Research
NMN (Nicotinamide Mononucleotide)
169 peer-reviewed studies curated from PubMed and Semantic Scholar.
Studies
Sorted by quality and recency
NMN supplementation as a strategy to improve oocyte quality: a systematic review and transcriptomic analysis.
Systematic review and transcriptomic analysis of NMN supplementation's effects on oocyte quality. NMN improves mitochondrial regulation, reduces oxidative stress, and modulates apoptotic and inflammatory pathways in animal models. Human transcriptomic data highlight mitochondrial and oxidative pathways as key regulatory points during oocyte maturation.
Efficacy of oral nicotinamide mononucleotide supplementation on glucose and lipid metabolism for adults: a systematic review with meta-analysis on randomized controlled trials.
Systematic review and meta-analysis of 12 RCTs with 513 participants assessing NMN supplementation on metabolic health markers. NMN significantly elevated blood NAD levels, but most clinically relevant outcomes showed no significant differences between NMN and control groups.
Improved Physical Performance Parameters in Patients Taking Nicotinamide Mononucleotide (NMN): A Systematic Review of Randomized Control Trials
Systematic review of randomized controlled trials on NMN supplementation, including 10 studies with 437 patients. NMN dosages ranged from 150 to 1200 mg/day. Patients demonstrated non-significantly improved physical performance parameters, with no serious adverse effects observed.
Effects of Nicotinamide Mononucleotide on Glucose and Lipid Metabolism in Adults: A Systematic Review and Meta-analysis of Randomised Controlled Trials
Systematic review and meta-analysis of 8 RCTs involving 342 middle-age/older adults assessing the effects of nicotinamide mononucleotide (NMN) on glucose and lipid metabolism. The analysis found no significant benefit of NMN on fasting glucose, fasting insulin, glycated hemoglobin, insulin resistance, or lipid profile.
Oral nicotinamide mononucleotide (NMN) to treat chronic insomnia: protocol for the multicenter, randomized, double-blinded, placebo-controlled trial
This is a protocol for a multicenter, randomized, double-blinded, placebo-controlled trial to assess the efficacy of oral nicotinamide mononucleotide (NMN) supplementation in treating chronic insomnia. The trial will involve 400 patients with chronic insomnia, comparing NMN 320 mg/day to placebo over 60 days, with primary and secondary outcomes focused on sleep quality measures.
Towards personalized nicotinamide mononucleotide supplementation: nicotinamide adenine dinucleotide concentration
Post-hoc analysis of a double-blinded clinical trial with 80 adults aged 40-65 years, testing NMN supplementation at doses of 300 mg, 600 mg, or 900 mg daily for 60 days. NMN supplementation led to a dose-dependent increase in blood NAD concentration, which was associated with improvements in the 6-minute walk test and SF-36 score, suggesting potential benefits for physical performance and longevity.
Effect of 12-Week Intake of Nicotinamide Mononucleotide on Sleep Quality, Fatigue, and Physical Performance in Older Japanese Adults: A Randomized, Double-Blind Placebo-Controlled Study.
This randomized, double-blind placebo-controlled study evaluated the effects of nicotinamide mononucleotide (NMN) on sleep quality, fatigue, and physical performance in 108 older Japanese adults. NMN intake in the afternoon improved lower limb function and reduced drowsiness, suggesting its potential in preventing loss of physical performance and improving fatigue.
The efficacy and safety of β-nicotinamide mononucleotide (NMN) supplementation in healthy middle-aged adults: a randomized, multicenter, double-blind, placebo-controlled, parallel-group, dose-dependent clinical trial.
This randomized, multicenter, double-blind, placebo-controlled trial evaluated the effects of NMN supplementation in 80 healthy middle-aged adults over 60 days. NMN increased blood NAD concentrations and improved physical performance, with the highest efficacy at 600 mg daily. NMN was safe and well tolerated, with no significant safety issues reported.
Chronic nicotinamide mononucleotide supplementation elevates blood nicotinamide adenine dinucleotide levels and alters muscle function in healthy older men
RCT of chronic oral NMN supplementation in healthy older men, showing significant increases in blood NAD+ levels and nominal improvements in gait speed and grip performance. NMN was well tolerated with no significant effect on body composition.
Nicotinamide mononucleotide increases muscle insulin sensitivity in prediabetic women.
A 10-week, randomized, placebo-controlled, double-blind trial evaluated NMN supplementation in postmenopausal women with prediabetes who were overweight or obese. NMN increased muscle insulin sensitivity, insulin signaling, and remodeling, as assessed by hyperinsulinemic-euglycemic clamp and skeletal muscle insulin signaling.
Response to Comment on “Nicotinamide mononucleotide increases muscle insulin sensitivity in prediabetic women”
Response to a comment on a study showing that nicotinamide mononucleotide (NMN) improved muscle insulin sensitivity in prediabetic women compared to placebo. Baseline differences in intrahepatic triglyceride content do not affect the observed effects of NMN on muscle.
Supplements to treat prediabetes
RCT showing that NMN supplementation promotes NAD+ metabolism and improves skeletal muscle insulin sensitivity in postmenopausal prediabetic women who are overweight or obese. NMN may be a viable therapeutic strategy to improve metabolic health during obesity.
Chronic nicotinamide mononucleotide supplementation elevates blood nicotinamide adenine dinucleotide levels and alters muscle function in healthy older men
RCT of chronic oral supplementation of 250 mg NMN per day in healthy older men for 6 or 12 weeks. NMN supplementation significantly increased blood NAD+ levels and showed nominal improvements in gait speed and grip performance, suggesting potential benefits for aging-related muscle dysfunctions.
Effect of Nicotinamide Mononucleotide on Retinal Thickness of Older Patients With Diabetes Mellitus: A Placebo-Controlled, Double-Blind Study.
