Research

Nigella sativa (Black Seed / Black Cumin)

129 peer-reviewed studies curated from PubMed and Semantic Scholar.

25
Meta-analyses
9
Systematic reviews
75
RCTs
20
Other studies
Meta-analyses (19%)
Systematic reviews (7%)
RCTs (58%)

Studies

Sorted by quality and recency

2026·Endocrinology, diabetes & metabolism·Vali Musazadeh, Melika Jahangir, Mahsa Mahmoudinezhad, et al

The Effect of Nigella sativa Supplementation on Cardiometabolic Health in Patients With Metabolic Diseases: A GRADE-Assessed Systematic Review and Meta-Analysis.

Meta-analysisn = 2,145Heart Health Nutrition

Meta-analysis of 31 trials with 2145 participants assessing the effect of Nigella sativa supplementation on cardiovascular risk factors in patients with metabolic diseases. Significant reductions were found in weight, BMI, systolic and diastolic blood pressure, fasting blood sugar, and HbA1c, but no effect on insulin resistance or central adiposity.

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2026·Supportive care in cancer : official journal of the Multinational Association of Supportive Care in Cancer·Daniel S Alicea, Mark Han, Zahidul Islam, et al

Systematic review of prophylactic antibacterial agents for radiation-induced oral mucositis in head and neck cancer.

Systematic review

Systematic review of prophylactic antibacterial agents for radiation-induced oral mucositis in head and neck cancer patients. Nigella sativa showed potential benefits in reducing RIOM incidence and severity compared with standard care and other antibacterial agents, though evidence remains insufficient to establish superiority.

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2025·Pharmacological research·Ali Jafari, Helia Mardani, Amir Hossein Faghfouri, et al

Does Nigella sativa supplementation improve cardiovascular disease risk factors? A comprehensive GRADE-assessed systematic review and dose-response meta-analysis of 82 randomized controlled trials.

Meta-analysisn = 5,026Heart Health Inflammation Physique Nutrition

This systematic review and meta-analysis evaluated the effects of Nigella sativa supplementation on metabolic and cardiovascular health outcomes across 82 randomized controlled trials with 5026 participants. N. sativa supplementation significantly improved various cardiovascular disease risk factors, including body composition, blood pressure, blood glucose levels, lipid profiles, and inflammatory markers.

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2025·Inflammopharmacology·Hassan Ghobadi, Elmira Mohammadzadeh, Mohammad Reza Aslani

Effects of Nigella sativa and its constituents on inflammatory markers in ovalbumin-induced asthma model: a preclinical systematic review and meta-analysis.

Meta-analysisn = 502Inflammation Immunity

This meta-analysis evaluated the effects of Nigella sativa and its constituents on inflammatory markers in an animal model of ovalbumin-induced asthma. The analysis of 18 studies with 502 animals showed that N. sativa significantly reduced levels of IL-4, IL-5, IL-13, IL-17, and IgE, but did not change IFN-γ levels, suggesting potential anti-inflammatory and antioxidant effects.

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2025·Complementary therapies in medicine·Mehdi Karimi, Samira Pirzad, Seyed Morteza Ali Pourfaraji, et al

Effects of black seed (Nigella sativa L.) on cardiometabolic indices in type 2 diabetic patients: A systematic review and meta-analysis of RCTs.

Meta-analysisHeart Health Nutrition

Systematic review and meta-analysis of 16 RCTs assessing black seed supplementation in type 2 diabetic patients. Black seed significantly reduced fasting blood glucose, hemoglobin A1c, total cholesterol, and LDL levels, suggesting potential benefits for glycemic and lipid control.

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2025·Current medicinal chemistry·Maryam Shabani, Farideh Ghavidel, Arezoo Rajabian, et al

Effect ofConsumption on Lipid Profile and Glycemic Index in Patients with Metabolic Syndrome: A Systematic Review and Meta-analysis of Randomized Controlled Trials.

Meta-analysisHeart Health Nutrition

This systematic review and meta-analysis of randomized controlled trials assessed the effect of Nigella sativa consumption on lipid profile and glycemic indices in patients with metabolic syndrome. The results showed that N. sativa intake significantly decreased total cholesterol, LDL-C, HDL-C, FBS, and HbA1c concentrations, indicating improvements in lipid profile and glycemic index.

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2024·Clinical nutrition ESPEN·Mahsa Rounagh, Vali Musazadeh, Ali Hosseininejad-Mohebati, et al

Effects of Nigella sativa supplementation on lipid profiles in adults: An updated systematic review and meta-analysis of randomized controlled trials.

Meta-analysisn = 2,278Heart Health

This systematic review and meta-analysis of 34 studies with 2278 participants examined the effects of Nigella sativa supplementation on lipid profiles. The results showed significant reductions in total cholesterol, triglycerides, and LDL-C, and an increase in HDL-C, suggesting Nigella sativa as a potential adjuvant anti-hyperlipidemic agent.

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2024·Current pharmaceutical biotechnology·Hossein Hosseini, Farideh Ghavidel, Mahdieh Aliyari, et al

Effect ofIntake on Oxidative Stress and Inflammation in Patients with Metabolic Syndrome and Related Disorders: A Systematic Review and Meta-analysis of Randomized Controlled Trials.

Meta-analysisn = 1,033Inflammation

This systematic review and meta-analysis of 16 RCTs with 1033 participants assessed the impact of Nigella sativa intake on inflammation and oxidative stress biomarkers in individuals with metabolic syndrome. Results showed significant decreases in CRP, TNF-α, IL-6, and MDA levels, and increases in SOD and TAC levels in the intervention group compared to placebo.

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2024·West African journal of medicine·S Elhariri, I Burud, N A Zulaimy, et al

Systematic Review of Randomized Controlled Trials in Uses of Nigella Sativa (Black Seed) in Metabolic Syndrome.

Systematic reviewn = 776Heart Health Nutrition

Systematic review of randomized controlled trials assessing the effects of Nigella Sativa on metabolic syndrome parameters. Significant improvements were found in lipid profile and glycaemic indices, while results for blood pressure and anthropometric indices were inconsistent. Nigella Sativa is recommended as an adjunct therapy for metabolic syndrome.

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2024·Prostaglandins & other lipid mediators·Xinyu Lan, Yongliang Xia

Alleviating effects of Nigella sativa supplements on biomarkers of inflammation and oxidative stress: Results from an umbrella meta-analysis.

Meta-analysisInflammation Longevity

Umbrella meta-analysis evaluated the effects of Nigella sativa supplementation on inflammatory and oxidative markers. N. Sativa significantly decreased serum CRP, TNF-alpha, and MDA levels, and improved TAC and SOD levels, suggesting its beneficial effects as an anti-inflammatory and antioxidant agent.

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2023·Inflammopharmacology·Zeynab Kavyani, Vali Musazadeh, Sahar Golpour-Hamedani, et al

The effect of Nigella sativa (black seed) on biomarkers of inflammation and oxidative stress: an updated systematic review and meta-analysis of randomized controlled trials.

Meta-analysisn = 1,086Inflammation Inflammation -Inflammation Inflammation Inflammation Inflammation Inflammation

This meta-analysis of 20 RCTs with 1086 participants assessed the effect of Nigella sativa supplementation on inflammatory and oxidative markers. N. sativa significantly reduced CRP, TNF-α, and MDA levels, and improved TAC, GPx, and SOD levels, but had no significant effect on IL-6 levels.

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2023·Phytotherapy research : PTR·Chia Siang Kow, Dinesh Sangarran Ramachandram, Syed Shahzad Hasan

The effect of Nigella sativa on the risk of mortality in patients with COVID-19: A systematic review and meta-analysis of randomized trials.

