Research
Nicotinamide Riboside
211 peer-reviewed studies curated from PubMed and Semantic Scholar.
Studies
Sorted by quality and recency
The Effect of Nicotinamide Mononucleotide and Riboside on Skeletal Muscle Mass and Function: A Systematic Review and Meta-Analysis.
Systematic review and meta-analysis of RCTs assessing the effects of nicotinamide mononucleotide (NMN) and nicotinamide riboside (NR) on skeletal muscle mass and function in older adults. NMN showed no significant effects on skeletal muscle index, handgrip strength, gait speed, or other measures of sarcopenia. NR was associated with a longer 6-minute walking distance in individuals with peripheral artery disease but showed no overall benefit for muscle mass and function.
Supplementation with NAD+ Precursors for Treating Alzheimer's Disease: A Metabolic Approach.
Systematic review of NAD+ precursors, including NMN and NR, for treating Alzheimer's disease. Preclinical studies show potential benefits of NMN and NR, with human trials of NR showing improvements in biomarkers and cognitive measures.
Gene expression profiles in sporadic ALS fibroblasts define disease subtypes and the metabolic effects of the investigational drug EH301.
The study investigates the transcriptome and metabolome of sporadic ALS fibroblasts to understand the impact of the investigational drug EH301, which includes nicotinamide riboside and pterostilbene. EH301 modifies gene expression differently in ALS subtypes and controls, showing protective effects against metabolic stress linked to anti-inflammatory and antioxidant pathways.
NAD+ therapy in age-related degenerative disorders: A benefit/risk analysis.
Systematic review of 147 articles evaluating the health and longevity benefits of increasing NAD+ levels. Most studies indicated favorable outcomes on age-related disorders, but potential risks include toxic metabolite accumulation and tumorigenesis. Long-term human trials are limited.
The differential impact of three different NAD+ boosters on circulatory NAD and microbial metabolism in humans
Randomized, open-label, placebo-controlled study in 65 healthy participants comparing the effects of NR, NMN, and Nam on circulatory NAD+ levels and gut microbial metabolism. NR and NMN increased circulatory NAD+ concentrations, while Nam acutely affected the NAD+ metabolome. NR and NMN also enhanced microbial growth and metabolism, suggesting a dual effect on systemic NAD+ levels and gut health.
Evaluation of Nicotinamide Riboside in Prevention of Small Nerve Fiber Axon Degeneration and Promotion of Nerve Regeneration.
Phase 2 placebo-controlled, double-blinded RCT investigating oral nicotinamide riboside (NR) supplementation for preventing small nerve fiber axon degeneration and promoting nerve regeneration. NR did not prevent degeneration or significantly improve nerve regeneration, though a small increase in epidermal sensory nerve fibers was observed at 60 days.
Nicotinamide riboside combined with exercise to treat hypertension in middle-aged and older adults: a pilot randomized clinical trial.
This pilot RCT tested the efficacy of combining aerobic exercise with nicotinamide riboside (NR) to enhance blood pressure control in 54 sedentary adults aged 55 and older with hypertension. The study found that NR combined with exercise did not significantly reduce systolic blood pressure compared to exercise alone, but showed trends toward greater nighttime blood pressure reduction in participants not on antihypertensive medication.
Cognitive and Alzheimer's disease biomarker effects of oral nicotinamide riboside (NR) supplementation in older adults with subjective cognitive decline and mild cognitive impairment
A crossover, double-blind, randomized placebo-controlled trial tested the safety and efficacy of 8 weeks' supplementation with nicotinamide riboside (1 g/day) on cognition and plasma Alzheimer's disease biomarkers in older adults with subjective cognitive decline and mild cognitive impairment.
Nicotinamide Riboside Supplementation Benefits in Patients With Werner Syndrome: A Double-Blind Randomized Crossover Placebo-Controlled Trial.
Double-blind, randomized, crossover, placebo-controlled trial in patients with Werner syndrome to evaluate nicotinamide riboside (NR) supplementation. NR improved arterial stiffness, decreased skin ulcer area, and showed a trend in reducing heel pad thinning. Metabolomic analysis indicated a decrease in blood creatinine, suggesting benefits for preventing atherosclerosis, skin ulcers, and kidney dysfunction.
Nicotinamide riboside for peripheral artery disease: the NICE randomized clinical trial.
This randomized double-blind clinical trial assessed the effects of nicotinamide riboside (NR), with and without resveratrol, on 6-min walk distance in 90 people with peripheral artery disease (PAD) over 6 months. NR significantly improved 6-min walk distance compared to placebo, with NR alone showing a greater improvement than NR combined with resveratrol.
Randomized, placebo-controlled, pilot clinical study evaluating acute Niagen+ IV and NAD+ IV in healthy adults
This randomized, placebo-controlled pilot study evaluated the safety, tolerability, and efficacy of intravenous nicotinamide riboside (NR IV) in boosting blood NAD+ levels in healthy adults. NR IV was found to be safe, with fewer adverse experiences compared to NAD+ IV, and it significantly increased NAD+ concentrations more than NAD+ IV and oral NR at the 3-hour timepoint.
Nicotinamide riboside as a neuroprotective therapy for glaucoma: study protocol for a randomized, double-blind, placebo-control trial
This is a study protocol for a randomized, double-blind, placebo-controlled trial investigating the neuroprotective effects of nicotinamide riboside (NR) in patients with primary open-angle glaucoma. The trial will include 125 patients, randomized to receive 300 mg NR or placebo for 24 months, with primary and secondary outcomes focused on optic nerve degeneration and visual field progression.
Nicotinamide riboside and pterostilbene reduces markers of hepatic inflammation in NAFLD: A double-blind, placebo-controlled clinical trial.