This placebo-controlled, double-blind study evaluated the effect of oral nicotinamide mononucleotide (NMN) on retinal thickness in older male patients with type 2 diabetes. The study found significant differences in retinal thickness changes between the NMN and placebo groups in the temporal subretinal field of the outer circle, suggesting NMN may mitigate age-related retinal alterations.
Anti-inflammatory effects of nicotinamide mononucleotide (NMN) in human skeletal muscle after BFR-exercise.
RCT with 11 untrained men examining NMN supplementation's effects on inflammation after BFR-exercise. NMN suppressed exercise-induced increases in inflammatory markers TNF-α and IL-10 mRNA but delayed myogenic differentiation and mitochondrial replenishment, suggesting mixed effects on inflammation and negative impact on muscle recovery.
Effect of Nicotinamide Mononucleotide Concentration in Human Milk on Neurodevelopmental Outcome: The Tohoku Medical Megabank Project Birth and Three-Generation Cohort Study
Cohort study of 150 mother-child pairs examining the relationship between nicotinamide mononucleotide (NMN) concentration in breast milk and infant neurodevelopmental outcomes. NMN was the major NAD precursor in breast milk and showed a significant positive correlation with neurodevelopmental outcomes at 24 months.
Nicotinamide adenine dinucleotide metabolism and arterial stiffness after long-term nicotinamide mononucleotide supplementation: a randomized, double-blind, placebo-controlled trial
A 12-week randomized, double-blind, placebo-controlled trial investigated the effects of 250 mg/day NMN supplementation on NAD^+ metabolism and arterial stiffness in 36 healthy middle-aged adults. NMN significantly increased serum nicotinamide levels and showed potential in alleviating arterial stiffness, though no significant difference was found between groups.
MIB-626, an Oral Formulation of a Microcrystalline Unique Polymorph of β-Nicotinamide Mononucleotide, Increases Circulating Nicotinamide Adenine Dinucleotide and its Metabolome in Middle-Aged and Older Adults.
Double-blind, placebo-controlled study of MIB-626, a microcrystalline unique polymorph of NMN, in 32 overweight or obese adults aged 55-80. MIB-626 was well tolerated and significantly increased blood NMN and NAD levels compared to placebo, with dose-related effects observed.
Safety evaluation of β-nicotinamide mononucleotide oral administration in healthy adult men and women.
A randomized, double-blind, placebo-controlled study evaluated the safety of 1250 mg of β-NMN administered orally once daily for up to 4 weeks in 31 healthy adults. The study found that β-NMN is safe and well-tolerated, with no severe adverse events observed.
Oral Administration of Nicotinamide Mononucleotide Is Safe and Efficiently Increases Blood Nicotinamide Adenine Dinucleotide Levels in Healthy Subjects
Placebo-controlled, randomized, double-blind trial of 30 healthy subjects receiving 250 mg/day of NMN or placebo for 12 weeks. NMN supplementation significantly increased NAD+ levels in whole blood without causing adverse effects, suggesting it is a safe and effective strategy to boost NAD+ levels in humans.
Effects of nicotinamide mononucleotide on older patients with diabetes and impaired physical performance: A prospective, placebo-controlled, double-blind study.
A 24-week placebo-controlled, double-blinded study on older male patients with diabetes and impaired physical performance assessed the safety and efficacy of 250 mg/day oral nicotinamide mononucleotide (NMN) supplementation. NMN was tolerable without severe adverse events, but did not significantly improve grip strength or walking speed compared to placebo.
Nicotinamide mononucleotide supplementation enhances aerobic capacity in amateur runners: a randomized, double-blind study
A six-week randomized, double-blind, placebo-controlled trial with 48 amateur runners studied the effects of nicotinamide mononucleotide (NMN) supplementation on aerobic capacity. The medium and high dosage NMN groups showed increased oxygen uptake and ventilatory threshold power compared to the control group, suggesting NMN enhances aerobic capacity during exercise.
High-Level Production of Nicotinamide Mononucleotide by Engineered Escherichia Coli.
The study focuses on the high-level production of nicotinamide mononucleotide (NMN) using engineered Escherichia coli. By constructing an NMN synthesis pathway and optimizing gene expression, the researchers achieved the highest reported NMN yield, showing potential for sustainable industrial production.
NAD+-boosting agent nicotinamide mononucleotide potently improves mitochondria stress response in Alzheimer’s disease via ATF4-dependent mitochondrial UPR
The study investigates the effects of nicotinamide mononucleotide (NMN) on mitochondrial stress response in Alzheimer's disease models. NMN supplementation improves mitochondrial proteostasis, decreases hippocampal synaptic disruption, neuronal loss, and brain atrophy in mice models of AD, leading to transcriptional changes in astrocytes and improved memory performance.
Nicotinamide Mononucleotide (NMN) Works in Type 2 Diabetes through Unexpected Effects in Adipose Tissue, Not by Mitochondrial Biogenesis
The study investigates the effects of nicotinamide mononucleotide (NMN) on glucose uptake in type 2 diabetes, revealing organ-specific effects in adipose tissue, muscle, liver, and brain. NMN enhances glucose conversion to heat in adipose tissue and shows a repressive effect on mitochondrial biogenesis in muscle and brain, with metabolic rewiring observed in the brain.
Mechanisms Underlying the Therapeutic Effects of Nicotinamide Mononucleotide in Treating High-Fat Diet-induced Hypertrophic Cardiomyopathy based on GEO Datasets, Network Pharmacology, and Molecular Docking.