Meta-analysis

Systematic review and meta-analysis of randomized trials examining the effect of Nigella sativa on the risk of mortality in patients with COVID-19.

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2023·Phytotherapy research : PTR·Zeynab Kavyani, Vali Musazadeh, Ehsan Safaei, et al

Antihypertensive effects of Nigella sativa supplementation: An updated systematic review and meta-analysis of randomized controlled trials.

Meta-analysisHeart Health

This systematic review and meta-analysis of randomized controlled trials examined the effects of Nigella sativa supplementation on blood pressure in adults. The analysis found that N. sativa supplementation effectively reduced both systolic and diastolic blood pressure, suggesting its potential use in blood pressure management.

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2022·Phytotherapy research : PTR·Sohaib Ashraf, Shoaib Ashraf, Moneeb Ashraf, et al

Honey and Nigella sativa against COVID-19 in Pakistan (HNS-COVID-PK): A multicenter placebo-controlled randomized clinical trial.

RCTn = 313Immunity Inflammation

Multicenter, placebo-controlled RCT in Pakistan testing honey and Nigella sativa (HNS) on COVID-19 patients. HNS significantly reduced symptom duration, expedited viral clearance, and lowered mortality compared to placebo, with no adverse effects observed.

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2022·International journal of environmental research and public health·Khalid A Bin Abdulrahman, Abdullah Omar Bamosa, Abdullah I Bukhari, et al

The Effect of Short Treatment with Nigella Sativa on Symptoms, the Cluster of Differentiation (CD) Profile, and Inflammatory Markers in Mild COVID-19 Patients: A Randomized, Double-Blind Controlled Trial.

RCTn = 262

This randomized, double-blind controlled trial investigated the impact of different doses of Nigella sativa seeds on symptoms, the cluster of differentiation profile, and inflammatory markers in mild COVID-19 patients. The study found no significant effect of ten days of treatment with Nigella sativa on these parameters.

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2022·Drug research·Farazul Hoda, Afifa Khanam, Mehak Thareja, et al

Effect of Nigella Sativa in Improving Blood Glucose Level in T2DM: Systematic Literature Review of Randomized Control Trials.

Systematic reviewNutrition

Systematic review of randomized controlled trials evaluating the effects of Nigella Sativa on glucose and insulin profile status in patients with Type 2 Diabetes Mellitus. The review found significant improvements in the management of T2DM and its complications with Nigella Sativa intervention.

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2022·International journal of molecular sciences·Mohamad Fawzi Mahomoodally, Muhammad Zakariyyah Aumeeruddy, Lesetja J Legoabe, et al

L. and Its Active Compound Thymoquinone in the Clinical Management of Diabetes: A Systematic Review.

Systematic reviewNutrition

This systematic review analyzes clinical studies on the efficacy, safety, and mechanism of action of Nigella sativa (NS) and its compound thymoquinone (TQ) in diabetes management. The review found that NS and TQ have potent hypoglycemic activity, improving parameters such as fasting blood glucose, postprandial blood glucose, and HbA1c. TQ combined with metformin showed greater reductions in HbA1c and blood glucose compared to metformin alone.

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2021·Phytotherapy research : PTR·Gang Tang, Linyu Zhang, Jie Tao, et al

Effect of Nigella sativa in the treatment of nonalcoholic fatty liver disease: A systematic review and meta-analysis of randomized controlled trials.

Meta-analysisn = 358

Systematic review and meta-analysis of RCTs evaluating Nigella sativa for nonalcoholic fatty liver disease (NAFLD) treatment. NS supplementation improved levels of ALT, AST, FBS, HDL, hs-CRP, and the severity of NAFLD, but had no effect on TC, LDL, TG, insulin, and TNF-α.

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2021·Journal of food biochemistry·Rahele Sadat Montazeri, Somaye Fatahi, Mohammad Hassan Sohouli, et al

The effect of nigella sativa on biomarkers of inflammation and oxidative stress: A systematic review and meta-analysis of randomized controlled trials.

Meta-analysisInflammation Inflammation Inflammation Inflammation

This systematic review and meta-analysis evaluated the effects of Nigella Sativa seed and seed oil on biomarkers of inflammation and oxidative stress. The analysis of 10 RCTs showed that NS consumption significantly reduced serum hs-CRP, TNF-α, and MDA, and increased TAC and SOD levels, suggesting potential anti-inflammatory and antioxidant effects.

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2020·Pharmacological research·Elham Razmpoosh, Sara Safi, Nooshin Abdollahi, et al

The effect of Nigella sativa on the measures of liver and kidney parameters: A systematic review and meta-analysis of randomized-controlled trials.

Meta-analysisn = 1,295

This systematic review and meta-analysis evaluated the effect of Nigella sativa supplementation on liver and kidney parameters across 19 trials with 1295 participants. N.S supplements significantly reduced alkaline-phosphatase (ALP) and blood urea nitrogen (BUN) levels, with longer interventions reducing aspartate-aminotransferase (AST) measures. Higher doses increased BUN levels, indicating dose-dependent effects on liver and kidney parameters.

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2020·Complementary therapies in medicine·Mohammad Reza Mahmoodi, Mehdi Mohammadizadeh

Therapeutic potentials of Nigella sativa preparations and its constituents in the management of diabetes and its complications in experimental animals and patients with diabetes mellitus: A systematic review.

Systematic reviewInflammation Heart Health Immunity Nutrition

Systematic review evaluating the effects of Nigella sativa on diabetes management and its complications. N. sativa preparations show antidiabetic, hypoglycemic, anti-hyperlipidemic, antihypertensive, and anti-obesity effects, improving antioxidant defense, reducing inflammation, and enhancing endothelial, hepatic, kidney, heart, and immune functions.

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2020·Complementary therapies in medicine·Mohsen Mohit, Amirhosein Farrokhzad, Seyed Nooreddin Faraji, et al

Effect of Nigella sativa L. supplementation on inflammatory and oxidative stress indicators: A systematic review and meta-analysis of controlled clinical trials.

Meta-analysisInflammation Inflammation -Inflammation

Systematic review and meta-analysis of RCTs assessing Nigella sativa supplementation on inflammatory and oxidative stress markers. Nigella sativa significantly reduced CRP and MDA concentrations and increased TAC levels, but did not affect TNF-α.

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2020·Phytotherapy research : PTR·Jamal Hallajzadeh, Alireza Milajerdi, Moein Mobini, et al

Effects of Nigella sativa on glycemic control, lipid profiles, and biomarkers of inflammatory and oxidative stress: A systematic review and meta-analysis of randomized controlled clinical trials.

Meta-analysisInflammation -Nutrition

Systematic review and meta-analysis of 50 trials evaluating the effects of Nigella sativa on glycemic control, lipid profiles, and biomarkers of inflammatory and oxidative stress. Significant reductions were found in total cholesterol, triglycerides, LDL-cholesterol, VLDL-cholesterol, fasting glucose, and HbA1C levels following supplementation with N. sativa. Effects on CRP, TNF-α, TAC, and MDA levels were insignificant.

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2020·Journal of ethnopharmacology·Reza Mohebbati, Abbasali Abbasnezhad

Effects of Nigella sativa on endothelial dysfunction in diabetes mellitus: A review.

Systematic reviewHeart Health Inflammation

This systematic review summarizes the therapeutic effects of Nigella sativa on endothelial dysfunction in diabetes. It highlights the protective effects of Nigella sativa and Thymoquinone through mechanisms such as reducing inflammatory markers, improving hyperglycemia and hyperlipidemia, and inhibiting platelet aggregation.

