Research

Chaga

57 peer-reviewed studies curated from PubMed and Semantic Scholar.

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Studies

Sorted by quality and recency

2025·International Journal of Molecular Sciences·Nidesha Randeni, Jinhai Luo, Yingzi Wu, et al

Elucidating the Anti-Diabetic Mechanisms of Mushroom Chaga (Inonotus obliquus) by Integrating LC-MS, Network Pharmacology, Molecular Docking, and Bioinformatics

StudyNutrition

The study investigates the anti-diabetic mechanisms of Chaga (Inonotus obliquus) using LC-MS, network pharmacology, molecular docking, and bioinformatics. It identifies 30 bioactive compounds and predicts 432 anti-diabetic targets, highlighting the PI3K/Akt signaling pathway as a key mechanism.

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2025·International Journal of Molecular Sciences·Yingzi Wu, Jiayi Liu, Jinhai Luo, et al

Molecular Mechanisms of Phytochemicals from Chaga Mushroom (Inonotus obliquus) Against Colorectal Cancer: Insights from Network Pharmacology, Molecular Docking, and Bioinformatics

StudyLongevity

The study explores the molecular mechanisms of phytochemicals from Chaga mushroom against colorectal cancer using network pharmacology, molecular docking, and bioinformatics. It identifies 26 bioactive compounds and 244 potential targets, suggesting Chaga mushroom phytochemicals may exert anticancer effects through multiple pathways.

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2025·Frontiers in pharmacology·Yueling Peng, Yaling Zhang, Rui Wang, et al

Corrigendum: Inonotus obliquus (chaga) ameliorates folic acid-induced renal fibrosis in mice: the crosstalk analysis among PT cells, macrophages and T cells based on single-cell sequencing.

Study

Corrigendum for a study on Inonotus obliquus (chaga) ameliorating folic acid-induced renal fibrosis in mice, analyzing crosstalk among PT cells, macrophages, and T cells using single-cell sequencing.

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2024·Journal of Fungi·Vesna Lazić, Anita Klaus, M. Kozarski, et al

The Effect of Green Extraction Technologies on the Chemical Composition of Medicinal Chaga Mushroom Extracts

Study

The study examined the effect of green extraction technologies on the chemical composition of Chaga mushroom extracts from Serbia and Mongolia. Subcritical water extraction (SWE) resulted in a higher yield and glucan content compared to other methods, while microwave-assisted extraction with 50% ethanol showed the highest yield of total phenols.

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2022·Journal of Food Bioactives·Han Peng, F. Shahidi

Qualitative analysis of secondary metabolites of chaga mushroom (Inonotus Obliquus): phenolics, fatty acids, and terpenoids

Study

The study conducted a qualitative analysis of secondary metabolites in chaga mushroom, identifying 111 phenolic, 63 fatty/aromatic acid, and 108 terpenoid constituents using HPLC-ToF-MS. It provided an updated compositional database of chaga, highlighting its potential as a food supplement with bioactive properties, though safety aspects like oxalate content require further investigation.

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2021·Antioxidants·Ibrahim M. Abu-Reidah, Amber L. Critch, Charles F. Manful, et al

Effects of pH and Temperature on Water under Pressurized Conditions in the Extraction of Nutraceuticals from Chaga (Inonotus obliquus) Mushroom

StudyNutrition Inflammation

The study evaluated a modified Swiss water process method using different pH and temperature conditions to extract bioactive compounds from Chaga mushroom. The process was effective in extracting phenolic compounds, vitamins, and minerals, with the highest phenolic content and antioxidant activity observed at pH 11.5 and 100 °C. The study suggests potential applications of Chaga extracts in the food and nutraceutical industries.

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2020·Proceedings of Universities. Applied Chemistry and Biotechnology·M. Sysoeva, L. N. Urazlina, V. R. Khabibrakhmanova, et al

Isolation of the Inonotus obliquus chaga mushroom strain and intensification of a culture growth during solid-phase cultivation

Study

The study isolated a new strain of Inonotus obliquus (chaga) and optimized its cultivation to enhance melanin production with high antioxidant properties. The new strain showed improved growth rates and antioxidant properties compared to natural chaga melanin.

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2020·Journal of food science·Hsinjung Chen, Bo-Kang Liou, Kuo-Chiang Hsu, et al

Implementation of food safety management systems that meets ISO 22000:2018 and HACCP: A case study of capsule biotechnology products of chaga mushroom.