The study explores the effects and mechanisms of nicotinamide mononucleotide (NMN) on high-fat diet-induced hypertrophic cardiomyopathy (HCM) using network pharmacology and molecular docking. It identifies potential targets and pathways, suggesting NMN's effects are mediated by genes related to inflammation, apoptosis, and oxidative stress, as well as the FOXO and PI3K-Akt signaling pathways.
NAD+ exhaustion by CD38 upregulation contributes to blood pressure elevation and vascular damage in hypertension
The study investigates the role of NAD+ levels in hypertension, showing that NAD+ boosting therapy with nicotinamide mononucleotide (NMN) reduces blood pressure and ameliorates vascular dysfunction in hypertensive patients and mice. CD38 upregulation leads to NAD+ exhaustion, contributing to blood pressure elevation and vascular damage.
Cinnamomum verum J. Presl Bark Contains High Contents of Nicotinamide Mononucleotide
The study identified that the bark of Cinnamomum verum J. Presl contains high levels of nicotinamide mononucleotide (NMN), an important precursor of NAD+. The research highlights the potential of C. verum in developing natural plant-based formulations for anti-aging and prevention of aging-related diseases.
Enzymatic synthesis of high-titer nicotinamide mononucleotide with a new nicotinamide riboside kinase and an efficient ATP regeneration system
The paper describes the enzymatic synthesis of nicotinamide mononucleotide (NMN) using a new nicotinamide riboside kinase from Kluyveromyces marxianus. The study highlights the high catalytic efficiency and stability of the enzyme, achieving a high yield of NMN production, indicating its potential for industrial-scale bio-manufacturing.
Small extracellular vesicles derived from mesenchymal stromal cells loaded with β-nicotinamide mononucleotide activate NAD+/SIRT3 signaling pathway-mediated mitochondrial autophagy to delay skin aging
The study investigates the effects of β-nicotinamide mononucleotide (NMN) loaded into small extracellular vesicles derived from mesenchymal stromal cells on skin aging. NMN-sEVs were shown to improve skin aging in mice, delay cellular senescence, and restore mitochondrial dysfunction by promoting NAD+/SIRT3 pathway-mediated mitophagy.
Administration of nicotinamide mononucleotide suppresses the progression of age-related hearing loss in mice.
Animal study investigating the effect of nicotinamide mononucleotide (NMN) on age-related hearing loss (ARHL) in C57BL/6J mice. Oral administration of NMN at 500 mg/kg/day suppressed the development of ARHL, increased NAD+ levels in inner ear tissues, and induced changes in genes related to metal ion metabolism.
Nicotinamide Mononucleotide (NMN) Improves the Senescence of Mouse Vascular Smooth Muscle Cells Induced by Ang II Through Activating p-AMPK/KLF4 Pathway
The study investigates the effects of nicotinamide mononucleotide (NMN) on mouse vascular smooth muscle cells (VSMCs) senescence induced by angiotensin II (Ang II). NMN reduced senescence markers and reversed Ang II-induced VSMCs senescence by activating the AMPK/KLF4/p16 pathway, suggesting its potential as a supplement for preventing vascular senescence.
pH-Responsive Transdermal Release from Poly(vinyl alcohol)-Coated Liposomes and Transethosomes: Investigating the Role of Coating in Delayed Drug Delivery.
The study develops NMN-loaded liposomes and transethosomes coated with poly(vinyl alcohol) for stable, pH-responsive transdermal delivery, improving bioavailability. PVA coating enhances colloidal stability and entrapment efficiency, with ex-vivo studies showing delayed, pH-dependent NMN release.
Effects of Time-Restricted Fasting–Nicotinamide Mononucleotide Combination on Exercise Capacity via Mitochondrial Activation and Gut Microbiota Modulation
The study investigated the effects of time-restricted fasting combined with nicotinamide mononucleotide (NMN) on exercise capacity in Kunming mice. Results showed improved mitochondrial function, enhanced endurance, limb strength, motor coordination, and balance, as well as increased gut microbiota diversity and short-chain fatty acids, leading to improved exercise performance.
NAD+ modulation with nicotinamide mononucleotide coated 3D printed microneedle implants.
The study investigates a non-invasive optical technique to upregulate NAD+ in keratinocytes using nicotinamide mononucleotide (NMN) coated microneedle implants. It evaluates the effects of NMN on intracellular NADH fluorescence in vitro and ex vivo, demonstrating a promising delivery system for NAD+ precursors.
E1231/NMN protects against experimental metabolic syndrome: the central role of SIRT1 in modulating AKT/Nrf2/NFκB signaling
The study investigates the effects of E1231 and nicotinamide mononucleotide (NMN) on metabolic syndrome (MetS) through SIRT1 activation. Results showed increased SIRT1 levels and activity, improved insulin resistance, blood pressure, lipid profile, and glucose tolerance, along with enhanced liver and kidney functions. The protective effects were linked to modulating AKT/Nrf2/NFκB signaling pathways.
Administration of β-Nicotinamide Mononucleotide Attenuates Myocardial Dysfunction in Tail-Suspended Mice
Animal study investigating the effects of β-nicotinamide mononucleotide (NMN) on myocardial dysfunction in tail-suspended mice simulating microgravity. NMN administration attenuated myocardial atrophy and preserved myocardial function, associated with reduced oxidative stress and improved autophagic flux.
NMN reverses D-galactose-induced neurodegeneration and enhances the intestinal barrier of mice by activating the Sirt1 pathway
The study investigated the effects of NMN supplementation on D-galactose-induced neurodegeneration and intestinal barrier integrity in mice. NMN improved locomotor activity, spatial memory, and preserved intestinal mucosal architecture by activating the Sirt1/AMPK/PGC-1α pathway, reducing oxidative stress, inflammation, and apoptosis.
