Research

Lactobacillus salivarius

31 peer-reviewed studies curated from PubMed and Semantic Scholar.

1
Meta-analyses
2
Systematic reviews
27
RCTs
1
Other studies
Meta-analyses (3%)
Systematic reviews (6%)
RCTs (87%)

Studies

Sorted by quality and recency

2025·Nutrition journal·Ming-Luen Tsai, Shang-Po Shen, Yin-Ting Chen, et al

Effects of phototherapy combined with Lactobacillus salivarius AP-32 or Bifidobacterium animalis subsp. lactis CP-9 on improving neonatal jaundice and gut microbiome health: a randomized double-blind clinical study.

RCTn = 300Gut Health Nutrition

RCT involving 300 full-term infants with neonatal jaundice, comparing phototherapy combined with probiotic supplementation of Lactobacillus salivarius AP-32 or Bifidobacterium animalis subsp. lactis CP-9. Both probiotic groups reduced phototherapy duration and accelerated bilirubin reduction. The AP-32 group also showed reduced hospitalization duration and increased gut microbial diversity.

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2023·Clinical and experimental dermatology·Husein Husein-ElAhmed, Martin Steinhoff

Effects of probiotic supplementation in adult with atopic dermatitis: a systematic review with meta-analysis.

Meta-analysisSkin Hair Health

Systematic review and meta-analysis of probiotic supplementation in adults with atopic dermatitis. Probiotics, particularly Lactobacillus salivarius and L. acidophilus, showed significant improvement in AD severity, especially in moderate-to-severe cases. No significant changes were observed in IgE levels and eosinophil count.

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2025·Journal of midwifery & women's health·Katrina Nardini, Lisa Hanson, Noelle Borders, et al

Open-Label Randomized Controlled Trial and Feasibility Study of an Oral Probiotic Intervention to Reduce Group B Streptococcus Colonization in Pregnant People by the Time of Birth.

RCTn = 68Womens Health

Open-label RCT assessing the feasibility of using Florajen Digestion, an oral probiotic, to reduce Group B Streptococcus colonization in pregnant individuals. The study enrolled 68 participants, with 46 completing intrapartum cultures. A larger decrease in GBS colonization was noted among probiotic users, though the study was not powered to detect significant differences. No adverse events were reported.

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2024·PloS one·Pamella de Barros Motta, Marcela Leticia Leal Gonçalves, Juliana Maria Altavista Sagretti Gallo, et al

Short term effect of antimicrobial photodynamic therapy and probiotic L. salivarius WB21 on halitosis: A controlled and randomized clinical trial.

RCTn = 52

This randomized controlled trial evaluated the effect of antimicrobial photodynamic therapy (aPDT) and probiotics on halitosis in 52 participants. The study found an immediate significant reduction in halitosis with aPDT and tongue scraping, but probiotics showed no difference in relation to initial levels. No significant changes were observed in bacterial counts.

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2010·The Journal of nutrition·Stephen J Allen, Susan Jordan, Melanie Storey, et al

Dietary supplementation with lactobacilli and bifidobacteria is well tolerated and not associated with adverse events during late pregnancy and early infancy.

RCTn = 454

RCT evaluating the safety of a bacterial dietary supplement containing lactobacilli and bifidobacteria for the prevention of atopy in infants. The study found no significant difference in adverse events between the treatment and placebo groups, supporting the safe use of these organisms during pregnancy and early infancy.

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2000·The Cochrane database of systematic reviews·W Sandborn, R McLeod, D Jewell

Pharmacotherapy for inducing and maintaining remission in pouchitis.

Systematic review

Systematic review evaluating the effectiveness of various medical therapies, including metronidazole, bismuth carbomer enemas, oral probiotic bacteria (VSL-3), butyrate suppositories, and glutamine suppositories, for inducing response or maintaining remission in pouchitis. Metronidazole and VSL-3 probiotics appear effective for active chronic pouchitis and maintaining remission, respectively. Bismuth carbomer enemas and glutamine versus butyrate suppositories showed no significant differences in efficacy.

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2021·Current microbiology·Wen-Yang Lin, Yi-Wei Kuo, Ching-Wei Chen, et al

Viable and Heat-Killed Probiotic Strains Improve Oral Immunity by Elevating the IgA Concentration in the Oral Mucosa.

RCTn = 45Immunity

RCT with 45 healthy participants testing the effects of viable and heat-killed probiotic tablets on oral immunity. Both forms increased salivary IgA levels and reduced Streptococcus mutans. Lactobacillus salivarius AP-32 increased TGF-beta and IL-10 levels in PBMCs.

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2018·Probiotics and antimicrobial proteins·Neda Sajedinejad, Mojgan Paknejad, Behzad Houshmand, et al

Lactobacillus salivarius NK02: a Potent Probiotic for Clinical Application in Mouthwash.