Study

The study developed a food safety management system for a chaga mushroom biotechnology product manufacturer to meet ISO 22000:2018 and HACCP standards. It found a significant drop in coliform levels and a decline in defect rates for chaga products, indicating improved safety and quality levels.

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2026·Journal of microbiology and biotechnology·Junxiao Liu, Qun Zhang, Tianze Yang, et al

Chaga Mushroom () Attenuates DNCB-Induced Atopic Dermatitis by Modulating Oxidative Stress and Cytokine Expression.

Animal studySkin Hair Health Inflammation Immunity

The study evaluates the anti-atopic dermatitis activity of ethanol-extracted Chaga mushroom (E-CME) in BALB/c mice. E-CME treatment improved dermatitis scores, reduced mast cell infiltration, and modulated serum immunoglobulin levels, oxidative stress markers, and skin cytokine gene expression, suggesting its therapeutic potential for AD management.

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2025·International journal of molecular sciences·Soyoung Ban, Thuong Thi Do, Jang-Won Pyo, et al

INO10, a Chaga Mushroom Extract, Alleviates Alzheimer's Disease-Related Pathology and Cognitive Deficits in 3xTg-AD Mice.

Animal studyBrain Health Inflammation

The study evaluated the therapeutic potential of INO10, an inotodiol-rich chaga extract, in BV2 microglial cells and a 3xTg-AD mouse model. INO10 reduced pro-inflammatory cytokines in BV2 cells and improved spatial memory in 3xTg-AD mice, reducing Aβ deposition, tau phosphorylation, and neuroinflammation, suggesting its potential as a neuroprotective agent for Alzheimer's disease.

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2025·Bioscience, biotechnology, and biochemistry·Akio Nakane, Tatsuji Takahashi, Kenichi Ito, et al

The aqueous extracts of Inonotus obliquus (Chaga) augment host defense against Listeria monocytogenes infection.

Animal studyImmunity

The study investigates the effects of Chaga mushroom extracts on host defense against Listeria monocytogenes infection in mice. Chaga extracts enhanced cytokine production and inhibited bacterial proliferation in the liver, suggesting protective potential against infectious diseases like listeriosis.

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2025·Apoptosis : an international journal on programmed cell death·Aya Ezzat, Marwa A Ramadan, Jehane I Eid, et al

Bioactivity of mycosynthesized nanoparticles assists photothermal therapy of breast cancer cells.

In vitroWomens Health

The study explored the cytotoxic potential of gold nanoparticles synthesized using Chaga mushroom extract (AuCh-NPs) combined with LED irradiation on human breast cancer (MCF-7) cells. AuCh-NPs showed enhanced cytotoxicity under LED irradiation, with lower IC50 than citrate-capped gold nanoparticles, significant cell cycle arrest, apoptosis induction, and increased DNA damage, suggesting their potential as a targeted agent for photothermal therapy in breast cancer treatment.

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2025·Antioxidants (Basel, Switzerland)·Maria D'Elia, Carmen Marino, Rita Celano, et al

Impact of a Formulation Containing Chaga Extract, Coenzyme Q10, and Alpha-Lipoic Acid on Mitochondrial Dysfunction and Oxidative Stress: NMR Metabolomic Insights into Cellular Energy.

In vitroEnergy Brain Health

In vitro study on SH-SY5Y neuroblastoma cells treated with RP-25, a formulation containing Chaga extract, CoQ10, and alpha-lipoic acid. RP-25 increased metabolic pathways, enhanced cellular energy production, and showed antioxidant activity, mitigating mitochondrial dysfunction and oxidative stress.

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2025·Environmental toxicology and pharmacology·Zaved Ahmad, Pir Mohammad Ishfaq, Subodh Kumar Jain, et al

Mitigation of experimental hepatocellular Carcinoma by Inonotus obliquus Mushroom extract in association with oxidative stress and inflammation signaling.

Animal studyInflammation Immunity

The study investigated the effect of an aqueous extract of Inonotus obliquus (Chaga mushroom) on diethyl-nitrosamine/carbon tetrachloride-induced hepatocellular carcinoma in mice. IOAE administration significantly restored physiological and biochemical parameters altered by DEN/CCl, reduced tumor incidence, and ameliorated liver histopathology. The extract suppressed tumor incidence by reducing inflammation and inducing apoptosis.