A 6-month double-blind, placebo-controlled clinical trial assessed the efficacy of NRPT (nicotinamide riboside and pterostilbene) supplementation in 111 adults with NAFLD. While no significant change was seen in hepatic fat fraction, a decrease in liver enzymes ALT and GGT and toxic lipid ceramide 14:0 was observed in the NRPT 1× group, indicating potential benefits in lowering markers of hepatic inflammation.
Nicotinamide Riboside Supplementation for Treating Elevated Systolic Blood Pressure and Arterial Stiffness in Midlife and Older Adults
This randomized, double-blind, placebo-controlled trial investigates the efficacy of nicotinamide riboside supplementation for 3 months in reducing systolic blood pressure and arterial stiffness in midlife and older adults with above-normal SBP. The study aims to establish the safety and efficacy of nicotinamide riboside in improving cardiovascular health.
Exercise training and NR supplementation to improve muscle mass and fitness in adolescent and young adult hematopoietic cell transplant survivors: a randomized controlled trial {1}
This randomized controlled trial tests the effects of 16 weeks of in-home aerobic and resistance exercise combined with nicotinamide riboside (NR) supplementation in adolescent and young adult hematopoietic cell transplant survivors. The primary outcome is muscle strength, with secondary outcomes including cardiovascular fitness, muscle mass, and mitochondrial function. The study aims to determine if exercise with NR produces larger improvements than exercise alone.
Exercise training and NR supplementation to improve muscle mass and fitness in adolescent and young adult hematopoietic cell transplant survivors: a randomized controlled trial {1}.
RCT testing 16 weeks of in-home aerobic and resistance exercise combined with nicotinamide riboside (NR) supplementation in adolescent and young adult hematopoietic cell transplant survivors. The study aims to improve muscle strength, cardiovascular fitness, muscle mass, and mitochondrial function. Eighty participants will be randomized to exercise + NR, exercise alone, NR alone, or control.
Nicotinamide Riboside Enhances In Vitro Beta-adrenergic Brown Adipose Tissue Activity in Humans.
The study investigated the effects of nicotinamide riboside (NR) on brown adipose tissue (BAT) activity. In vitro, NR enhanced norepinephrine-stimulated mitochondrial uncoupling in human BAT adipocytes. However, in a randomized, double-blinded, placebo-controlled, crossover study, NR supplementation did not alter BAT activity or cold-induced thermogenesis in human volunteers.
Nicotinamide Riboside as a Neuroprotective Therapy for Glaucoma Study Protocol for a Randomized, Double-blind, Placebo-control Trial
Study protocol for a randomized, double-blind, placebo-controlled trial investigating nicotinamide riboside as a neuroprotective therapy for glaucoma.
A Pilot Trial of Nicotinamide Riboside and Coenzyme Q10 on Inflammation and Oxidative Stress in CKD
Pilot randomized, double-blind, placebo-controlled crossover trial of nicotinamide riboside and coenzyme Q10 supplementation in 25 patients with moderate-to-severe CKD. Both supplements improved markers of oxidative stress, inflammation, and cell bioenergetics. Nicotinamide riboside altered gene expression related to metabolism and immune response, while coenzyme Q10 affected lipid metabolism and reduced inflammatory cytokines.
Qualia NAD+(R) Increases NAD+ In Human Participants: A Randomized, Double-Blind, Placebo-Controlled Study
This randomized, double-blind, placebo-controlled study examined the effects of Qualia NAD+ on whole blood NAD+ levels and quality of life measures in 25 healthy adults aged 40-65. Qualia NAD+ significantly increased NAD+ levels by 74% compared to a 4% increase in the placebo group. Improvements in overall and sexual quality of life were observed for males only.
Effect of nicotinamide riboside on airway inflammation in COPD: a randomized, placebo-controlled trial.
This randomized, placebo-controlled trial studied the effects of nicotinamide riboside (NR) on airway inflammation in 40 patients with stable COPD over 6 weeks, with a 12-week follow-up. NR treatment significantly reduced sputum IL-8 levels and increased NAD levels in whole blood, suggesting a positive effect on inflammation.
Randomized Crossover Clinical Trial of Nicotinamide Riboside and Coenzyme Q10 on Metabolic Health and Mitochondrial Bioenergetics in CKD
Randomized, double-blind, placebo-controlled crossover trial comparing 1200 mg/day of CoQ10 or 1000 mg/day of nicotinamide riboside (NR) supplementation to placebo in 25 people with moderate-to-severe CKD. NR increased plasma IL-2 and improved bioenergetic health index and spare respiratory capacity in monocytes. CoQ10 decreased IL-13 and CRP levels. Both supplements reduced oxidative stress markers.
Feasibility of telehealth exercise and nicotinamide riboside supplementation in survivors of childhood cancer at risk for diabetes: A pilot randomized controlled trial.
This pilot RCT assessed the feasibility of a telehealth exercise program combined with nicotinamide riboside supplementation in childhood cancer survivors with prediabetes. The study found high enrollment and compliance rates, but no significant differences in secondary endpoints between study arms. Myostatin decrease was linked to exercise compliance, suggesting potential benefits for muscle health.
Effect of the Mediterranean diet supplemented with nicotinamide riboside and pterostilbene and/or coconut oil on anthropometric variables in amyotrophic lateral sclerosis. A pilot study
Pilot study assessing the impact of a Mediterranean Diet supplemented with nicotinamide riboside, pterostilbene, and/or coconut oil on anthropometric variables in ALS patients. The study found that supplementation with antioxidants or coconut oil significantly increased muscle mass percentage and decreased fat mass percentage and skinfolds compared to the control group.