RCTInflammation Gut Health

A randomized double-blind placebo-controlled trial tested a mouthwash containing Lactobacillus salivarius NK02 for periodontitis treatment. The probiotic mouthwash inhibited bacterial growth and reduced gingival index, bleeding on probing, and pocket depth, suggesting benefits for dental and periodontal health.

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2016·Biomedical papers of the Medical Faculty of the University Palacky, Olomouc, Czechoslovakia·Vincenzo Davide Palumbo, Marcello Romeo, Antonella Marino Gammazza, et al

The long-term effects of probiotics in the therapy of ulcerative colitis: A clinical study.

RCTn = 60Gut Health

RCT evaluating the long-term effects of a combination therapy of mesalazine and a probiotic blend on ulcerative colitis activity in 60 patients. The combination therapy showed better improvement compared to mesalazine alone, with beneficial effects of probiotics evident after two years.

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2015·American journal of obstetrics and gynecology·Karen L Lindsay, Lorraine Brennan, Maria A Kennelly, et al

Impact of probiotics in women with gestational diabetes mellitus on metabolic health: a randomized controlled trial.

RCTn = 149Womens Health -Heart Health

This double-blind placebo-controlled randomized trial investigated the effects of a probiotic capsule (Lactobacillus salivarius UCC118) on metabolic parameters in pregnant women with gestational diabetes. The study found no differences in postintervention fasting glucose or requirement for pharmacological therapy between the probiotic and placebo groups. However, the probiotic group showed an attenuation of the rise in total and LDL cholesterol compared to placebo.

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2015·Clinical infectious diseases : an official publication of the Infectious Diseases Society of America·Leónides Fernández, Nivia Cárdenas, Rebeca Arroyo, et al

Prevention of Infectious Mastitis by Oral Administration of Lactobacillus salivarius PS2 During Late Pregnancy.

RCTn = 108Womens Health Immunity

RCT evaluating the prevention of infectious mastitis by oral administration of Lactobacillus salivarius PS2 during late pregnancy. The probiotic group had a significantly lower incidence of mastitis (25%) compared to the placebo group (57%), with lower milk bacterial counts when mastitis occurred.

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2014·Pharmacological research·Rosa Vázquez-Fresno, Rafael Llorach, Jelena Marinic, et al

Urinary metabolomic fingerprinting after consumption of a probiotic strain in women with mastitis.

RCT

The study applied a (1)H NMR-based metabolomic approach to detect metabolomic differences after consuming Lactobacillus salivarius PS2 in women with mastitis. Urinary metabolomic fingerprinting showed differences after 21 days of probiotic administration, with a reduction in the use of ibuprofen and acetaminophen observed.

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2013·Clinical nutrition (Edinburgh, Scotland)·Nadja Larsen, Finn K Vogensen, Rikke Juul Gøbel, et al

Effect of Lactobacillus salivarius Ls-33 on fecal microbiota in obese adolescents.

RCTn = 50Gut Health

This double-blinded RCT investigated the impact of Lactobacillus salivarius Ls-33 on fecal microbiota in 50 obese adolescents over 12 weeks. The study found significant changes in the ratios of certain bacterial groups but no significant changes in overall fecal bacteria cell numbers or short chain fatty acids.

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2013·Oral surgery, oral medicine, oral pathology and oral radiology·Nao Suzuki, Masahiro Yoneda, Kazunari Tanabe, et al

Lactobacillus salivarius WB21--containing tablets for the treatment of oral malodor: a double-blind, randomized, placebo-controlled crossover trial.

RCT

A 14-day double-blind, placebo-controlled, randomized crossover trial evaluated the effect of Lactobacillus salivarius WB21 tablets on oral malodor. The probiotic period showed significant decreases in volatile sulfur compounds and probing pocket depth compared to placebo, suggesting potential benefits in controlling oral malodor.

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2012·Indian pediatrics·Teng-Yi Lin, Chia-Jung Chen, Li-Kuang Chen, et al

Effect of probiotics on allergic rhinitis in Df, Dp or dust-sensitive children: a randomized double blind controlled trial.

RCTn = 199Immunity

RCT at Hualien Tzu-Chi General Hospital studied the effect of Lactobacillus salivarius on allergic rhinitis in children. The probiotic reduced rhinitis symptoms and drug usage, but no significant differences were found in immunological or blood cell variables between the probiotic and placebo groups.

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2012·International journal of sport nutrition and exercise metabolism·Michael Gleeson, Nicolette C Bishop, Marta Oliveira, et al

Effects of a Lactobacillus salivarius probiotic intervention on infection, cold symptom duration and severity, and mucosal immunity in endurance athletes.