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2018·Complementary therapies in medicine·Seyed Mohammad Mousavi, Ali Sheikhi, Hamed Kord Varkaneh, et al

Effect of Nigella sativa supplementation on obesity indices: A systematic review and meta-analysis of randomized controlled trials.

Meta-analysisn = 875Physique

This systematic review and meta-analysis examined the effects of Nigella sativa supplementation on obesity indices, including body weight, BMI, and waist circumference, in adults. The analysis included 13 RCTs with 875 subjects and found that Nigella sativa significantly reduced body weight and BMI compared to placebo, but had no significant effect on waist circumference.

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2016·Journal of hypertension·Amirhossein Sahebkar, Davide Soranna, Xiaoqiu Liu, et al

A systematic review and meta-analysis of randomized controlled trials investigating the effects of supplementation with Nigella sativa (black seed) on blood pressure.

Meta-analysisn = 860Heart Health

Systematic review and meta-analysis of 11 RCTs with 860 individuals investigating the effects of Nigella sativa on blood pressure. The analysis found that short-term treatment with N. sativa powder significantly reduced systolic and diastolic blood pressure levels. No publication bias was detected, but more evidence is needed to explore long-term effects.

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2025·Journal of ovarian research·Azamsadat Mahmoudian, Akram Ashouri, Fatemeh Mohammadzadeh, et al

Effect of Nigella sativa-L supplementation on glycemia in adolescent polycystic ovarian syndrome: secondary analysis of a randomized controlled trial study.

RCTn = 103Womens Health Nutrition

This secondary analysis of a randomized controlled trial evaluated the effects of Nigella sativa extract on glycemia in 116 adolescent girls with PCOS. The Nigella sativa group showed significant reductions in fasting plasma glucose and two-hour post-prandial glucose levels compared to the control group, suggesting potential benefits for managing glycemia in adolescent PCOS.

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2025·PloS one·Mahsa Maghalian, Afsaneh Alizadeh, Fatemeh Raphi, et al

The effect of Nigella Sativa emulgel on episiotomy wound healing and pain intensity in primiparous women: A triple-blind randomized controlled trial.

RCTn = 74Inflammation

A triple-blind RCT evaluated the efficacy of Nigella sativa emulgel on episiotomy wound healing and pain intensity in 74 primiparous women. The Nigella sativa group showed significantly better wound healing and lower pain scores compared to placebo, with high patient satisfaction and no adverse effects reported.

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2025·Paediatric drugs·Dalia El-Afify, Doaa El Amrousy

Cardioprotective Effect of Nigella sativa in Pediatric Patients with Type 1 Diabetes Mellitus: A Randomized Controlled Study.

RCTn = 60Heart Health

RCT of 60 children and adolescents with type 1 diabetes comparing Nigella sativa seed oil supplementation to insulin alone for 3 months. Nigella sativa improved lipid profile, reduced inflammatory markers, and enhanced cardiac function compared to control.

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2025·Narra J·Khairani Sukatendel, Reni H Hasibuan, Muhammad Fg Siregar, et al

Effect ofseed extract on estradiol, FSH levels, and vaginal maturity index in menopausal women: A randomized controlled trial.

RCTn = 50Womens Health Hormone Health

RCT conducted at Haji Adam Malik Hospital in Indonesia with 50 postmenopausal women, testing the effects of Nigella sativa seed extract on estradiol, FSH levels, and vaginal maturity index. The study found significant increases in estradiol levels and vaginal maturity index with no significant effect on FSH levels after eight weeks of treatment.

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2024·Journal of ovarian research·Azamsadat Mahmoudian, Akram Ashouri, Roghaieh Rahmani Bilandi, et al

The possible short-term of Nigella sativa - L in the management of adolescent polycystic ovarian syndrome: results of a randomized controlled trial.

RCTn = 103Womens Health

RCT of 114 adolescents with PCOS comparing Nigella sativa supplementation to medroxyprogesterone. Nigella sativa significantly improved hirsutism scores, ovarian volume, serum LH, testosterone levels, and reduced menstrual irregularities.

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2024·Prostaglandins & other lipid mediators·Shabnam Shirvani, Maryam Falahatzadeh, Elham Oveili, et al

The effect of Nigella sativa supplementation on glycemic status in adults: An updated systematic review and meta-analysis of randomized controlled trials.

Meta-analysisn = 30Nutrition

This systematic review and meta-analysis evaluated the effects of Nigella sativa consumption on glycemic index in adults, analyzing 30 RCTs. The results indicated that N. sativa supplementation significantly reduced fasting blood sugar (FBS) and HA1c levels, but had no significant effect on insulin or HOMA-IR.

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2023·Inflammopharmacology·Seyed-Ali Khodaie, Haniyeh Nikkhah, Nasim Namiranian, et al

Topical Nigella sativa L. product: a new candidate for the management of diabetic peripheral neuropathy.

RCTn = 120Inflammation

Double-blind clinical trial on 120 neuropathic patients comparing topical Nigella sativa ointment, placebo, and gabapentin. The Nigella sativa group showed significant improvements in neuropathy symptoms such as numbness, burning pain, and muscle cramps compared to the other groups.

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2023·International journal of fertility & sterility·Ali Hajb, Zeynab Salehpour, Roghayyeh Aghaei, et al

The Effect of Palm Pollen and Black Seed Pollen on Male Sex Hormones and Sperm Quality: A Single-Blind, Placebo-Controlled Clinical Trial Study.

RCTn = 62Sexual Health Hormone Health

Single-blind, placebo-controlled clinical trial of 62 infertile men testing palm pollen and black seed pollen extracts on sex hormones and sperm quality. The treatment group showed significant improvements in sperm count, motility, and hormone levels compared to placebo.

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2023·Phytotherapy research : PTR·Afsaneh Alizadeh, Sakineh Mohammah-Alizadeh-Charandabi, Laleh Khodaie, et al

Effect of Nigella sativa L. seed oil on urinary incontinence and quality of life in menopausal women: A triple-blind randomized controlled trial.

RCTn = 60Womens Health Sexual Health -

Triple-blind RCT of 60 menopausal women in Tabriz-Iran comparing Nigella sativa L. seed oil to placebo for 8 weeks. The treatment group showed significant improvement in mixed urinary incontinence and incontinence-related quality of life, but no significant differences in stress incontinence, urge incontinence, sexual function, or menopause-specific QoL.

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2023·F1000Research·Ayad Salem, Abdullah Bamosa, Mohammed Alam, et al

Effect ofon general health and immune system in young healthy volunteers; a randomized, placebo-controlled, double-blinded clinical trial.

RCTn = 52Immunity

This randomized, placebo-controlled, double-blinded clinical trial evaluated the effect of Nigella sativa on immunity-related parameters in 52 young healthy subjects. The study found that a 1 g dose of Nigella sativa significantly elevated total lymphocyte count, CD3+, and CD4+ counts, suggesting a promising immunopotentiation effect.

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2023·BMC complementary medicine and therapies·Hedieh Yousefnejad, Farzaneh Mohammadi, Mahvash Alizadeh-Naini, et al

Nigella sativa powder for helicobacter pylori infected patients: a randomized, double-blinded, placebo-controlled clinical trial.

RCTn = 51Nutrition

This double-blind, placebo-controlled clinical trial evaluated the effects of Nigella sativa powder plus conventional treatment on serum ghrelin levels and appetite in 51 H. pylori-infected patients. The treatment group showed significant improvement in appetite compared to the placebo group, although changes in serum ghrelin levels were not statistically significant.