Nicotinamide mononucleotide boosts the development of bovine oocyte by enhancing mitochondrial function and reducing chromosome lagging
The study investigated the effects of nicotinamide mononucleotide (NMN) supplementation on bovine oocyte development. NMN increased intracellular NAD(H) levels, enhanced mitochondrial function, and reduced chromosome lagging, leading to improved developmental competence to the blastocyst stage after in vitro fertilization.
Nicotinamide mononucleotide biosynthesis and the F-actin cytoskeleton regulate spindle assembly and oocyte maturation quality in post-ovulatory aged porcine oocytes
The study investigates the role of nicotinamide mononucleotide (NMN) in regulating spindle assembly and oocyte maturation quality in post-ovulatory aged porcine oocytes. NMN was found to rescue maturation capability by activating NAD+ pathways, affecting spindle assembly and cortical F-actin polymerization.
β-Nicotinamide mononucleotide protects against hypervirulent Klebsiella pneumoniae bloodstream infection and liver injury
The study investigates the protective effects of β-nicotinamide mononucleotide (NMN) against hypervirulent Klebsiella pneumoniae bloodstream infection and liver injury in mice. NMN supplementation improved survival rates, reduced inflammatory responses, and inhibited NF-κB signaling pathway activation, suggesting its therapeutic potential in preventing liver injury caused by hvKP.
NMN Supplementation Inhibits Endothelial Cell ROS-Mediated Src/Pi3k/Akt Signaling Pathway to Protect High-Altitude Blood-Retinal Barrier
The study investigates the protective effect of nicotinamide mononucleotide (NMN) against high-altitude hypoxia-induced blood-retinal barrier (BRB) disruption in a mouse model. NMN intervention reduced endothelial cell apoptosis and permeability, protecting the endothelial barrier by regulating ROS levels and inhibiting the Src/Pi3k/Akt signaling pathway.
NMN-primed exosomes derived from infrapatellar fat pad mesenchymal stem cells exert synergistic anti-inflammatory and cartilage-protective effects via MERTK pathway activation in knee osteoarthritis.
The study evaluated the therapeutic potential of NMN-primed exosomes derived from infrapatellar fat pad mesenchymal stem cells in knee osteoarthritis. ExoNMN(+) improved chondrocyte viability, reduced inflammation, and preserved cartilage in a mouse model, demonstrating enhanced anti-inflammatory and regenerative effects through the GAS6-MERTK-PI3K/AKT signaling pathway.
SARM1 Exacerbates Pressure Overload‐Induced Cardiac Hypertrophy and Heart Failure by Enhancing NAD + Metabolism
The study investigates the role of SARM1 in heart failure (HF) using in vivo and in vitro models. It finds that SARM1 exacerbates HF by reducing NAD+ levels and impairing mitochondrial bioenergetics. Supplementation with nicotinamide mononucleotide (NMN) ameliorated hypertrophy in cardiomyocytes overexpressing SARM1.
NAD+ Metabolism Maintains the Homeostasis of Decidual Macrophages in Early Pregnancy
The study investigates the role of NAD+ metabolism in maintaining decidual macrophage homeostasis during early pregnancy. It finds that NAMPT deficiency in mice leads to increased embryonic absorption, while NMN replenishment ameliorates macrophage differentiation dysregulation and fetal loss, suggesting a potential therapeutic strategy for recurrent spontaneous abortion.
Nicotinamide mononucleotide protects ovarian function and oocyte developmental competence during chemotherapy
The study investigates the protective effects of nicotinamide mononucleotide (NMN) on ovarian function against cyclophosphamide-induced damage in female mice. NMN supplementation improved ovarian reserve, enhanced endocrine function, reduced ROS levels, alleviated DNA damage, and reduced apoptosis, thereby improving oocyte quality and developmental competence during chemotherapy.
Nicotinamide mononucleotide combined with PJ-34 protects microglial cells from lipopolysaccharide-induced mitochondrial impairment through NMNAT3-PARP1 axis
The study investigates the impact of nicotinamide mononucleotide (NMN) combined with PJ-34 on LPS-induced mitochondrial damage in microglial cells. NMN and PJ-34 were found to mitigate mitochondrial impairment by modulating the NMNAT3-PARP1 axis and promoting the PINK1/Parkin mediated mitophagy pathway.
Nicotinamide mononucleotide promotes female germline stem cell proliferation by activating the H4K16ac-Hmgb1-Fyn-PLD signaling pathway through epigenetic remodeling
The study investigates the role of nicotinamide mononucleotide (NMN) in promoting female germline stem cell (FGSC) proliferation. NMN enhances FGSC viability and proliferation by activating the H4K16ac-Hmgb1-Fyn-PLD signaling pathway through epigenetic remodeling.
Nicotinamide Mononucleotide Reduces Iron in BV-2 Cell by Up-Regulating Ferroportin 1 via Inhibiting the IL-6/STAT3/Hepcidin Pathway
In vitro study on BV-2 microglia cells showing that nicotinamide mononucleotide (NMN) reduces iron concentration, ferritin expression, and reactive oxygen species levels, while increasing cell viability in iron-overloaded cells. NMN regulates iron transport proteins via IL6/STAT3/hepcidin and IL-1β/hepcidin pathways, suggesting potential therapeutic effects on iron-related diseases like neurodegenerative diseases.
Nicotinamide mononucleotide enhances porcine sperm quality by activating the SIRT3-SOD2/ROS pathway and promoting oxidative phosphorylation.
The study investigates the effects of nicotinamide mononucleotide (NMN) supplementation on porcine sperm quality during storage. NMN improved sperm quality by increasing NAD+ concentrations, reducing reactive oxygen species (ROS), and enhancing oxidative phosphorylation via the SIRT3-SOD2/ROS pathway. Improved reproductive outcomes were observed, including healthier piglets and reduced stillbirth rates following artificial insemination.