RCTn = 66Immunity -

RCT of 66 endurance athletes examining the effects of Lactobacillus salivarius probiotic supplementation on upper respiratory tract infections and mucosal immunity. The study found no significant differences in infection incidence, symptom severity, or immune markers between the probiotic and placebo groups.

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2012·Journal of pediatric gastroenterology and nutrition·Rikke Juul Gøbel, Nadja Larsen, Mogens Jakobsen, et al

Probiotics to adolescents with obesity: effects on inflammation and metabolic syndrome.

RCTn = 50Inflammation -Nutrition -

Double-blind placebo-controlled trial of Lactobacillus salivarius Ls-33 in 50 adolescents with obesity over 12 weeks. No beneficial effects were detected on inflammatory markers or parameters related to metabolic syndrome.

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2011·The British journal of dermatology·K-G Wu, T-H Li, H-J Peng

Lactobacillus salivarius plus fructo-oligosaccharide is superior to fructo-oligosaccharide alone for treating children with moderate to severe atopic dermatitis: a double-blind, randomized, clinical trial of efficacy and safety.

RCTn = 60Skin Hair Health

Double-blind RCT comparing synbiotic (Lactobacillus salivarius plus fructo-oligosaccharide) to prebiotic (fructo-oligosaccharide alone) in 60 children with moderate to severe atopic dermatitis. The synbiotic group showed significantly lower SCORAD scores and reduced AD intensity at 8 weeks, with decreased medication use and eosinophil cationic protein levels.

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2009·Nutrition (Burbank, Los Angeles County, Calif.)·José Maldonado, Federico Lara-Villoslada, Saleta Sierra, et al

Safety and tolerance of the human milk probiotic strain Lactobacillus salivarius CECT5713 in 6-month-old children.

RCTn = 80Gut Health

A double-blinded, randomized, placebo-controlled study evaluated the safety of a follow-on formula with Lactobacillus salivarius CECT5713 in 6-month-old children. The probiotic formula was safe, well-tolerated, and increased fecal lactobacilli content and butyric acid concentration.

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2009·Journal of clinical periodontology·Gen Mayanagi, Moto Kimura, Seigo Nakaya, et al

Probiotic effects of orally administered Lactobacillus salivarius WB21-containing tablets on periodontopathic bacteria: a double-blinded, placebo-controlled, randomized clinical trial.

RCTn = 66

RCT of 66 healthy volunteers evaluating the effect of Lactobacillus salivarius WB21 on periodontopathic bacteria. The probiotic group showed a significant reduction in the numerical sum of five selected periodontopathic bacteria in subgingival plaque, suggesting beneficial effects on periodontal conditions.

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2008·Journal of clinical periodontology·Hidetoshi Shimauchi, Gen Mayanagi, Seigo Nakaya, et al

Improvement of periodontal condition by probiotics with Lactobacillus salivarius WB21: a randomized, double-blind, placebo-controlled study.

RCTn = 66

This randomized, double-blind, placebo-controlled study evaluated the effect of Lactobacillus salivarius WB21 on periodontal condition in 66 volunteers. The probiotic group showed significant improvements in plaque index and probing pocket depth, especially among smokers, compared to the placebo group.

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1999·Inflammatory bowel diseases·W J Sandborn, R McLeod, D P Jewell

Medical therapy for induction and maintenance of remission in pouchitis: a systematic review.

Systematic review

Systematic review evaluating the effectiveness of various medical therapies, including metronidazole, bismuth carbomer enemas, oral probiotic bacteria (VSL-3), butyrate suppositories, and glutamine suppositories, for inducing response or maintaining remission in pouchitis. Metronidazole and VSL-3 were found effective for active chronic pouchitis and maintaining remission, respectively, while bismuth carbomer enemas were not effective.

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2019·Journal of periodontology·Camila Rafaela Mousquer, Alvaro Della Bona, Daiane Cristina Milani, et al

Are Lactobacillus salivarius G60 and inulin more efficacious to treat patients with oral halitosis and tongue coating than the probiotic alone and placebo? A randomized clinical trial.

RCTn = 45

This randomized clinical trial assessed the effect of Lactobacillus salivarius G60 and inulin on oral halitosis and tongue coating in 45 patients. The combination of LS and inulin showed a greater reduction in halitosis compared to placebo, with improvements in quality of life. LS inhibited the growth of P. gingivalis and P. intermedia but did not metabolize inulin.

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2014·Journal of cardiovascular pharmacology and therapeutics·Hemalatha Rajkumar, Manoj Kumar, Nilita Das, et al

Effect of Probiotic Lactobacillus salivarius UBL S22 and Prebiotic Fructo-oligosaccharide on Serum Lipids, Inflammatory Markers, Insulin Sensitivity, and Gut Bacteria in Healthy Young Volunteers: A Randomized Controlled Single-Blind Pilot Study.