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2022·Frontiers in Pharmacology·Sh. A. Said, Alsayyed Abdulbaset, Amal A El-Kholy, et al

The effect of Ni gella sativa and vitamin D3 supplementation on the clinical outcome in COVID-19 patients: A randomized controlled clinical trial

RCTn = 120Immunity

This randomized controlled trial investigated the effects of Nigella sativa and vitamin D3, both individually and in combination, on COVID-19 patients with mild to moderate symptoms. The combination of Nigella sativa and vitamin D3 significantly alleviated COVID-19 symptoms and improved viral clearance compared to other groups.

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2022·Complementary therapies in clinical practice·Hasan Fallah Huseini, Reza Mohtashami, Elaheh Sadeghzadeh, et al

Efficacy and safety of oral Nigella sativa oil for symptomatic treatment of knee osteoarthritis: A double-blind, randomized, placebo-controlled clinical trial.

RCTn = 116Joint Bone Health

Double-blind, randomized, placebo-controlled trial evaluating the efficacy and safety of oral Nigella sativa oil in 116 patients with knee osteoarthritis. NS oil significantly reduced VAS and WOMAC scores and decreased acetaminophen use compared to placebo, with no side effects reported.

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2022·Oral and maxillofacial surgery·Lokesh Kumar S, Zameera Naik, Arun Panwar, et al

Comparative evaluation of the efficacy of Nigella sativa (75% v/v) cream and clobetasol propionate (0.05% w/w) gel in oral lichen planus-a double-blinded randomized control trial.

RCTn = 60Skin Hair Health

Double-blinded RCT comparing Nigella sativa cream to clobetasol propionate gel in 60 patients with oral lichen planus. Both treatments significantly reduced burning sensation and lesion size, with Nigella sativa showing comparable efficacy to clobetasol without side effects.

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2022·Phytotherapy research : PTR·Omid Mohammad Tavakoli-Rouzbehani, Mohsen Abbasnezhad, Sorayya Kheirouri, et al

Efficacy of nigella sativa oil on endothelial function and atherogenic indices in patients with coronary artery diseases: A randomized, double-blind, placebo-control clinical trial.

RCTn = 60Heart Health Inflammation

RCT of 60 CAD patients comparing 2g daily Nigella sativa oil to sunflower oil placebo for 8 weeks. NS oil supplementation significantly reduced serum levels of sVCAM-1, sICAM-1, and MDA, while increasing total antioxidant capacity, suggesting potential benefits for endothelial function.

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2022·Phytotherapy research : PTR·Hadi Emamat, Seyyed Hossein Mousavi, Jalal Kargar Shouraki, et al

The effect of Nigella sativa oil on vascular dysfunction assessed by flow-mediated dilation and vascular-related biomarkers in subject with cardiovascular disease risk factors: A randomized controlled trial.

RCTn = 50Heart Health

RCT evaluating the effect of Nigella sativa oil on vascular function in 50 subjects with cardiovascular disease risk factors. The study found significant improvements in flow-mediated dilation and plasma nitric oxide levels in the Nigella sativa group compared to placebo, but no significant differences in ICAM-1 and VCAM-1 levels.

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2021·Complementary therapies in medicine·Abdulrahman E Koshak, Emad A Koshak, Abdullah F Mobeireek, et al

Nigella sativa for the treatment of COVID-19: An open-label randomized controlled clinical trial.

RCTn = 173Immunity

Open-label RCT evaluating Nigella sativa oil (NSO) in 173 patients with mild COVID-19 symptoms. The NSO group showed a higher recovery rate (62%) compared to the control group (36%), with a shorter mean duration to recovery.

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2021·Phytotherapy research : PTR·Azizeh Farshbaf-Khalili, Safar Farajnia, Samira Pourzeinali, et al

The effect of nanomicelle curcumin supplementation and Nigella sativa oil on the expression level of miRNA-21, miRNA-422a, and miRNA-503 gene in postmenopausal women with low bone mass density: A randomized, triple-blind, placebo-controlled clinical trial with factorial design.

RCTn = 120Joint Bone Health

This randomized, triple-blind, placebo-controlled clinical trial investigated the effect of nanomicelle curcumin and Nigella sativa oil on miRNA expression in 120 postmenopausal women with low bone mass density. The study found that Nigella sativa and its combination with curcumin significantly increased the expression level of miRNA-21, suggesting potential benefits for bone health.

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2021·Journal of diabetes and its complications·Tarek M Mostafa, Sahar K Hegazy, Sherin S Elnaidany, et al

Nigella sativa as a promising intervention for metabolic and inflammatory disorders in obese prediabetic subjects: A comparative study of Nigella sativa versus both lifestyle modification and metformin.

RCTn = 117Inflammation Nutrition

RCT comparing Nigella sativa, lifestyle modification, and metformin in 117 obese prediabetic subjects. Nigella sativa improved lipid panel, reduced TNF-α level, and Castelli risk index-I, showing similar effects to metformin on anthropometric and glycemic parameters, and improved SIRT1 gene expression.

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2021·Phytotherapy research : PTR·Omid Mohammad Tavakoli-Rouzbehani, Mohsen Abbasnezhad, Sorayya Kheirouri, et al

Effects of Nigella sativa oil supplementation on selected metabolic parameters and anthropometric indices in patients with coronary artery disease: A randomized, double-blind, placebo-controlled clinical trial.

RCTn = 60Heart Health Nutrition Physique

RCT of 60 CAD patients comparing 2g Nigella sativa oil to sunflower oil placebo for 8 weeks. NS significantly reduced weight, BMI, waist and hip circumference, blood pressure, and fasting blood glucose, indicating potential benefits on metabolic parameters.

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2021·Phytotherapy research : PTR·Parisa Shoaei-Hagh, Farzaneh Kamelan Kafi, Sara Najafi, et al

A randomized, double-blind, placebo-controlled, clinical trial to evaluate the benefits of Nigella sativa seeds oil in reducing cardiovascular risks in hypertensive patients.

RCTn = 55Heart Health Inflammation Nutrition

RCT evaluating Nigella sativa seeds oil in hypertensive patients. The intervention group showed significant reductions in systolic and diastolic blood pressure, total cholesterol, LDL, malondialdehyde, and fasting blood sugar, and increases in HDL and glutathione reductase, compared to placebo. No adverse events were reported.

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2021·Food Science & Nutrition·Saeid Hadi, Mahsa Miryan, Davood Soleimani, et al

The effect of food ration bar enriched with β‐alanine, L‐arginine, and Nigella sativa on performance and inflammation following intense military training: A double‐blind randomized clinical trial

RCTn = 54Physical Performance Inflammation

This double-blind, randomized clinical trial evaluated the effect of a food ration bar enriched with β-alanine, L-arginine, and Nigella sativa on athletic performance and inflammation in 54 new cadets undergoing intense military training. The intervention group showed significant improvements in anaerobic performance and reductions in inflammation markers compared to the control group.

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2020·Endocrine·Mahboobeh Shirazi, Fatemeh Khodakarami, Elham Feizabad, et al

The effects of nigella sativa on anthropometric and biochemical indices in postmenopausal women with metabolic syndrome.

RCTn = 140Heart Health Nutrition

This randomized, double-blinded, placebo-controlled trial studied the effects of 500 mg nigella sativa on anthropometric and biochemical indices in 140 postmenopausal women with metabolic syndrome. The nigella sativa group showed significant decreases in low-density lipoprotein, triglyceride, total cholesterol, and fasting blood sugar compared to placebo.

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2020·Phytotherapy research : PTR·Samaneh Soleymani, Arman Zargaran, Mohammad Hosein Farzaei, et al

The effect of a hydrogel made by Nigella sativa L. on acne vulgaris: A randomized double-blind clinical trial.