A randomized placebo-controlled trial of nicotinamide riboside in older adults with mild cognitive impairment.
A randomized placebo-controlled trial assessed the safety and effects of nicotinamide riboside (NR) in 20 older adults with mild cognitive impairment. NR significantly increased blood NAD+ levels but did not alter cognition. Cerebral blood flow was reduced in the default mode network, but this was not statistically significant after correction for multiple comparisons.
NR-SAFE: a randomized, double-blind safety trial of high dose nicotinamide riboside in Parkinson's disease.
A phase I randomized, double-blind, placebo-controlled trial evaluated the safety of high-dose nicotinamide riboside (NR) in 20 individuals with Parkinson's disease. NR therapy was well tolerated with no moderate or severe adverse events and showed clinical improvement in MDS-UPDRS scores, although this was influenced by the timing of levodopa dosing. NR significantly increased blood NAD levels.
A randomized placebo-controlled trial of nicotinamide riboside and pterostilbene supplementation in experimental muscle injury in elderly individuals.
RCT of 32 elderly individuals assessing the effects of nicotinamide riboside and pterostilbene supplementation on muscle regeneration after injury. The study found no effect of supplementation on muscle stem cell recruitment or muscle recovery measures.
The NADPARK study: A randomized phase I trial of nicotinamide riboside supplementation in Parkinson's disease.
A double-blinded phase I clinical trial of nicotinamide riboside (NR) supplementation in 30 newly diagnosed, treatment-naive Parkinson's disease patients. NR was well tolerated, increased cerebral NAD levels, altered cerebral metabolism, and was associated with mild clinical improvement. NR also decreased inflammatory cytokines and upregulated mitochondrial, lysosomal, and proteasomal functions.
Oral nicotinamide riboside raises NAD+ and lowers biomarkers of neurodegenerative pathology in plasma extracellular vesicles enriched for neuronal origin.
RCT of oral nicotinamide riboside (NR) supplementation in 22 healthy older adults. NR increased NAD levels in neuronal origin extracellular vesicles and decreased levels of biomarkers related to neurodegenerative pathology, such as Aβ42, pJNK, and pERK1/2.
A randomized placebo-controlled clinical trial of Nicotinamide Riboside and Pterostilbene supplementation in experimental muscle injury in elderly subjects
A randomized, placebo-controlled trial investigated the effects of Nicotinamide Riboside and Pterostilbene supplementation on muscle regeneration in elderly subjects after induced muscle injury. The study found no significant effect of the supplementation on muscle stem cell recruitment or other measures of muscle recovery.
Combined Metabolic Activators Accelerates Recovery in Mild-to-Moderate COVID-19.
Placebo-controlled, open-label phase 2 and double-blinded phase 3 clinical trials investigated the effect of combined metabolic activators (CMAs) on COVID-19 recovery. CMAs, including nicotinamide riboside, l-serine, N-acetyl-l-cysteine, and l-carnitine tartrate, significantly shortened recovery time and improved plasma levels of proteins and metabolites associated with inflammation and antioxidant metabolism.
Nicotinamide riboside with pterostilbene (NRPT) increases NAD+ in patients with acute kidney injury (AKI): a randomized, double-blind, placebo-controlled, stepwise safety study of escalating doses of NRPT in patients with AKI
Randomized, double-blind, placebo-controlled study of escalating doses of nicotinamide riboside and pterostilbene (NRPT) in 24 hospitalized patients with acute kidney injury (AKI). NRPT increased whole blood NAD+ levels by 37% at 48 hours compared to baseline, with a significant increase in Step 2. NRPT was safe and well tolerated, with minor gastrointestinal side effects reported.
Nicotinamide riboside supplementation alters body composition and skeletal muscle acetylcarnitine concentrations in healthy obese humans.
A randomized, double-blinded, placebo-controlled, crossover intervention study in 13 healthy overweight or obese men and women investigated the effects of 6 weeks of nicotinamide riboside (NR) supplementation. NR increased body fat-free mass and sleeping metabolic rate, and elevated skeletal muscle NAD+ metabolites and acetylcarnitine concentrations. No effects were found on insulin sensitivity, mitochondrial function, or other metabolic health parameters.
Boosting NAD level suppresses inflammatory activation of PBMCs in heart failure.
The study investigated the effect of boosting NAD levels on inflammatory activation in PBMCs of heart failure patients. Oral administration of nicotinamide riboside enhanced PBMC respiration and reduced proinflammatory cytokine gene expression in 4 subjects with heart failure.
Safety and Metabolism of Long-term Administration of NIAGEN (Nicotinamide Riboside Chloride) in a Randomized, Double-Blind, Placebo-controlled Clinical Trial of Healthy Overweight Adults.
An 8-week randomized, double-blind, placebo-controlled clinical trial evaluated the safety and metabolism of nicotinamide riboside (NR) in healthy overweight adults. NR significantly increased whole blood NAD levels dose-dependently without significant adverse events, supporting the development of a tolerable upper intake limit.
Repeat dose NRPT (nicotinamide riboside and pterostilbene) increases NAD+ levels in humans safely and sustainably: a randomized, double-blind, placebo-controlled study
Randomized, double-blind, placebo-controlled study of NRPT (nicotinamide riboside and pterostilbene) in 120 healthy adults aged 60-80. NRPT significantly increased NAD+ levels in a dose-dependent manner, with no serious adverse events reported. NAD+ levels increased by approximately 40% in the NRPT 1X group and 90% in the NRPT 2X group after 4 weeks, sustained throughout the 8-week trial.
Acute nicotinamide riboside supplementation increases human cerebral NAD+ levels in vivo
The study investigated the effect of acute nicotinamide riboside supplementation on cerebral NAD+ levels in the human brain using downfield proton MRS.