Histidine triad nucleotide‐binding protein 2 attenuates doxorubicin‐induced cardiotoxicity through restoring lysosomal function and promoting autophagy in mice
The study investigates the role of histidine triad nucleotide‐binding protein 2 (HINT2) in doxorubicin-induced cardiotoxicity in mice. Nicotinamide mononucleotide (NMN) supplementation restored lysosomal function in vitro, and cardiac-specific Hint2 overexpression alleviated cardiotoxicity both in vivo and in vitro, suggesting potential therapeutic strategies to reduce cardiac damage caused by doxorubicin.
A Mixture of Nicotinamide Mononucleotide, Decursin, and l-Cysteine Lowered Senescence-Associated Markers In Vitro and Was Effective Against 2,4-Dinitrochlorobenzene-Induced Atopic Dermatitis In Vivo: An Application of Network Pharmacology
The study investigated the effects of a mixture of nicotinamide mononucleotide, decursin, and l-cysteine on skin aging and atopic dermatitis using in vitro and in vivo models. Mixture B reduced oxidative stress and inflammation markers, while mixture-L and mixture-H treatments improved symptoms in a mouse model of atopic dermatitis.
Safety and efficacy of long-term nicotinamide mononucleotide supplementation on metabolism, sleep, and nicotinamide adenine dinucleotide biosynthesis in healthy, middle-aged Japanese men.
An 8-week, single-center, single-arm, open-label clinical trial investigated the effects of NMN supplementation in 11 healthy, middle-aged Japanese men. NMN was well-tolerated and increased NAD+ levels in peripheral blood mononuclear cells. It modestly attenuated postprandial hyperinsulinemia in participants with insulin oversecretion, suggesting potential benefits for metabolism and longevity.
Nicotinamide Mononucleotide improves oocyte maturation of mice with type 1 diabetes
The study examined the effects of nicotinamide mononucleotide (NMN) on oocyte maturation in mice with type 1 diabetes. NMN supplementation increased the oocyte maturation rate and improved oocyte quality by enhancing mitochondrial function, reducing ROS levels, and correcting meiotic defects.
3D Printed Microneedles for the Transdermal Delivery of NAD+ Precursor: Toward Personalization of Skin Delivery.
The study optimized 3D printing parameters for microneedles to deliver nicotinamide mononucleotide (NMN) transdermally. The NMN-coated microneedles showed significant diffusion through human skin in vitro, indicating potential for personalized skin delivery.
Nicotinamide Mononucleotide Improves Endometrial Homeostasis in a Rat Model of Polycystic Ovary Syndrome by Decreasing Insulin Resistance and Regulating the Glylytic Pathway.
The study investigates the effects of nicotinamide mononucleotide (NMN) on endometrial homeostasis in a rat model of polycystic ovary syndrome (PCOS). NMN treatment restored regular estrous cycles, reduced serum androgen and fasting insulin levels, improved endometrial morphology, and activated the PI3K/AKT pathway, suggesting improved endometrial tissue homeostasis by decreasing insulin resistance and regulating glycolysis.
Dietary NMN supplementation enhances motor and NMJ function in ALS.
The study investigated the effects of dietary nicotinamide mononucleotide (NMN) supplementation on a mouse model of ALS. NMN supplementation modestly extended lifespan, delayed motor dysfunction, and improved neuromuscular junction function and morphology in ALS mice.
Fertility protection during chemotherapy treatment by boosting the NAD(P)+ metabolome
The study tested the effect of boosting the NAD(P)+ metabolome on chemotherapy-induced ovarian failure in mice. Using nicotinamide mononucleotide (NMN) as a precursor, the study found that it protected against ovarian toxicity and female infertility without impairing chemotherapy efficacy.
Glycolysis-Mediated Activation of v-ATPase by Nicotinamide Mononucleotide Ameliorates Lipid-Induced Cardiomyopathy by Repressing the CD36-TLR4 Axis
The study investigates the effects of nicotinamide mononucleotide (NMN) supplementation on lipid-induced cardiomyopathy in rats/mice. NMN preserved endosomal acidification and prevented CD36-mediated lipid accumulation, inflammation, fibrosis, cardiac dysfunction, and insulin resistance during myocardial lipid overload.
A biocompatible polydopamine platform for targeted delivery of nicotinamide mononucleotide and boosting NAD+ levels in the brain.
The study developed a polydopamine platform for targeted delivery of nicotinamide mononucleotide (NMN) to boost NAD+ levels in the brain. The PDA-Lf-NMN system improved BBB penetration and utilization of NMN in elevating NAD+ levels, enhancing spatial cognition in old mice without inducing cellular necrosis or apoptosis.
Nicotinamide mononucleotide alleviates seizures via modulating SIRT1‐PGC‐1α mediated mitochondrial fusion and fission
The study investigates the effects of nicotinamide mononucleotide (NMN) on mitochondrial dynamics and neuronal protection in seizure models. NMN treatment reduced seizure intensity, improved learning and memory, and enhanced motor activity in PTZ-induced epileptic mice. NMN also inhibited neuronal apoptosis and improved mitochondrial energy metabolism by modulating the SIRT1-PGC-1α pathway.
Nicotinamide mononucleotide ameliorates ionizing radiation-induced spermatogenic dysfunction in mice by modulating the glycolytic pathway
The study investigates the protective effects of nicotinamide mononucleotide (NMN) against ionizing radiation-induced testicular injury and spermatogenic dysfunction in male mice. NMN supplementation restored sperm quantity, motility, and testicular volume, and modulated gene expressions related to apoptosis and glycolysis, suggesting a protective strategy against reproductive damage from ionizing radiation.