RCTn = 60Skin Hair Health

RCT of 60 patients with acne vulgaris comparing Nigella sativa hydrogel to placebo. The N. sativa group showed a 78% reduction in IGA score and significant reductions in comedones, papules, and pustules, with no adverse events.

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2019·Complementary therapies in medicine·Rahele Tavakoly, Arman Arab, Natalia Vallianou, et al

The effect of Nigella sativa L. supplementation on serum C-reactive protein: A systematic review and meta-analysis of randomized controlled trials.

Meta-analysisInflammation

Systematic review and meta-analysis of RCTs examining the effect of Nigella sativa supplementation on plasma C-reactive protein (CRP) concentrations. The meta-analysis showed a significant reduction in serum CRP, particularly in participants with BMI ≥ 30 kg/m.

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2019·BMC complementary and alternative medicine·Abid Nordin, Haziq Kamal, Muhammad Dain Yazid, et al

Effect of Nigella sativa and its bioactive compound on type 2 epithelial to mesenchymal transition: a systematic review.

Systematic reviewInflammation

Systematic review of 22 studies on Nigella sativa and its bioactive compound thymoquinone, focusing on their effects on type 2 epithelial to mesenchymal transition (EMT). The review found that Nigella sativa and thymoquinone promote wound healing, attenuate tissue inflammation, and prevent organ fibrosis by regulating the EMT process.

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2019·The American journal of emergency medicine·Tao He, Xiaohong Xu

The influence of Nigella sativa for asthma control: A meta-analysis.

Meta-analysis

Meta-analysis of four RCTs assessing Nigella sativa supplementation for asthma control. Nigella sativa is associated with increased ACT scores and FEV1, but shows no significant impact on PEF, IL-4, or IFN-γ. The study suggests potential benefits for asthma treatment.

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2019·Phytotherapy research : PTR·Gholamreza Askari, Mohammad Hossein Rouhani, Ehsan Ghaedi, et al

Effect of Nigella sativa (black seed) supplementation on glycemic control: A systematic review and meta-analysis of clinical trials.

Meta-analysisNutrition

Systematic review and meta-analysis of 17 randomized controlled trials assessing the antihyperglycemic effect of Nigella sativa on glycemic indices. The analysis found significant reductions in fasting plasma glucose, postprandial blood glucose, and hemoglobin A1c with Nigella sativa supplementation, with the oil form being more effective than the powder.

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2018·Journal of animal physiology and animal nutrition·P Kumar, A K Patra, G P Mandal, et al

Carcass characteristics, chemical and fatty acid composition and oxidative stability of meat from broiler chickens fed black cumin (Nigella sativa) seeds.

RCTn = 360Nutrition

The study investigated the effects of dietary supplementation of black cumin seeds on carcass characteristics, chemical and fatty acid composition, and antioxidant properties of broiler chicken meat. Results showed that black cumin seed supplementation improved slaughter body weight, beneficial fatty acid concentrations, and antioxidant properties of the meat.

PubMedRead on PubMed
2018·Journal of ethnopharmacology·Nazli Namazi, Bagher Larijani, Mohammad Hossein Ayati, et al

The effects of Nigella sativa L. on obesity: A systematic review and meta-analysis.

Meta-analysisPhysique

Systematic review and meta-analysis of 11 studies on the effects of Nigella sativa supplementation on obesity-related anthropometric indices. N. sativa supplementation significantly reduced body weight, BMI, and waist circumference compared to placebo. The study notes moderate effects and high heterogeneity for body weight, suggesting cautious interpretation.

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2017·Nutrients·Marco Cascella, Giuseppe Palma, Antonio Barbieri, et al

Role of Nigella sativa and Its Constituent Thymoquinone on Chemotherapy-Induced Nephrotoxicity: Evidences from Experimental Animal Studies.

Systematic review

Systematic review of experimental animal studies assessing the protective effects of Nigella sativa and its compound thymoquinone on chemotherapy-induced nephrotoxicity. The review included 12 studies and found that NS/TQ decreased lipid peroxidation and increased antioxidant enzyme activity in renal tissue, showing protective effects against nephrotoxicity.

PubMedRead on PubMed
2017·Complementary therapies in medicine·Reza Daryabeygi-Khotbehsara, Mahdieh Golzarand, Mohammad Payam Ghaffari, et al

Nigella sativa improves glucose homeostasis and serum lipids in type 2 diabetes: A systematic review and meta-analysis.

Meta-analysisNutrition Heart Health

Systematic review and meta-analysis of seven trials assessing Nigella sativa supplementation in type 2 diabetes. N. sativa significantly improved fasting blood sugar, HbA1c, total cholesterol, and LDL-cholesterol. Subgroup analysis showed significant reduction in triglycerides with N. sativa seed oil, while seed powder increased triglycerides.

PubMedRead on PubMed
2016·Pharmacological research·Amirhossein Sahebkar, Guglielmo Beccuti, Luis E Simental-Mendía, et al

Nigella sativa (black seed) effects on plasma lipid concentrations in humans: A systematic review and meta-analysis of randomized placebo-controlled trials.

Meta-analysisHeart Health

Systematic review and meta-analysis of 17 RCTs examining Nigella sativa's effects on plasma lipid concentrations. NS supplementation significantly reduced total cholesterol, LDL-C, and triglyceride levels, with no significant effect on HDL-C concentrations. NS seed oil showed greater effects on cholesterol and LDL-C, while NS seed powder increased HDL-C.

PubMedRead on PubMed
2015·Complementary therapies in medicine·Javad Heshmati, Nazli Namazi

Effects of black seed (Nigella sativa) on metabolic parameters in diabetes mellitus: a systematic review.

Systematic reviewNutrition

Systematic review of 19 articles (2 human trials, 14 animal models, and 3 in vivo/in vitro studies) on the effects of Nigella sativa on metabolic parameters in diabetes mellitus. The review suggests that N. sativa can modulate hyperglycemia and lipid profile dysfunction through various mechanisms, including antioxidant properties and effects on insulin secretion and glucose absorption.

PubMedRead on PubMed
2014·Anti-inflammatory & anti-allergy agents in medicinal chemistry·Amina Hamed Ahmad Alobaidi

Effect of Nigella sativa and Allium sativum coadminstered with simvastatin in dyslipidemia patients: a prospective, randomized, double-blind trial.

RCTn = 258Heart Health

A randomized, double-blind, placebo-controlled trial evaluated the effect of Nigella sativa (black seed) and Allium sativum (garlic) coadministered with simvastatin in dyslipidemia patients. The study found significant improvements in lipid profiles, including cholesterol, triglyceride, Non-HDL, and LDL levels, suggesting the combination was effective in correcting dyslipidemia.

PubMedRead on PubMed
2025·Journal of long-term effects of medical implants·Arunraaj Thanasekaran, Arvina Rajasekar

Therapeutic Potential of Thymoquinone in Conjunction with Mechanical Debridement for Peri-Implantitis: A Prospective Clinical Evaluation.

RCTn = 40Inflammation Joint Bone Health

This prospective clinical evaluation assessed the efficacy of locally applied thymoquinone gel combined with mechanical debridement in treating peri-implantitis. The study included 40 participants, with Group 2 receiving thymoquinone gel showing significantly better outcomes in clinical parameters compared to mechanical debridement alone.

PubMedRead on PubMed
2024·BMC complementary medicine and therapies·Elham Razmpoosh, Sara Safi, Mahta Mazaheri, et al

A crossover randomized controlled trial examining the effects of black seed (Nigella sativa) supplementation on IL-1β, IL-6 and leptin, and insulin parameters in overweight and obese women.

RCTn = 46Inflammation Hormone Health Nutrition

Crossover RCT of 46 overweight/obese women examining the effects of Nigella sativa supplementation on inflammation and insulin parameters. NS supplementation significantly reduced mRNA expressions and serum levels of IL-1β, IL-6, and leptin, with varying effect sizes. A significant decrease in serum insulin was also observed.