Nicotinamide riboside activates SIRT3 to prevent PTX-induced peripheral neuropathy.
The study investigates the role of nicotinamide riboside (NR) in preventing paclitaxel-induced peripheral neuropathy (CIPN) by activating SIRT3. NR protects dorsal root ganglion mitochondria against oxidative damage and enhances the anticancer activity of paclitaxel.
Protein Engineering of Nicotinamide Riboside Kinase Based on a Combinatorial Semirational Design Strategy for Efficient Biocatalytic Synthesis of Nicotinamide Mononucleotides.
The study focuses on protein engineering of nicotinamide riboside kinase to enhance the biocatalytic synthesis of β-nicotinamide mononucleotide (β-NMN). A semirational mutagenesis strategy was used to create a mutant with 2.9-fold higher enzyme activity, achieving high-density fermentation and optimizing an ATP-cycling whole-cell catalytic system for NMN production.
Long‐Term Nicotinamide Riboside Use Improves Coordination and Eye Movements in Ataxia Telangiectasia
The paper discusses the effects of long-term nicotinamide riboside supplementation on coordination and eye movements in ataxia telangiectasia, showing improvements in neurological outcomes.
NAD+-boosting therapy alleviates nonalcoholic fatty liver disease via stimulating a novel exerkine Fndc5/irisin
The study investigates the effects of NAD+-boosting therapy using nicotinamide riboside (NR) on nonalcoholic fatty liver disease (NAFLD) in humans and mice. NR treatment elevated Fndc5/irisin levels and showed therapeutic effects on NAFLD pathologies, which were compromised in Fndc5-/- mice. The study suggests that NR reverses NAFLD by regulating SIRT2-dependent Fndc5 deacetylation and deubiquitination.
In Ovo Feeding of Commercial Broiler Eggs: An Accurate and Reproducible Method to Affect Muscle Development and Growth.
The study developed a nicotinamide riboside in ovo feeding methodology for broiler-chicken embryos, which altered muscle development. Nicotinamide riboside increased pectoralis major muscle weight and muscle fiber density at hatch when injected into a developing embryo's yolk sac.
BST1 regulates nicotinamide riboside metabolism via its glycohydrolase and base-exchange activities
The study investigates the metabolic pathway of orally administered nicotinamide riboside (NR) and its role in increasing NAD+ levels. It identifies two pathways: the NR salvage pathway and the Preiss–Handler pathway, with BST1 playing a dual role as glycohydrolase and base-exchange enzyme.
Chemical and Biochemical Reactivity of the Reduced Forms of Nicotinamide Riboside.
The paper investigates the stability and degradation mechanisms of nicotinamide riboside (NRH) and its isomers, focusing on their chemical stability, reactivity, and cytotoxicity. It also explores NRH's role in nonenzymatic metabolism and potential for causing covalent peptide modifications, linking NADH accumulation to protein aging.
Results from a pilot study: The effects of nicotinamide riboside on mild cognitive impairment
Pilot study investigating the effects of nicotinamide riboside (NR) supplementation on brain function in older adults with mild cognitive impairment. NR is a precursor to NAD(+), which is important for cellular metabolism and cognitive function.
Effects of Nicotinamide Riboside on Endocrine Pancreatic Function and Incretin Hormones in Nondiabetic Men With Obesity.
A 12-week randomized, double-blind, placebo-controlled trial in 40 males with obesity and insulin resistance tested the effects of nicotinamide riboside (NR) supplementation on endocrine pancreatic function and incretin hormones. NR supplementation did not affect glucose tolerance, β-cell secretory capacity, α-cell function, incretin hormone secretion, or bile acid levels.
Nicotinamide riboside does not alter mitochondrial respiration, content or morphology in skeletal muscle from obese and insulin-resistant men.
This randomized, placebo-controlled clinical trial investigated the effects of nicotinamide riboside (NR) supplementation on skeletal muscle mitochondrial function in obese and insulin-resistant men. NR supplementation decreased nicotinamide phosphoribosyltransferase (NAMPT) protein abundance but did not affect NAD metabolite concentrations, mitochondrial respiration, content, or morphology.
Nicotinamide Riboside Augments the Aged Human Skeletal Muscle NAD+ Metabolome and Induces Transcriptomic and Anti-inflammatory Signatures
A placebo-controlled, randomized, double-blind, crossover trial with 12 aged men supplemented with 1 g NR per day for 21 days. NR elevated the muscle NAD+ metabolome and showed anti-inflammatory effects by depressing levels of circulating inflammatory cytokines. Muscle RNA sequencing revealed NR-mediated downregulation of energy metabolism and mitochondria pathways, without altering mitochondrial bioenergetics.
Nicotinamide riboside augments the human skeletal muscle NAD+ metabolome and induces transcriptomic and anti-inflammatory signatures in aged subjects: a placebo-controlled, randomized trial
Placebo-controlled, randomized, double-blind, crossover trial of 12 aged men supplemented with 1g/day nicotinamide riboside (NR) for 21 days. NR elevated the muscle NAD+ metabolome and showed anti-inflammatory effects, but did not show a difference in NAD+ levels between young and aged individuals in muscle and brain.
A randomized placebo-controlled clinical trial of nicotinamide riboside in obese men: safety, insulin-sensitivity, and lipid-mobilizing effects.
A randomized, placebo-controlled clinical trial tested the safety and effects of nicotinamide riboside (NR) supplementation on insulin sensitivity and metabolic parameters in 40 obese, insulin-resistant men over 12 weeks. NR supplementation was found to be safe but did not improve insulin sensitivity, glucose metabolism, or body composition.