NMN synbiotics intervention modulates gut microbiota and metabolism in APP/PS1 Alzheimer's disease mouse models.
Study on APP/PS1 Alzheimer's disease mouse models investigating the effects of NMN synbiotics, a combination of β-nicotinamide mononucleotide, Lactobacillus plantarum, and lactulose, on gut microbiota and metabolism. NMN synbiotics led to restructuring of gut microbiota, normalization of metabolic composition, and reduction of amyloid plaques in the brain, suggesting potential therapeutic effects on Alzheimer's disease.
Metabolite accumulation from oral NMN supplementation drives aging-specific kidney inflammation
The study tested the effects of NMN supplementation on kidney inflammation in young and aged male mice. NMN increased genetic markers of inflammation and tubule injury in aged kidneys, suggesting age-specific inflammatory responses to NMN supplementation.
β-Nicotinamide Mononucleotide Promotes Cell Proliferation and Hair Growth by Reducing Oxidative Stress
The study investigates the effects of β-Nicotinamide mononucleotide (NMN) on hair growth and cell proliferation. NMN was found to reverse hair follicle atrophy and thinning induced by DHT in mice, and it reduced oxidative stress and inflammatory mediator release in cultured human dermal papilla cells treated with DHT.
Alleviation of hepatic insulin resistance and steatosis with NMN via improving endoplasmic reticulum-Mitochondria miscommunication in the liver of HFD mice.
The study investigates the effects of NMN on liver steatosis and insulin resistance in high-fat diet mice. NMN improved liver steatosis and insulin resistance by enhancing fat digestion, absorption, and cholesterol metabolism, while reducing mitochondrial dysfunction and ER oxidative stress.
Prophylactic nicotinamide mononucleotide (NMN) mitigates CSDS-induced depressive-like behaviors in mice via preserving of ATP level in the mPFC.
The study investigates the prophylactic effects of nicotinamide mononucleotide (NMN) on depressive-like behaviors in mice induced by chronic social defeat stress (CSDS). NMN treatment elevated NAD+ biosynthesis and extracellular ATP levels in the mPFC, preventing the development of depressive-like behavior and preserving GABAergic inhibition and neuronal excitability.
β-Nicotinamide Mononucleotide Reduces Oxidative Stress and Improves Steroidogenesis in Granulosa Cells Associated with Sheep Prolificacy via Activating AMPK Pathway
In vitro study showing that β-nicotinamide mononucleotide (NMN) supplementation increases NAD+ and ATP levels in granulosa cells, reduces oxidative stress, and improves steroidogenesis via the AMPK/mTOR pathway. NMN mitigates LPS-induced apoptosis and mitochondrial dysfunction, enhancing ovarian function.
β-Nicotinamide mononucleotide improves chilled ram sperm quality in vitro by reducing oxidative stress damage
In vitro study investigating the effect of β-nicotinamide mononucleotide (NMN) on ram sperm quality during storage at 4°C. NMN supplementation improved sperm motility, acrosome integrity, and plasma membrane integrity, and reduced oxidative stress and apoptosis, suggesting NMN's efficiency in maintaining sperm viability.
Control of NAD+ homeostasis by autophagic flux modulates mitochondrial and cardiac function
The study investigates how impaired autophagic flux affects NAD+ availability in cardiomyocytes, leading to mitochondrial dysfunction and heart failure. It shows that nicotinamide mononucleotide (NMN) supplementation or NNMT inhibition restores NAD+ levels and ameliorates cardiac and mitochondrial dysfunction in autophagy-deficient hearts.
Nicotinamide Mononucleotide Restores NAD+ Levels to Alleviate LPS-Induced Inflammation via the TLR4/NF-κB/MAPK Signaling Pathway in Mice Granulosa Cells
The study investigated the effects of nicotinamide mononucleotide (NMN) on LPS-induced inflammation in mouse granulosa cells. NMN supplementation restored NAD+ levels, reduced pro-inflammatory markers, enhanced cell viability and proliferation, reduced apoptosis, and improved steroidogenesis activity. NMN also suppressed the activation of inflammatory signaling pathways, suggesting its potential as a therapeutic agent for ovarian inflammation and related fertility disorders.
Identification of adrenergic presynaptic and postsynaptic protein locations at neuromuscular junctions, their decrease during aging, and recovery by nicotinamide mononucleotide administration
The study evaluated the effect of aging on sympathetic nerves and synaptic proteins at mouse neuromuscular junctions. Age-related declines in vesicular monoamine transporter 2 and β2-adrenergic receptors were observed, which reverted to adult levels after 1 month of oral NMN administration. NMN did not alter the expression level of the sympathetic marker tyrosine hydroxylase.
Nicotinamide mononucleotide induces autophagy and ferroptosis via AMPK/mTOR pathway in hepatocellular carcinoma
The study investigates the role of nicotinamide mononucleotide (NMN) in hepatocellular carcinoma (HCC) progression. NMN treatment increased NAD+ levels and NAD+/NADH ratio, inhibited cell proliferation, and enhanced apoptosis, autophagy, and ferroptosis in HCC cells via the AMPK/mTOR pathway. NMN also restrained tumor growth in xenograft models, suggesting its potential as a treatment for HCC.
Design and preparation of NMN nanoparticles based on protein-marine polysaccharide with increased NAD+ level in D-galactose induced aging mice model.
The study designed NMN-loaded ovalbumin and fucoidan nanoparticles to improve NMN bioaccessibility and increase NAD+ levels in a D-galactose induced aging mouse model. The NMN-loaded nanoparticles improved antioxidant enzyme activity, reduced weight and organ index loss, and enhanced cognitive performance in aging mice compared to free NMN.