PubMedRead on PubMed
2024·European review for medical and pharmacological sciences·S Doğru, S Taysi, A Yücel

Effects of thymoquinone in the lungs of rats against radiation-induced oxidative stress.

RCTn = 40Inflammation

Prospective, placebo-controlled study on 40 Sprague-Dawley rats to test the radioprotective effectiveness of thymoquinone (TQ) against radiation-induced oxidative stress in lung tissue. TQ treatment resulted in lower oxidative stress parameters and higher anti-oxidative parameters, indicating its protective effects.

PubMedRead on PubMed
2024·Clinical and experimental dental research·Atessa Pakfetrat, Zahra Delavarian, Mahshid Malakooti, et al

Comparing the efficacy of a novel mucoadhesive patch containing Nigella sativa 10% with triamcinolone 0.1% in patients with erosive-atrophic oral lichen planus: A pilot study.

RCTn = 20Inflammation

Pilot RCT comparing a mucoadhesive patch containing Nigella sativa 10% extract to triamcinolone 0.1% in 20 patients with erosive-atrophic oral lichen planus. Both treatments reduced pain, burning, and lesion severity, with N. sativa showing superior results in some sessions. No significant complications were observed with N. sativa.

PubMedRead on PubMed
2022·Complementary therapies in clinical practice·Mohammad Rashidmayvan, Sarvenaz Vandyousefi, Meisam Barati, et al

The effect of nigella sativa supplementation on cardiometabolic outcomes in patients with non-alcoholic fatty liver: A randomized double-blind, placebo-controlled trial.

RCTn = 44

This randomized, double-blind, placebo-controlled trial assessed the effect of 1000 mg/day Nigella sativa oil supplementation on adiponectin, leptin, and blood pressure in 44 NAFLD patients over eight weeks. No statistically significant differences were found in these cardiometabolic measures between the Nigella sativa and placebo groups.

PubMedRead on PubMed
2021·Phytotherapy Research·Saeid Hadi, Reza Daryabeygi-Khotbehsara, P. Mirmiran, et al

Effect of Nigella sativa oil extract on cardiometabolic risk factors in type 2 diabetes: A randomized, double‐blind, placebo‐controlled clinical trial

RCTn = 43Heart Health Inflammation Nutrition

A randomized, double-blind, placebo-controlled clinical trial on 43 patients with type 2 diabetes assessed the effects of Nigella sativa oil extract on cardiometabolic risk factors. The intervention group received Nigella sativa oil, which significantly improved glycemic control, serum lipid profile, blood pressure, and body weight compared to placebo.

Semantic ScholarRead on Semantic Scholar
2020·Complementary therapies in medicine·Sara Safi, Elham Razmpoosh, Hossien Fallahzadeh, et al

The effect of Nigella sativa on appetite, anthropometric and body composition indices among overweight and obese women: A crossover, double-blind, placebo-controlled, randomized clinical trial.

RCTn = 45Physique Nutrition

Crossover, double-blind, placebo-controlled RCT evaluating the effect of Nigella sativa on anthropometric and body composition parameters in 45 overweight and obese women. Supplementation with 2000 mg of N. sativa oil led to improvements in body mass index, body weight, waist circumference, body fat mass, body fat percent, and visceral fat area, along with a decrease in appetite.

PubMedRead on PubMed
2020·Clinical nutrition ESPEN·Mahdieh Abbasalizad Farhangi, Siroos Tajmiri

The effects of powdered black cumin seeds on markers of oxidative stress, intracellular adhesion molecule (ICAM)-1 and vascular cell adhesion molecule (VCAM)-1 in patients with Hashimoto's thyroiditis.

RCTn = 40Inflammation Heart Health Nutrition

RCT of 40 patients with Hashimoto's thyroiditis comparing powdered Nigella sativa seeds to placebo for 8 weeks. N. sativa significantly increased serum TAC, SOD, reduced MDA, VCAM-1, BMI, LDL cholesterol, and triglycerides, while increasing HDL cholesterol, indicating improvements in oxidative stress, endothelial dysfunction, and serum lipids.

PubMedRead on PubMed
2020·European journal of nutrition·Elham Razmpoosh, Sara Safi, Azadeh Nadjarzadeh, et al

The effect of Nigella sativa supplementation on cardiovascular risk factors in obese and overweight women: a crossover, double-blind, placebo-controlled randomized clinical trial.

RCTn = 39Heart Health

Crossover, double-blind, placebo-controlled RCT assessing the effect of Nigella sativa oil supplements on cardiovascular risk factors in obese and overweight women. The study found that N.S increased HDL cholesterol, reduced LDL cholesterol, TC/HDL-C ratio, serum glutamic-oxaloacetic transaminase, and systolic blood pressure, indicating beneficial effects on cardiovascular health.

PubMedRead on PubMed
2019·Journal of ethnopharmacology·Ahmad Khonche, Hasan Fallah Huseini, Mahdi Gholamian, et al

Standardized Nigella sativa seed oil ameliorates hepatic steatosis, aminotransferase and lipid levels in non-alcoholic fatty liver disease: A randomized, double-blind and placebo-controlled clinical trial.

RCTn = 120Heart Health

RCT of 120 patients with non-alcoholic fatty liver disease comparing 2.5mL standardized Nigella sativa seed oil to placebo every 12 hours for 3 months. The oil group showed significant reductions in hepatic steatosis, ALT, AST, triglycerides, LDL-C, and HDL-C levels compared to placebo, with no adverse effects observed.

PubMedRead on PubMed
2019·Complementary therapies in medicine·Mohammadreza Rafati, Arash Ghasemi, Majid Saeedi, et al

Nigella sativa L. for prevention of acute radiation dermatitis in breast cancer: A randomized, double-blind, placebo-controlled, clinical trial.

RCTn = 62Skin Hair Health

RCT evaluating Nigella sativa extract for preventing acute radiation dermatitis in 62 breast cancer patients undergoing radiotherapy. N. sativa gel significantly reduced the frequency of ARD, delayed the onset of moist desquamation, and reduced pain compared to placebo.

PubMedRead on PubMed
2019·Phytotherapy research : PTR·Mohammad Reza Ardakani Movaghati, Mahdi Yousefi, Seyed Ahmad Saghebi, et al

Efficacy of black seed (Nigella sativa L.) on kidney stone dissolution: A randomized, double-blind, placebo-controlled, clinical trial.

RCTn = 60

RCT of 60 patients with renal stones comparing black seed (Nigella sativa L.) capsules to placebo for 10 weeks. The black seed group showed significant positive effects on the disappearance or reduction of kidney stone size compared to placebo.

PubMedRead on PubMed
2019·Phytotherapy research : PTR·Mina Darand, Zahra Darabi, Zahra Yari, et al

The effects of black seed supplementation on cardiovascular risk factors in patients with nonalcoholic fatty liver disease: A randomized, double-blind, placebo-controlled clinical trial.

RCTn = 50Heart Health Inflammation

This randomized, double-blind, placebo-controlled clinical trial evaluated the effects of Nigella sativa supplementation on cardiovascular risk factors in 50 patients with nonalcoholic fatty liver disease. N. sativa supplementation led to significant reductions in serum glucose, serum insulin, insulin resistance, and hepatic steatosis compared to placebo, but no significant changes in lipid profile.

PubMedRead on PubMed
2019·Complementary therapies in medicine·Mina Darand, Zahra Darabi, Zahra Yari, et al

Nigella sativa and inflammatory biomarkers in patients with non-alcoholic fatty liver disease: Results from a randomized, double-blind, placebo-controlled, clinical trial.