Chronic nicotinamide riboside supplementation is well-tolerated and elevates NAD+ in healthy middle-aged and older adults
A 2x6-week randomized, double-blind, placebo-controlled, crossover clinical trial showed that chronic supplementation with nicotinamide riboside is well tolerated and effectively stimulates NAD+ metabolism in healthy middle-aged and older adults. The study suggests potential benefits for reducing blood pressure and arterial stiffness.
Fasting and refeeding differentially regulate NLRP3 inflammasome activation in human subjects.
Clinical study of 19 healthy volunteers undergoing a 24-hour fast followed by a fixed-calorie meal, analyzing NLRP3 inflammasome activation. An additional group of 8 volunteers assessed the effects of nicotinamide riboside on inflammasome activation. Fasting reduced NLRP3 activation, and SIRT3 activation blunted inflammasome activity, suggesting nutrient levels regulate inflammasome via mitochondrial control.
Effects of NAD+ supplementation with oral nicotinamide riboside on vascular health and cognitive function in older adults with peripheral artery disease: Results from a pilot 4-week open-label clinical trial.
Pilot 4-week open-label clinical trial of nicotinamide riboside (NR) supplementation in 8 older adults with peripheral artery disease (PAD). The study found positive trends in peripheral endothelial function, significant improvements in cerebrovascular response, and cognitive enhancement. NR was associated with reduced oxidative stress and increased mitochondrial efficiency in endothelial cells.
Vascular Endothelial NAMPT‐Mediated NAD + Biosynthesis Regulates Angiogenesis and Cardiometabolic Functions in Male Mice
The study investigates the role of NAMPT in NAD+ biosynthesis and its impact on vascular endothelial function and cardiometabolic health in aging male mice. Nicotinamide mononucleotide administration improved angiogenesis, restored adipose tissue volume, and ameliorated cardiometabolic dysfunction in aged mice.
Nicotinamide riboside supplementation ameliorates ovarian dysfunction in a PCOS mouse model
The study investigated the effects of nicotinamide riboside supplementation on ovarian dysfunction in a PCOS mouse model. Supplementation prevented the decrease in ovarian NAD+ levels, normalized estrous cycle irregularities, enhanced ovulation potential, alleviated ovarian fibrosis, and improved oocyte quality and mitochondrial function in PCOS mice.
Nicotinamide riboside alleviates sweeteners-induced brain and cognitive impairments in immature mice.
The study evaluated the effects of nicotinamide riboside (NR) on cognitive impairments induced by sweeteners in immature mice. NR supplementation increased NAD+ in the brain and alleviated cognitive impairments, neuroinflammation, and oxidative stress caused by sucrose or aspartame exposure.
Nicotinamide Riboside: A Promising Treatment for Type 1 Cardiorenal Syndrome in Myocardial Infarction‐Induced Acute Kidney Injury by Upregulating Nicotinamide Phosphoribosyltransferase‐Mediated Nicotinamide Dinucleotide Levels
The study investigated the effects of nicotinamide riboside (NR) on myocardial infarction-induced acute kidney injury in mice. NR treatment did not significantly improve cardiac ejection fraction but enhanced kidney function and reduced morphological alterations and cell death, suggesting renal protection through upregulation of NAMPT-mediated increase in renal NAD levels.
Decreased mitochondrial NAD+ in WRN deficient cells links to dysfunctional proliferation
The study investigates the effects of nicotinamide riboside supplementation on mitochondrial NAD+ levels and senescence in WRN-deficient mesenchymal stem cells and fibroblasts from Werner syndrome patients. Nicotinamide riboside reduces senescence and improves mitochondrial metabolic profiles, although it does not restore proliferation in WRN knockdown cells.
Nicotinamide Riboside Mitigates Retinal Degeneration by Suppressing Damaged DNA-Stimulated Microglial Activation and STING-Mediated Pyroptosis
The study investigates the effects of nicotinamide riboside (NR) on retinal degeneration in Balb/c mice exposed to bright light. NR treatment preserved mitochondrial biosynthesis, inhibited STING expression in reactive microglia, and reduced the pro-inflammatory response, suggesting neuroprotection by attenuating STING-mediated microglial activation and pyroptosis.
Nicotinamide Riboside Supplementation Alleviates Testicular Aging Induced by Disruption of Qprt‐Dependent NAD + De Novo Synthesis in Mice
The study investigated the impact of NAD+ de novo synthesis on spermatogenesis in Qprt-deficient mice. Nicotinamide riboside supplementation restored NAD+ levels and rescued spermatogenic defects, highlighting the importance of NAD+ homeostasis in spermatogenesis.
Nicotinamide riboside and nicotinamide mononucleotide facilitate NAD+ synthesis via enterohepatic circulation
The study analyzed the metabolic pathways of orally and intravenously administered nicotinamide mononucleotide (NMN) and nicotinamide riboside (NR) in mice using NAD+ metabolomics. It found that NMN and NR are mostly converted to nicotinic acid by gut microbiota and then used in the liver, indicating indirect conversion to NAD+ via unexpected pathways.
Ability of Nicotinamide Riboside to Prevent Muscle Fatigue of Barrows Subjected to a Performance Test
The study investigated the effect of dietary nicotinamide riboside (NR) on muscle fatigue in barrows. Barrows were assigned to different NR doses and tested for muscle fatigue. The 15NR and DRE treatments showed greater distances to exhaustion compared to the control, indicating NR's potential in fatigue prevention.
Nicotinamide riboside restores nicotinamide adenine dinucleotide levels and alleviates brain injury by inhibiting oxidative stress and neuroinflammation in a mouse model of intracerebral hemorrhage
The study investigates the protective effects of nicotinamide riboside against collagenase-induced hemorrhagic stroke in a mouse model. Nicotinamide riboside reduced hemorrhagic area, cerebral water content, and histopathological damage, promoted neurological recovery, and suppressed oxidative stress and neuroinflammation.
