyloid-β and Tau Misfolding in a Model System of Alzheimer’s Disease Neuropathology

In vitroBrain Health

The study investigates the effects of oligopin, a pine bark extract, on amyloid-β and tau misfolding in Alzheimer's disease models. Oligopin inhibited oligomer formation of Aβ1-40, Aβ1-42, and tau in vitro, and pharmacokinetics analysis identified metabolites in the plasma of mice.

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2020·Current pharmaceutical design·W. Dridi, N. Bordenave

Pine bark phenolic extract: current uses and potential food applications.

Review

Narrative review summarizing research on Pine Bark Phenolic Extracts (PBPE), including their origin, extraction methods, composition, health effects, and incorporation into food products. PBPE are rich in procyanidins and have potential health effects on cancer, diabetes, eye, and skin health. Challenges exist in developing commercially suitable extraction methods and incorporating PBPE into food products.

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2020·Definitions

French Maritime Pine Bark Extract

ReviewImmunity Inflammation Heart Health

The paper discusses the potential immunomodulating and antioxidant activities of French maritime pine bark extract, which contains proanthocyanidins. It may inhibit cellular damage, ameliorate lymphedema symptoms, improve blood flow, stimulate the immune system, and have antioxidant effects.

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2019·Cosmetics·S. Aladren, A. Garre, P. Valderas-Martínez, et al

Efficacy and Safety of an Oral Nutritional (Dietary) Supplement Containing Pinus pinaster Bark Extract and Grape Seed Extract in Combination with a High SPF Sunscreen in the Treatment of Mild-to-Moderate Melasma: A Prospective Clinical Study

Studyn = 30Skin Hair Health

Prospective clinical study evaluating an oral supplement containing Pinus pinaster and Grape seed extract, along with vitamins and minerals, used with high SPF sunscreen in 30 women with mild-to-moderate facial melasma. The study found significant improvements in melasma severity and pigmentation with no adverse events.

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2019·Journal of Industrial Textiles·P. Karakaya, Ayberk Oktay, N. Seventekin, et al

Design of a new generation wound dressing with pine bark extract

Animal studySkin Hair Health

The study designed a new wound dressing enriched with Pinus brutia extract, using microwave-assisted extraction. In vivo studies on rats showed that the extract-embedded alginate gel dressings improved wound healing rates to 75.7% compared to 48.6% in the control group.

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2018·Acta Agriculturae Scandinavica, Section A — Animal Science·N. Vera, C. Gutiérrez, R. Allende, et al

Dose–response effect of a pine bark extract on in vitro ruminal ammonia and methane formation kinetics

In vitro

In vitro study assessing the potential of pine bark extract to decrease methane and ammonia nitrogen production. The study used dietary substrates including mixed hay, soybean meal, and corn grain.

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2017·Laboratory Animal Research·Je-Won Ko, Na-Rae Shin, Sung-Hyeuk Park, et al

Pine bark extract (Pycnogenol®) suppresses cigarette smoke-induced fibrotic response via transforming growth factor-β1/Smad family member 2/3 signaling

Animal studyInflammation

The study explored the effects of Pycnogenol (pine bark extract) on pulmonary fibrosis in mice exposed to cigarette smoke and lipopolysaccharide. Pycnogenol reduced inflammatory cells, proinflammatory mediators, and collagen deposition in lung tissue, suggesting its potential as a therapeutic strategy for controlling COPD progression.

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2017·Renal Failure·In-Chul Lee, Je-Won Ko, Sung-Hyeuk Park, et al

Ameliorative effects of pine bark extract on cisplatin-induced acute kidney injury in rats

Animal studyInflammation

Animal study investigating the effects of pine bark extract (PBE) on cisplatin-induced acute kidney injury in rats. PBE treatment lowered serum blood urea nitrogen and creatinine levels, attenuated histopathological and apoptotic changes, suppressed lipid peroxidation, prevented depletion of glutathione, and enhanced antioxidant enzyme activities in kidney tissue.

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2011·Evidence-based Complementary and Alternative Medicine : eCAM·T. Shimada, Mitsutaka Kosugi, D. Tokuhara, et al

Preventive Effect of Pine Bark Extract (Flavangenol) on Metabolic Disease in Western Diet-Loaded Tsumura Suzuki Obese Diabetes Mice

Animal studyInflammation Nutrition Physique Hormone Health

The study investigated the preventive effects of Pine bark extract (Flavangenol) on metabolic disease in Tsumura Suzuki Obese Diabetes mice fed a Western diet. Flavangenol suppressed weight gain, fat accumulation, and symptoms related to insulin resistance, abnormal glucose/lipid metabolism, and hypertension. It increased adiponectin and decreased TNF-α levels, suggesting a preventive effect on metabolic disease.

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2006·Headache·Sirichai Chayasirisobhon

Use of a pine bark extract and antioxidant vitamin combination product as therapy for migraine in patients refractory to pharmacologic medication.

Studyn = 12Brain Health

Uncontrolled preliminary study of 12 patients with migraine refractory to pharmacologic medication, treated with a combination of pine bark extract, vitamin C, and vitamin E for 3 months. Significant improvements were observed in migraine disability assessment scores, headache days, and headache severity.

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2019·Trends in Food Science & Technology·Inés Mármol, Javier Quero, Nerea Jiménez-Moreno, et al

A systematic review of the potential uses of pine bark in food industry and health care

Review

This narrative review highlights the potential of pine bark extract as a source of antioxidants and its use in treating various diseases. Pine bark contains bioactive molecules like polymeric flavanols and flavonoids. It has been used in the food industry as an antioxidant and tested against disorders such as cancer, diabetes, and hypertension.

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2014·Laboratory Animal Research·Je-Won Ko, In-Chul Lee, Sung-Hyuk Park, et al

Protective effects of pine bark extract against cisplatin-induced hepatotoxicity and oxidative stress in rats

Animal studyn = 24Inflammation

Animal study investigating the protective effects of pine bark extract (pycnogenol) against cisplatin-induced hepatotoxicity and oxidative stress in rats. Pine bark extract prevented hepatotoxicity and increased antioxidant activity in a dose-dependent manner.

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