RCTn = 50Inflammation

This randomized, double-blind, placebo-controlled clinical trial assessed the effects of Nigella sativa on inflammatory biomarkers in 50 NAFLD patients. Nigella sativa consumption significantly reduced serum levels of TNF-α, hs-CRP, and NF-κB, and decreased hepatic steatosis percentage compared to placebo.

PubMedRead on PubMed
2019·Endocrine·Hebatallah Ahmed Mohamed Moustafa, Lamia Mohamed El Wakeel, Mohamed Reda Halawa, et al

Effect of Nigella Sativa oil versus metformin on glycemic control and biochemical parameters of newly diagnosed type 2 diabetes mellitus patients.

RCTNutrition Inflammation

RCT comparing Nigella sativa oil to metformin in newly diagnosed type 2 diabetes mellitus patients over 3 months. NS was inferior to metformin in lowering FBG, 2h pp, and A1C but comparable in reducing weight, WC, and BMI. NS showed no side effects and was comparable to metformin in effects on fasting insulin, %S, IR, ALT, TC, LDL, HDL, TG, and TAC.

PubMedRead on PubMed
2018·Iranian Red Crescent Medical Journal·S. M. Hosseini, Gholamreza Ghayour Razmgah, M. Nematy, et al

Efficacy of Black Seed ( Nigella sativa ) and Lemon Balm ( Melissa officinalis ) on Non-Alcoholic Fatty Liver Disease: A Randomized Controlled Clinical Trial

RCTn = 50

RCT evaluating Nigella sativa and Melissa officinalis in 50 patients with Non-Alcoholic Fatty Liver Disease. The intervention group showed significant reductions in liver enzymes, body mass index, and grade of fatty liver compared to baseline and control.

Semantic ScholarRead on Semantic Scholar
2017·IOP Conference Series: Materials Science and Engineering·P. N. R. Rachman, Akrom, E. Darmawan

The efficacy of black cumin seed (Nigella sativa) oil and hypoglycemic drug combination to reduce HbA1c level in patients with metabolic syndrome risk

RCTn = 99Nutrition

Randomized single-blind controlled trial with 99 outpatients at risk of metabolic syndrome. Patients received black cumin seed oil at doses of 1.5 mL/day and 3 mL/day for 20 days. The study found that black cumin seed oil combined with hypoglycemic drugs significantly reduced HbA1c levels.

Semantic ScholarRead on Semantic Scholar
2017·Phytotherapy research : PTR·Abdulrahman Koshak, Li Wei, Emad Koshak, et al

Nigella sativa Supplementation Improves Asthma Control and Biomarkers: A Randomized, Double-Blind, Placebo-Controlled Trial.

RCTn = 80Inflammation

RCT of 80 asthmatics comparing 500mg Nigella sativa oil capsules twice daily to placebo for 4 weeks. NSO group showed significant improvement in Asthma Control Test score and reduction in blood eosinophils, with a trend towards improved pulmonary function.

PubMedRead on PubMed
2017·Acta medica Indonesiana·Aulia Rizka, Siti Setiati, Aida Lydia, et al

Effect of Nigella sativa Seed Extract for Hypertension in Elderly: a Double-blind, Randomized Controlled Trial.

RCTn = 76Heart Health -

Double-blind, randomized controlled trial of 76 elderly patients with hypertension comparing 300 mg Nigella sativa seed extract twice daily to placebo for 28 days. The study found no significant effect of Nigella sativa on systolic or diastolic blood pressure compared to placebo.

PubMedRead on PubMed
2017·Journal of Ayub Medical College, Abbottabad : JAMC·Mazhar Hussain, Ali Gul Tunio, Lubna Akhtar, et al

Effects of nigella sativa on various parameters in Patients of non-alcoholic fatty liver disease.

RCTn = 70

RCT evaluating the effects of Nigella sativa on NAFLD patients. The study found significant reductions in body weight, BMI, liver enzymes, and improved ultrasound findings of fatty liver after 12 weeks of treatment with Nigella sativa compared to placebo.

PubMedRead on PubMed
2015·Planta medica·Hasan Fallah Huseini, Saeed Kianbakht, Mohammad Hossein Mirshamsi, et al

Effectiveness of Topical Nigella sativa Seed Oil in the Treatment of Cyclic Mastalgia: A Randomized, Triple-Blind, Active, and Placebo-Controlled Clinical Trial.

RCTn = 156Womens Health

RCT comparing the effectiveness of topical Nigella sativa seed oil to topical diclofenac and placebo in 156 women with cyclic mastalgia. Nigella sativa seed oil significantly reduced pain scores compared to placebo and was as effective as diclofenac, with no adverse effects observed.

PubMedRead on PubMed
2015·PloS one·Huda Kaatabi, Abdullah Omar Bamosa, Ahmed Badar, et al

Nigella sativa improves glycemic control and ameliorates oxidative stress in patients with type 2 diabetes mellitus: placebo controlled participant blinded clinical trial.

RCTn = 114Nutrition Inflammation

RCT of 114 type 2 diabetic patients on oral hypoglycemic drugs, comparing Nigella sativa supplementation to placebo over one year. Nigella sativa improved fasting blood glucose, HbA1c, and oxidative stress markers, enhancing glucose homeostasis and antioxidant defense.

PubMedRead on PubMed
2015·Journal of ethnopharmacology·Reza Mohtashami, Hasan Fallah Huseini, Mojtaba Heydari, et al

Efficacy and safety of honey based formulation of Nigella sativa seed oil in functional dyspepsia: A double blind randomized controlled clinical trial.

RCTn = 70Gut Health

Double-blind randomized controlled trial of 70 patients with functional dyspepsia receiving a honey-based formulation of Nigella sativa oil or placebo for 8 weeks. The N. sativa group showed significant improvement in dyspepsia severity and reduced H. pylori infection rates compared to placebo.

PubMedRead on PubMed
2014·Phytomedicine : international journal of phytotherapy and phytopharmacology·M Kolahdooz, S Nasri, S Zadeh Modarres, et al

Effects of Nigella sativa L. seed oil on abnormal semen quality in infertile men: a randomized, double-blind, placebo-controlled clinical trial.

RCTn = 68Sexual Health

RCT on Iranian infertile men with abnormal semen quality, comparing 2.5ml Nigella sativa oil to placebo twice daily for 2 months. The Nigella sativa oil group showed significant improvements in sperm count, motility, morphology, semen volume, pH, and round cells compared to placebo.

PubMedRead on PubMed
2013·Journal of animal physiology and animal nutrition·J Boka, A H Mahdavi, A H Samie, et al

Effect of different levels of black cumin (Nigella sativa L.) on performance, intestinal Escherichia coli colonization and jejunal morphology in laying hens.

RCTn = 100

This study investigated the effects of different levels of black cumin seeds on performance, intestinal E. coli count, and jejunal morphology in 100 laying hens. Black cumin supplementation decreased ileal E. coli enumeration, improved serum lipid profile, and enhanced eggshell quality. The best intestinal health indices and performance were observed with at least 2% black cumin seeds.

PubMedRead on PubMed
2013·Phytotherapy research : PTR·H Fallah Huseini, M Amini, R Mohtashami, et al

Blood pressure lowering effect of Nigella sativa L. seed oil in healthy volunteers: a randomized, double-blind, placebo-controlled clinical trial.

RCTn = 70Heart Health

Double-blind, randomized clinical trial of 70 healthy volunteers assessing the effect of Nigella sativa oil on blood pressure. The study found that oral administration of 5 mL N. sativa oil daily for 8 weeks significantly lowered systolic and diastolic blood pressures without adverse effects.