Nicotinamide Riboside Ameliorates Hyperpigmentation on Photo-Irradiated Skin
In vitro study investigating the effects of nicotinamide riboside (NR) on hyperpigmentation in photo-irradiated skin. NR inhibited melanogenesis and tyrosinase activity in B16F10 cells and reduced metalloproteinase gene expression in UVB-stimulated HaCaT cells, suggesting potential as a skin-whitening ingredient.
Nicotinamide ribose ameliorates myocardial ischemia/reperfusion injury by regulating autophagy and regulating oxidative stress
The study investigates the protective effects of nicotinamide riboside (NR) on myocardial ischemia-reperfusion injury in a mouse model. NR supplementation reduced myocardial ischemic infarction, alleviated pathological changes, decreased inflammatory cell infiltration, and attenuated oxidative stress. The study suggests NR activates sirt 1 via NAD+ upregulation, protecting against autophagy.
NAD+ precursors prolong survival and improve cardiac phenotypes in a mouse model of Friedreich’s Ataxia
The study investigates the effects of NAD+ precursors, specifically nicotinamide mononucleotide and riboside, on a mouse model of Friedreich’s Ataxia with systemic frataxin knockdown. Administration of these precursors increased survival, modestly improved cardiac hypertrophy, and enhanced antioxidant capacity, suggesting modest cardioprotective effects.
Nicotinamide riboside modulates the reactive species interactome, bioenergetic status and proteomic landscape in a brain-region-specific manner.
The study investigates the effects of nicotinamide riboside (NR) on the reactive species interactome, bioenergetic status, and proteomic landscape in brain regions of AD mouse models. NR showed cognitive benefits and alleviated neuroinflammation without affecting beta-amyloid plaque pathology, with brain-area specific effects on oxidative phosphorylation, fatty acid oxidation, and neurotransmitter regulation pathways.
Insulin and glycolysis dependency of cardioprotection by nicotinamide riboside
The study investigates the cardioprotective effects of nicotinamide riboside (NR) against ischemia-reperfusion injury (IRI) in isolated mouse hearts. NR increased cardiac NAD+ levels and provided protection in conditions of low glycolysis, but this protection was lost in the presence of insulin or when glycolysis was not activated.
Cytochrome b 5 reductase 3 overexpression and dietary nicotinamide riboside supplementation promote distinctive mitochondrial alterations in distal convoluted tubules of mouse kidneys during aging
The study investigates the effects of cytochrome b5 reductase 3 overexpression and dietary nicotinamide riboside supplementation on mitochondrial alterations in distal convoluted tubules of mouse kidneys during aging. The interventions showed differential effects based on age and sex, with nicotinamide riboside mitigating age-related changes in males and transgenesis in females.
The NAD+ precursor nicotinamide riboside protects against postovulatory aging in vitro
The study investigates the effects of nicotinamide riboside (NR) on postovulatory aging (POA) in vitro using mouse oocytes. Supplementation with 200 μM NR for 24 hours reduced signs of aging-related decline, including ROS accumulation and mitochondrial dysfunction, and improved spindle assembly and chromosome alignment, potentially enhancing embryonic development potential.
Chronically Low NMNAT2 Expression Causes Sub-lethal SARM1 Activation and Altered Response to Nicotinamide Riboside in Axons
The study investigates the effects of chronically low NMNAT2 expression on SARM1 activation and the response to nicotinamide riboside (NR) in axons. It shows that low NMNAT2 levels lead to sub-lethal SARM1 activation and reverse the NAD-enhancing effect of NR in a SARM1-dependent manner, suggesting potential implications for human axonal disorders.
The Effectiveness of Four Nicotinamide Adenine Dinucleotide (NAD+) Precursors in Alleviating the High-Glucose-Induced Damage to Hepatocytes in Megalobrama amblycephala: Evidence in NAD+ Homeostasis, Sirt1/3 Activation, Redox Defense, Inflammatory Response, Apoptosis, and Glucose Metabolism
The study compared the effectiveness of four NAD+ precursors (NA, NAM, NR, NMN) in alleviating high-glucose-induced damage to hepatocytes in a fish model. Nicotinamide riboside (NR) showed the most potent effects in improving cell viability, boosting NAD+ levels, activating Sirt1/3, and benefiting glucose metabolism, compared to other precursors.
NAD+ Precursors Reverse Experimental Diabetic Neuropathy in Mice
The study investigated the effects of NAD+ precursors, Nicotinamide Riboside (NR) and Nicotinamide Mononucleotide (NMN), on diabetic peripheral neuropathy in mice. NR and NMN supplementation increased NAD+ levels and SIRT1 activity, reversing neuropathy symptoms and restoring nerve function in diabetic mice.
Nicotinamide Riboside and CD38: Covalent Inhibition and Live-Cell Labeling
The study explores the inhibition potential of nicotinamide riboside (NR) against CD38, a major degrader of NAD+. NR exhibits marked inhibitory activity by forming a stable ribosyl–ester bond with CD38, offering new insights into NR's role in modulating NAD+ metabolism and CD38-mediated signaling.
Nicotinamide Riboside-Driven Modulation of SIRT3/mtROS/JNK Signaling Pathways Alleviates Myocardial Ischemia-Reperfusion Injury
The study investigates the cardioprotective effects of Nicotinamide Riboside (NR) in mice with myocardial ischemia-reperfusion injury. NR improved heart function, reduced cardiomyocyte apoptosis, and modulated the SIRT3/mtROS/JNK pathway, suggesting its potential as a therapeutic agent for ischemic heart diseases.