RCTn = 45Heart Health Inflammation Gut Health

This randomized controlled single-blind pilot study investigated the effect of 6-week supplementation of Lactobacillus salivarius UBL S22 with or without fructo-oligosaccharide (FOS) on serum lipids, inflammatory markers, insulin sensitivity, and gut bacteria in 45 healthy young individuals. Significant improvements in cholesterol profiles, reduction in inflammatory markers, and beneficial changes in gut bacteria were observed, with more pronounced effects in the synbiotic group.

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2014·Journal of clinical gastroenterology·Lorenzo Drago, Elena De Vecchi, Marco Toscano, et al

Treatment of atopic dermatitis eczema with a high concentration of Lactobacillus salivarius LS01 associated with an innovative gelling complex: a pilot study on adults.

RCTn = 25Skin Hair Health

A prospective, controlled pilot trial evaluated the efficacy of a high concentration of Lactobacillus salivarius LS01 with a gelling complex of Streptococcus thermophilus ST10 and tara gum in treating atopic dermatitis in adults. The probiotic group showed significant improvement in the SCORAD index after 1 month, with a slight decrease in fecal S. aureus count.

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2012·Journal of breath research·Nao Suzuki, Kazunari Tanabe, Toru Takeshita, et al

Effects of oil drops containing Lactobacillus salivarius WB21 on periodontal health and oral microbiota producing volatile sulfur compounds.

RCTn = 42

RCT evaluating the effects of oil drops containing Lactobacillus salivarius WB21 on periodontal health and oral microbiota in 42 subjects. The experimental group showed reduced bleeding on probing and inhibited reproduction of VSC-producing bacteria compared to placebo.

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2012·Journal of clinical gastroenterology·Lorenzo Drago, Marco Toscano, Elena De Vecchi, et al

Changing of fecal flora and clinical effect of L. salivarius LS01 in adults with atopic dermatitis.

RCTn = 38Skin Hair Health Immunity

RCT of 38 adults with moderate/severe atopic dermatitis comparing L. salivarius LS01 probiotic to placebo. The probiotic group showed reduced SCORAD scores, decreased staphylococci load, and reduced Th2 cytokine production, indicating positive effects on clinical and immunologic status.

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2011·International journal of immunopathology and pharmacology·L Drago, E Iemoli, V Rodighiero, et al

Effects of Lactobacillus salivarius LS01 (DSM 22775) treatment on adult atopic dermatitis: a randomized placebo-controlled study.

RCTn = 38Skin Hair Health

This randomized, double-blind, placebo-controlled study evaluated the efficacy of Lactobacillus salivarius LS01 in treating adult atopic dermatitis. Patients treated with the probiotic showed significant improvements in SCORAD and DLQI scores compared to placebo. The probiotic also modulated Th1/Th2 cytokine profiles and decreased staphylococci in feces.

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2010·Oral surgery, oral medicine, oral pathology, oral radiology, and endodontics·Tomoyuki Iwamoto, Nao Suzuki, Kazunari Tanabe, et al

Effects of probiotic Lactobacillus salivarius WB21 on halitosis and oral health: an open-label pilot trial.

RCTn = 20

Open-label pilot trial evaluating the effects of Lactobacillus salivarius WB21 on halitosis and oral health in 20 patients. Oral malodor parameters significantly decreased at 2 weeks, and organoleptic test scores and bleeding on probing decreased at 4 weeks, indicating improved oral health.

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2008·Applied and environmental microbiology·E Jiménez, L Fernández, A Maldonado, et al

Oral administration of Lactobacillus strains isolated from breast milk as an alternative for the treatment of infectious mastitis during lactation.

RCTn = 20Womens Health

RCT of 20 women with staphylococcal mastitis comparing probiotic treatment with Lactobacillus salivarius CECT5713 and Lactobacillus gasseri CECT5714 to a control. The probiotic group showed reduced staphylococcal counts and no clinical signs of mastitis by day 14, suggesting effectiveness in treating lactational infectious mastitis.

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2023·Frontiers in Cellular and Infection Microbiology·Qingqing Nie, Xuchun Wan, Hua Tao, et al

Multi-function screening of probiotics to improve oral health and evaluating their efficacy in a rat periodontitis model

Animal study

The study screened probiotics for oral health benefits, focusing on inhibiting pathogenic bacteria and biofilm formation. Bifidobacterium animalis ZK-77, Lactobacillus salivarius ZK-88, and Streptococcus salivarius ZK-102 showed antibacterial activity and improved oral health parameters in a rat periodontitis model. The probiotics/prebiotic combination also improved halitosis and teeth color in cats, with safety confirmed in mice.

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