Nicotinamide Mononucleotide (NMN) Ameliorates Free Fatty Acid-Induced Pancreatic β-Cell Dysfunction via the NAD+/AMPK/SIRT1/HIF-1α Pathway
In vitro study using INS-1 cells to model β-cell dysfunction induced by palmitate. Nicotinamide mononucleotide (NMN) supplementation restored the expression of proteins related to the NAD+/AMPK/SIRT1/HIF-1α pathway and improved insulin secretion, suggesting NMN's potential in alleviating β-cell dysfunction.
The Role of Nicotinamide Mononucleotide Supplementation in Psoriasis Treatment
The study explores the role of nicotinamide mononucleotide (NMN) in treating psoriasis using in vivo and in vitro models. NMN treatment attenuated epidermal proliferation, splenomegaly, and inflammatory responses in IMQ-stimulated mice, and improved mitochondrial function and reduced inflammation in keratinocyte cell lines.
Exploring the De Novo NMN Biosynthesis as an Alternative Pathway to Enhance NMN Production.
The study explores the de novo NMN biosynthesis route as an alternative pathway to enhance NMN production in Escherichia coli. Systematic engineering of E. coli resulted in a significant increase in NMN yield, with the highest production reaching 3067.98 μM after 24 hours of fermentation. The findings provide insights into the NAD+ salvage pathway and its role in energy metabolism.
Microbial creation of β‐Nicotinamide mononucleotide and its regulation of lipid metabolism in the liver of high‐fat diet mice
The study investigated the effects of β-Nicotinamide mononucleotide (NMN) on lipid metabolism in high-fat diet mice. NMN supplementation increased NAD+ levels, reduced liver injury and lipid deposition, and decreased total cholesterol, triglycerides, alanine aminotransferase, and insulin levels in serum.
Nicotinamide mononucleotide supplementation rescues mitochondrial and energy metabolism functions and ameliorates inflammatory states in the ovaries of aging mice
The study investigates the effects of nicotinamide mononucleotide (NMN) supplementation on aging mice ovaries. NMN restored ovarian NAD levels, improved oocyte quality and quantity, enhanced hormone secretion, and reduced inflammation, suggesting benefits for ovarian health and fertility in aging.
Assessing the Effects of Nicotinamide Mononucleotide Supplementation on Pulmonary Inflammation in Male Mice Subchronically Exposed to Ambient Particulate Matter
The study evaluated the effects of nicotinamide mononucleotide (NMN) supplementation on pulmonary inflammation in male mice exposed to ambient particulate matter. NMN supplementation resulted in higher NAD+ levels, reduced pulmonary inflammation, and improved immune function, characterized by fewer inflammatory cells and inhibited interleukin-17 signaling.
Nicotinamide mononucleotide (NMN) intake increases plasma NMN and insulin levels in healthy subjects.
Study on the effects of daily NMN ingestion in 11 healthy volunteers over 12 weeks. NMN intake increased plasma concentrations of NMN and NAD+, as well as postprandial serum insulin levels. No adverse symptoms were observed.
Improving lysosomal ferroptosis with NMN administration protects against heart failure
The study investigates the effects of NMN supplementation on lysosome-mediated ferroptosis in mitochondrial translation-deficient mice. NMN administration improved autophagy, reduced heart failure, and extended lifespan by enhancing lysosomal function rather than mitochondrial function.
Preparation and evaluation of ovalbumin-fucoidan nanoparticles for nicotinamide mononucleotide encapsulation with enhanced stability and anti-aging activity.
The study prepared ovalbumin-fucoidan nanoparticles to encapsulate nicotinamide mononucleotide (NMN) for enhanced stability and anti-aging activity. NMN-loaded nanoparticles improved lifespan, reproductive ability, and body length in Caenorhabitis elegans compared to free NMN, suggesting improved anti-oxidative stress and anti-aging effects.
Protective effects of nicotinamide mononucleotide on radiation-related cardiac injury
The study investigates the protective effects of nicotinamide mononucleotide (NMN) on radiation-related cardiac injury in mice. NMN supplementation before radiation exposure reduced mechanical and electrical dysfunction in the heart and partially reversed harmful effects on H9C2 cells, such as increased ROS production and reduced ATP content.
Nicotinamide Mononucleotide Ameliorates Sleep Deprivation‐Induced Gut Microbiota Dysbiosis and Restores Colonization Resistance against Intestinal Infections
The study investigates the effects of nicotinamide mononucleotide (NMN) supplementation on sleep deprivation-induced gut microbiota dysbiosis in mice. NMN restores colonization resistance against intestinal infections by reprogramming gut microbiota and metabolite profiles, particularly affecting bile acid metabolism.
Nicotinamide mononucleotide attenuates airway epithelial barrier dysfunction via inhibiting SIRT3 SUMOylation in asthma.
The study investigates the effects of nicotinamide mononucleotide (NMN) on airway epithelial barrier dysfunction in asthma. NMN supplementation alleviated airway inflammation and reduced mucus secretion in house dust mite-induced asthmatic mice, and mitigated airway epithelial barrier disruption in vitro and in vivo. The effects were mediated through inhibition of SIRT3 SUMOylation.
Nicotinamide mononucleotide restores oxidative stress‐related apoptosis of oocyte exposed to benzyl butyl phthalate in mice
The study investigated the effects of benzyl butyl phthalate (BBP) on mouse oocyte maturation and early embryo development, finding that BBP exposure disrupted mitochondrial function and induced oxidative stress-related apoptosis. Nicotinamide mononucleotide (NMN) supplementation was shown to reduce these adverse effects, suggesting NMN as an effective treatment for BBP-induced reproductive toxicity.