Protective Effect of Nicotinamide Riboside on Glucocorticoid-Induced Glaucoma: Mitigating Mitochondrial Damage and Extracellular Matrix Deposition
The study investigates the effects of nicotinamide riboside (NR) on glucocorticoid-induced glaucoma (GIG) using in vitro and in vivo models. NR significantly prevented ECM-related protein expression and dysfunction in human TM cells and protected against mitochondrial damage in GIG models. In mice, NR partially prevented IOP elevation and RGC loss, suggesting its potential as a therapeutic candidate for GIG.
The Role of Nicotinamide Riboside in the Preservation of Retinal Ganglion Cells Using An In Vitro Glutamate-Induced Excitotoxicity Model.
The study investigates the role of nicotinamide riboside (NR) in preserving retinal ganglion cells (RGCs) using an in vitro glutamate-induced excitotoxicity model. NR treatment prevented cell viability decrease, inhibited apoptosis, and attenuated oxidative stress in R28 cells. It also elevated NAD+ levels and restored protein expression of SIRT1 and PGC1α, suggesting a protective effect mediated through the SIRT1/PGC1α pathway.
Comparison of protective effects of nicotinamide mononucleotide and nicotinamide riboside on DNA damage induced by cisplatin in HeLa cells
The study compared the protective effects of nicotinamide mononucleotide (NMN) and nicotinamide riboside (NR) on cisplatin-induced DNA damage in HeLa cells. Both NMN and NR protected against DNA damage and boosted DNA repair, with NR showing a better protective effect. Both substances increased cellular NAD+ levels and inhibited cisplatin-induced ROS increase.
Nicotinamide Riboside Ameliorates Fructose-Induced Lipid Metabolism Disorders in Mice by Activating Browning of WAT, and May Be Also Related to the Regulation of Gut Microbiota
The study investigates the effect of nicotinamide riboside (NR) on fructose-induced lipid metabolism disorders in mice. NR supplementation reduced body weight, liver weight, white adipose tissue weight, and lipid levels, and upregulated proteins related to browning of white adipose tissue. It also enhanced certain gut microbiota, suggesting a role in modulating intestinal flora.
Hepatic steatosis induced by nicotine plus Coca-Cola™ is prevented by nicotinamide riboside (NR)
The study evaluated the effects of nicotine plus Coca-Cola™ on hepatic steatosis in mice and the protective role of nicotinamide riboside (NR). Nic+Coke increased caloric intake and induced hepatic steatosis, while NR supplementation prevented these changes by reducing oxidative stress and preventing mitochondrial damage.
Nicotinamide riboside targets mitochondrial unfolded protein response to reduce alcohol‐induced damage in Kupffer cells
The study investigates the effects of nicotinamide riboside (NR) on ethanol-induced damage in Kupffer cells, focusing on the mitochondrial unfolded protein response (UPRmt). NR was found to counteract ethanol-induced inflammation, oxidative stress, and mitochondrial dysfunction in immortalized mouse Kupffer cells by modulating the ATF5-dependent UPRmt pathway.
Novel Approach to Skin Anti-Aging: Boosting Pharmacological Effects of Exogenous Nicotinamide Adenine Dinucleotide (NAD+) by Synergistic Inhibition of CD38 Expression
In vitro study on human fibroblasts demonstrating that exogenous NAD+ can exert protective effects against skin aging, both extrinsic and intrinsic. The effects were enhanced by quercetin and enoxolone through inhibition of CD38, improving sirtuin activation, autophagy, and mitochondrial functionality.
The Role and Mechanism of Nicotinamide Riboside in Oxidative Damage and a Fibrosis Model of Trabecular Meshwork Cells
The study investigates the effects of nicotinamide riboside (NR) on oxidative stress and fibrosis in human trabecular meshwork cells. NR pretreatment improved cell viability, proliferation, and mitochondrial membrane potential, reduced apoptosis and ROS generation, and inhibited fibrotic responses induced by TGF-β2. The protective effects may involve the JAK2/Stat3 and MAPK pathways.
Nicotinamide Mononucleotide and Nicotinamide Riboside Reverse Ovarian Aging in Rats Via Rebalancing Mitochondrial Fission and Fusion Mechanisms
This study examined the effects of nicotinamide mononucleotide (NMN) and nicotinamide riboside (NR) on folliculogenesis and mitochondrial dynamics in ovaries of middle-aged female rats. NMN and NR were found to rebalance mitochondrial dynamics, decrease excessive fission, alleviate mitochondrial stress, and correct aging-induced folliculogenesis abnormalities.
Nicotinamide riboside attenuates myocardial ischemia-reperfusion injury via regulating SIRT3/SOD2 signaling pathway.
The study investigated the effects of nicotinamide riboside (NR) on myocardial ischemia-reperfusion injury in rats. Pretreatment with NR significantly reduced myocardial infarct area, decreased serum CK-MB and LDH levels, and improved cardiac function. NR also increased cell viability and decreased LDH release in H9c2 cells, suggesting a protective role via the SIRT3-SOD2 signaling pathway.
Effect of Nicotinamide Riboside Against the Exhaustion of CD8+ T Cells via Alleviating Mitochondrial Dysfunction
In vitro study investigating the effect of nicotinamide riboside (NR) on CD8+ T cell exhaustion and mitochondrial function. NR supplementation inhibited CD8+ T cell exhaustion, preserved mitochondrial function, and reduced apoptosis, with effects linked to the SirT1 pathway.
WRN loss accelerates abnormal adipocyte metabolism in Werner syndrome
The study investigates the role of WRN loss in adipocyte metabolism in Werner syndrome using in vitro stem cells and in vivo zebrafish models. Nicotinamide riboside supplementation was found to restore adipocyte metabolism, suggesting a potential therapeutic approach for metabolic dysfunction in WS.