PubMedRead on PubMed
2013·The Journal of parasitology·A Nasir, M Avais, M S Khan, et al

Treating Cryptosporidium parvum infection in calves.

RCT

The study evaluated the efficacy of azithromycin, co-trimoxazole, and Nigella sativa (kalvangi) against Cryptosporidium parvum infection in calves. Azithromycin showed the highest efficacy, while co-trimoxazole and kalvangi were found to be ineffective under the treatment regimens used.

PubMedRead on PubMed
2012·Medical archives (Sarajevo, Bosnia and Herzegovina)·Ali Mohammad Sabzghabaee, Mehrnoush Dianatkhah, Nizal Sarrafzadegan, et al

Clinical evaluation of Nigella sativa seeds for the treatment of hyperlipidemia: a randomized, placebo controlled clinical trial.

RCTn = 88Heart Health

RCT conducted in Isfahan, Iran, with 88 subjects evaluating the efficacy of Nigella sativa seeds on hyperlipidemia. Significant decreases were observed in total cholesterol, LDL, and triglycerides, with no effect on fasting blood sugar and HDL.

PubMedRead on PubMed
2012·Journal of the European Academy of Dermatology and Venereology : JEADV·M Yousefi, B Barikbin, M Kamalinejad, et al

Comparison of therapeutic effect of topical Nigella with Betamethasone and Eucerin in hand eczema.

RCTn = 60Skin Hair Health

RCT comparing the effects of Nigella sativa, Betamethasone, and Eucerin on hand eczema severity and life quality in 60 patients. Nigella and Betamethasone showed significantly more rapid improvement in hand eczema and life quality compared to Eucerin, with no significant difference between Nigella and Betamethasone.

PubMedRead on PubMed
2010·Indian journal of physiology and pharmacology·Abdullah O Bamosa, Huda Kaatabi, Fatma M Lebdaa, et al

Effect of Nigella sativa seeds on the glycemic control of patients with type 2 diabetes mellitus.

RCTn = 94Nutrition

RCT of 94 patients with type 2 diabetes mellitus testing Nigella sativa as an adjuvant therapy. Nigella sativa at 2 gm/day significantly reduced fasting blood glucose, 2-hour postprandial glucose, and HbA1c without affecting body weight. Insulin resistance decreased and beta-cell function improved. No adverse effects on renal or hepatic functions were observed.

PubMedRead on PubMed
2010·American journal of otolaryngology·Soheila Nikakhlagh, Fakher Rahim, Faezeh Hossein Nejad Aryani, et al

Herbal treatment of allergic rhinitis: the use of Nigella sativa.

RCTn = 66Inflammation

This clinical trial investigated the anti-inflammatory effects of Nigella sativa on 66 patients with allergic rhinitis. The study found that N. sativa reduced nasal mucosal congestion, nasal itching, runny nose, sneezing attacks, turbinate hypertrophy, and mucosal pallor during the first 2 weeks.

PubMedRead on PubMed
2010·Acta medica Indonesiana·E A Datau, Wardhana, Eko E Surachmanto, et al

Efficacy of Nigella sativa on serum free testosterone and metabolic disturbances in central obese male.

RCTMens Health Physique Heart Health Inflammation -

Double-blind, placebo-controlled RCT studying the effects of Nigella sativa on central obese men. Significant reductions in body weight and waist circumference were observed, with no significant changes in serum free testosterone, blood pressure, or other metabolic markers. Nigella sativa may inhibit the decrease of serum free testosterone. No side effects were detected.

PubMedRead on PubMed
2009·Journal of alternative and complementary medicine (New York, N.Y.)·Waris Qidwai, Hasan Bin Hamza, Riaz Qureshi, et al

Effectiveness, safety, and tolerability of powdered Nigella sativa (kalonji) seed in capsules on serum lipid levels, blood sugar, blood pressure, and body weight in adults: results of a randomized, double-blind controlled trial.

RCTn = 123Heart Health Nutrition Physique

RCT conducted at Aga Khan University Hospital to assess the effectiveness, safety, and tolerability of powdered Nigella sativa (Kalonji) seed in capsules on serum lipid levels, blood sugar, blood pressure, and body weight in adults. The study found a favorable impact on almost all variables, but results were not statistically significant due to small sample size.

PubMedRead on PubMed
2008·International journal of clinical pharmacology and therapeutics·M Dirjomuljono, I Kristyono, R R Tjandrawinata, et al

Symptomatic treatment of acute tonsillo-pharyngitis patients with a combination of Nigella sativa and Phyllanthus niruri extract.

RCTn = 186Immunity Inflammation

RCT of 186 patients with acute tonsillopharyngitis comparing NSPN extract (Nigella sativa and Phyllanthus niruri) to placebo over 7 days. NSPN group showed significant alleviation of sore throat and required less analgesic therapy. NSPN extract was safe and well tolerated.

PubMedRead on PubMed
2008·Fundamental & clinical pharmacology·Farshad Roghani Dehkordi, Amir Farhad Kamkhah

Antihypertensive effect of Nigella sativa seed extract in patients with mild hypertension.

RCTHeart Health

RCT evaluating the antihypertensive effect of Nigella sativa seed extract in patients with mild hypertension. The study found significant reductions in both systolic and diastolic blood pressure in the treatment groups compared to placebo, with effects being dose-dependent. Additionally, there was a significant decline in total and LDL-cholesterol levels.

PubMedRead on PubMed
2003·Phytotherapy research : PTR·Ulrich Kalus, Axel Pruss, Jaromir Bystron, et al

Effect of Nigella sativa (black seed) on subjective feeling in patients with allergic diseases.

RCTn = 152Inflammation

Four studies on the clinical efficacy of Nigella sativa in 152 patients with allergic diseases showed that treatment with black seed oil decreased the subjective severity of symptoms. There was a slight decrease in plasma triglycerides and a discrete increase in HDL cholesterol, while lymphocyte subpopulations, endogenous cortisol levels, and ACTH release remained unchanged.

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2023·Antioxidants·Raiyan Sakib, F. Caruso, S. Aktar, et al

Antioxidant Properties of Thymoquinone, Thymohydroquinone and Black Cumin (Nigella sativa L.) Seed Oil: Scavenging of Superoxide Radical Studied Using Cyclic Voltammetry, DFT and Single Crystal X-ray Diffraction

Study

The study investigates the antioxidant activities of black seed oil, thymoquinone (TQ), and thymohydroquinone (THQ) using electrochemical methods. TQ and black seed oil showed good superoxide scavenging ability, while THQ did not. The study also explores the chemical mechanism of TQ's antioxidant activity using DFT and crystal structure analysis.

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2021·Bulletin of the National Research Centre·C. Duru, I. Duru, A. Adegboyega

In silico identification of compounds from Nigella sativa seed oil as potential inhibitors of SARS-CoV-2 targets

Study

In silico study identifying compounds from Nigella sativa seed oil as potential inhibitors of SARS-CoV-2 targets. Caryophyllene oxide, α-bergamotene, and β-bisabolene showed promising binding affinities to various SARS-CoV-2 molecular targets, suggesting potential antiviral properties.

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2021·Pharmaciana·Rodhia Ulfa, Dwi Utami, Iis Wahyuningsih

Development of black cumin seed oil (Nigella sativa L.) SNEDDS into solid-SNEDDS and its characterization

Study

The study developed a solid-SNEDDS formulation of black cumin seed oil (Nigella sativa L.) to improve its absorption and overcome the limitations of liquid-SNEDDS. The solid-SNEDDS was prepared using aerosil and crospovidone as adsorbents, with aerosil showing better performance under stress conditions.

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