The identification of new roles for nicotinamide mononucleotide after spinal cord injury in mice: an RNA-seq and global gene expression study
The study investigated the effects of nicotinamide mononucleotide (NMN) supplementation on spinal cord injury (SCI) in mice. NMN restored NAD+ levels, promoted motor function recovery, and alleviated pain. RNA-seq and qRT-PCR analyses showed NMN inhibited inflammation-related signaling pathways and down-regulated inflammatory factors and chemokines.
Therapeutic Effect of Nicotinamide Mononucleotide for Hypoxic–Ischemic Brain Injury in Neonatal Mice
The study investigates the neuroprotective effects of nicotinamide mononucleotide (NMN) in a neonatal mouse model of hypoxic–ischemic brain injury. NMN treatment normalized hippocampal NAD+ and SIRT6 levels, reduced caspase-3 activity and HMGB1 release, alleviated tissue loss, and improved early developmental behavior, motor, and memory function.
Nicotinamide mononucleotide (NMN) alleviates the poly(I:C)-induced inflammatory response in human primary cell cultures
The study demonstrates that NMN alleviates the poly(I:C)-induced inflammatory response in human primary cell cultures, specifically in human pulmonary microvascular endothelial cells and human coronary artery endothelial cells. NMN exposure significantly downregulated major inflammatory mediators, suggesting a regulatory mechanism for inflammation reduction.
MALNUTRITION IN COVID-19 SURVIVORS: PREVALENCE AND RISK FACTORS
The study investigates the effect of long-term NMN administration on health, cancer burden, frailty, and lifespan in male and female mice. NMN treatment increased activity, maintained youthful gene expression, reduced frailty, and altered the microbiome. It improved metabolic health in males and increased median lifespan in females without increasing cancer burden.
Nicotinamide mononucleotide as a therapeutic agent to alleviate multi-organ failure in sepsis
The study investigates the therapeutic effects of nicotinamide mononucleotide (NMN) in septic organ failure in mice. NMN administration elevated NAD^+ levels, reduced oxidative stress, inflammation, and caspase-3 activity, and improved organ function, leading to lower mortality in septic mice.
β-Nicotinamide mononucleotide activates NAD+/SIRT1 pathway and attenuates inflammatory and oxidative responses in the hippocampus regions of septic mice
The study investigates the role of β-Nicotinamide Mononucleotide (NMN) in treating sepsis-associated encephalopathy in mice. NMN activated the NAD+/SIRT1 pathway, improving memory performance and reducing inflammatory and oxidative responses in the hippocampus of septic mice.
Host–microbiome interactions in nicotinamide mononucleotide (NMN) deamidation
The study investigates the interaction between orally delivered nicotinamide mononucleotide (NMN) and the gut microbiome, showing that NMN can undergo deamidation and incorporation in mammalian tissue via the de novo pathway. Antibiotic treatment, which reduces the gut microbiome, increased the availability of NAD+ metabolites, suggesting competition between the microbiome and host for dietary NAD+ precursors.
Nicotinamide mononucleotide alleviates endotoxin-induced acute lung injury by modulating macrophage polarization via the SIRT1/NF-κB pathway
The study investigates the effects of nicotinamide mononucleotide (NMN) on sepsis-induced acute lung injury (ALI) using cultured MH-S cells and a murine model. NMN was found to decrease apoptotic rates, improve lung pathology, reduce W/D ratio and MPO activity, increase NAD+ and ATP levels, and promote M2 macrophage polarization via the SIRT1/NF-κB pathway.
Ex Vivo Transdermal Delivery of Nicotinamide Mononucleotide Using Polyvinyl Alcohol Microneedles
The study investigates the transdermal delivery of nicotinamide mononucleotide (NMN) using polyvinyl alcohol (PVA) microneedles. It examines the effect of PVA molecular weight on microneedle structure and NMN release, achieving a 91.94% release in 18 hours with a specific PVA microneedle formulation.
β-nicotinamide mononucleotide rescues the quality of aged oocyte and improves subsequent embryo development in pigs
The study investigated the effects of nicotinamide mononucleotide (NMN) on aging porcine oocytes and subsequent embryonic development. NMN supplementation reduced reactive oxygen species levels, increased antioxidant gene expression, improved mitochondrial membrane potential, and enhanced embryonic development in senescent oocytes.
Nicotinamide mononucleotide alters body composition and ameliorates metabolic disorders induced by a high‐fat diet
The study investigated the effects of nicotinamide mononucleotide (NMN) on body composition and metabolic disorders in obese mice. NMN intervention reduced fat mass, increased lean mass, improved glucose tolerance, and alleviated adipose tissue inflammation, suggesting its potential in obesity treatment through the NAD+/SIRT6/LKB1 pathway.
High-Dosage NMN Promotes Ferroptosis to Suppress Lung Adenocarcinoma Growth through the NAM-Mediated SIRT1–AMPK–ACC Pathway
The study investigates the effects of high-dose nicotinamide mononucleotide (NMN) on lung adenocarcinoma. It shows that high-dose NMN promotes ferroptosis through NAM-mediated SIRT1–AMPK–ACC signaling, inhibiting tumor growth in vitro and in vivo. The findings suggest a potential therapeutic role for NMN in cancer treatment.
Nicotinamide mononucleotide induces lipolysis by regulating ATGL expression via the SIRT1-AMPK axis in adipocytes
The study investigates the effect of nicotinamide mononucleotide (NMN) on lipid metabolism in 3T3-L1 adipocytes and mice. NMN treatment reduced lipid accumulation and enhanced lipolysis in adipocytes, increasing ATGL expression via the SIRT1-AMPK axis. In mice on a high-fat diet, NMN decreased subcutaneous fat mass and adipocyte size, indicating site-specific effects in adipose tissue.