Nicotinamide riboside improves muscle mitochondrial biogenesis, satellite cell differentiation, and gut microbiota in a twin study
Study on 20 BMI-discordant monozygotic twin pairs supplemented with nicotinamide riboside (NR) for 5 months. NR improved systemic NAD+ metabolism, muscle mitochondrial number, myoblast differentiation, and gut microbiota composition, but did not ameliorate adiposity or metabolic health.
Epithelial NAD+ depletion drives mitochondrial dysfunction and contributes to intestinal inflammation
The study investigates the effects of nicotinamide riboside on intestinal inflammation and mitochondrial function in mice with experimental colitis. Treatment with nicotinamide riboside reduced colitis severity, restored mitochondrial function, and increased active PGC1α levels, suggesting a potential therapeutic approach for inflammatory bowel disease.
The NAD salvage pathway in mesenchymal cells is indispensable for skeletal development in mice
The study investigates the role of the NAD salvage pathway in mesenchymal cells for skeletal development in mice. Deletion of Nampt in mesenchymal lineage cells leads to limb shortening due to chondrocyte death, but administration of nicotinamide riboside during pregnancy prevents most in utero defects, highlighting the importance of NAD homeostasis in bone formation.
Nicotinamide riboside intervention alleviates hematopoietic system injury of ionizing radiation‐induced premature aging mice
The study evaluated the effects of nicotinamide riboside on premature aging in mice induced by ionizing radiation. Nicotinamide riboside treatment reversed aging spleen phenotypes, ameliorated serum metabolism profiles, and alleviated the myeloid bias of hematopoietic stem cells, suggesting its potential as a therapeutic agent for radiation-exposed populations.
Nicotinamide Riboside Preserves Ovarian Injury in an Experimental Sepsis Model in Rats
The study investigated the protective effects of nicotinamide riboside on oxidative stress in an experimental sepsis model in rats. Nicotinamide riboside treatment increased superoxide dismutase activity and decreased myeloperoxidase activities and malondialdehyde concentration in ovarian tissue, reducing inflammation and follicular damage.
The NAD+ Precursor Nicotinamide Riboside Rescues Mitochondrial Defects and Neuronal Loss in iPSC derived Cortical Organoid of Alpers' Disease
The study used iPSC-derived cortical organoids from an Alpers' disease patient to investigate neural dysfunction. Nicotinamide riboside significantly ameliorated mitochondrial defects in patient brain organoids, suggesting its potential as a therapeutic candidate for Alpers' disease.
Raising NAD+ Level Stimulates Short-Chain Dehydrogenase/Reductase Proteins to Alleviate Heart Failure Independent of Mitochondrial Protein Deacetylation
The study investigated the effects of nicotinamide riboside chloride on heart failure in SIRT3-deficient and wild-type mice. Nicotinamide riboside chloride improved mitochondrial function and reduced heart failure progression, suggesting benefits of NAD+-based therapy in pre-existing heart failure independent of Sirt3-mediated pathways.
SUPPLEMENTATION WITH NICOTINAMIDE RIBOSIDE ATTENUATES T CELL EXHAUSTION AND IMPROVES SURVIVAL IN SEPSIS
Animal study using a septic model to investigate the effects of nicotinamide riboside (NR) supplementation. NR increased NAD+ and SIRT1 levels, alleviated T cell exhaustion, reduced bacterial load, organ damage, and mortality in septic mice, demonstrating a beneficial effect on sepsis and T cell function.
Nicotinamide Riboside, an NAD + Precursor, Protects Against Cardiac Mitochondrial Dysfunction in Fetal Guinea Pigs Exposed to Gestational Hypoxia
The study tested nicotinamide riboside (NR) as a treatment for mitochondrial dysfunction in hypoxic fetal guinea pig hearts. NR prevented hypoxia-induced reductions in fetal body weight and normalized mitochondrial function and ATP synthesis in the fetal heart.
Complex I deficiency in m.3243A>G fibroblasts is alleviated by reducing NADH accumulation
The study investigated the effects of nicotinamide riboside (NR) and mitochondria-targeted H2O-forming NADH oxidase (mitoLbNOX) on fibroblasts with the m.3243A>G mutation. NR treatment increased the NAD+/NADH ratio, complex I-dependent OCR, and ATP levels, while mitoLbNOX treatment further improved these parameters and reduced NADH levels.
Nicotinamide riboside rescues dysregulated glycolysis and fatty acid β-oxidation in a human hepatic cell model of citrin deficiency
The study used a CITRIN knockout HepG2 cell line to model citrin deficiency and found that nicotinamide riboside normalized glycolysis and fatty acid oxidation. However, it did not affect hyperammonemia, suggesting a potential strategy to treat some metabolic defects of citrin deficiency.
Nicotinamide riboside activates SIRT5 deacetylation
The study identifies nicotinamide riboside (NR) as a SIRT5-selective allosteric activator, increasing SIRT5 catalytic efficiency with synthetic peptide substrates. The research explores the mechanism of action and maps the NR binding site, suggesting potential therapeutic applications for metabolic disorders and age-related diseases.
Axonal Protection by Oral Nicotinamide Riboside Treatment with Upregulated AMPK Phosphorylation in a Rat Glaucomatous Degeneration Model
The study investigated the effects of oral nicotinamide riboside (NR) on axonal damage in a rat ocular hypertension model. Oral NR administration significantly increased NAD+ levels in the retina and protected against retinal ganglion cell fiber loss and axonal degeneration, potentially through upregulation of phosphorylated AMPK.