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2024·Medical Research Archives·Sajeev Wagle, W. Thilakarathna, J. Lee, et al

Anti-Inflammatory Effect of Extracts of Inonotus obliquus and Microalgae

In vitroInflammation

In vitro study investigating the anti-inflammatory effects of extracts from Chaga mushroom and microalgae using a murine macrophage cell model. Chaga mushroom extract reduced proinflammatory biomarkers IL-6 and TNF-α, and suppressed cyclooxygenase-2 levels at high concentrations. Microalgae extract also suppressed IL-6 and TNF-α secretion.

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2024·Pharmaceuticals·A. Raal, H. Kaldmäe, Karin Kütt, et al

Chemical Content and Cytotoxic Activity on Various Cancer Cell Lines of Chaga (Inonotus obliquus) Growing on Betula pendula and Betula pubescens

In vitro

The study compared the chemical composition and cytotoxic activity of chaga mushroom extracts from Betula pendula and Betula pubescens on 31 human cancer cell lines. Chaga extracts showed moderate cytotoxic activity, with the strongest effects observed on HepG2 and CAL-62 cell lines. Chaga from B. pendula had somewhat stronger effects, attributed to higher inotodiol content.

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2024·Biomolecules·Junbiao Wang, Daniella Beghelli, Augusto Amici, et al

Chaga Mushroom Triterpenoids Inhibit Dihydrofolate Reductase and Act Synergistically with Conventional Therapies in Breast Cancer

In vitroImmunity

In vitro study of Chaga mushroom (Inonotus obliquus) water extract on breast cancer cell lines. Identified triterpenoids decreased cell viability, blocked cell cycle progression, and inhibited DHFR activity. Chaga components showed synergistic effects with cisplatin and trastuzumab, inhibiting HER2 and HER1 activation and displaying immunomodulatory effects.

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2024·Oxidative Medicine and Cellular Longevity·Sajeev Wagle, J. Lee, H. Rupasinghe

Synergistic Cytotoxicity of Extracts of Chaga Mushroom and Microalgae against Mammalian Cancer Cells In Vitro

In vitro

In vitro study investigating the cytotoxicity of extracts from Chaga mushroom (Inonotus obliquus) and microalgae against human and canine cancer cell lines. Ethanol-based and supercritical water extracts significantly inhibited cancer cell growth, with a synergistic effect observed when combining I. obliquus and microalgae extracts.

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2024·Polymers·Aditi Nagardeolekar, P. Dongre, Biljana M. Bujanovic

Lignin-Based Hydrogels for the Delivery of Bioactive Chaga Mushroom Extract

Study

The study synthesized lignin-based hydrogels as carriers for chaga mushroom extract and silver nanoparticles. The hydrogels were evaluated for their absorption and release behaviors, with those made from monocotyledons, wheat straw, and miscanthus found suitable.

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2024·Chinese journal of natural medicines·Yating Feng, Jing Liu, Le Gong, et al

Inonotus obliquus (Chaga) against HFD/STZ-induced glucolipid metabolism disorders and abnormal renal functions by regulating NOS-cGMP-PDE5 signaling pathway.

Animal studyNutrition Hormone Health Heart Health Inflammation

The study investigates the effects of Inonotus obliquus (Chaga) on glucolipid metabolism disorders and renal abnormalities in a rat model of type 2 diabetes mellitus induced by a high-fat diet and streptozotocin. Chaga improved metabolic dysfunctions and renal function markers, and modulated the NOS-cGMP-PDE5 signaling pathway, suggesting its potential in treating T2DM and diabetic nephropathy.

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2023·THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany)·Majdah Tuwaijri, S. Souad

Antibacterial activity of Chaga mushroom (Inonotus obliquus) extract against Staphylococcus aureus and Salmonella typhimurium: An In Vitro Study

In vitro

In vitro study testing the ethyl acetate extract of Chaga mushroom against Staphylococcus aureus and Salmonella typhimurium. The extract showed high antibacterial activity with inhibition zones of 25 mm and 28 mm, respectively, and MICs of 3.125 µg/ml for both pathogens. The study also explored the extract's effect on bacterial cell membrane permeability.

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2023·Journal of Biomolecular Structure and Dynamics·F. Amin, Abdo A. Elfiky, Aaya Nassar

In silico targeting of SARS-CoV-2 spike receptor-binding domain from different variants with chaga mushroom terpenoids

In vitro

In silico study examining the binding of chaga mushroom terpenoids to the spike receptor-binding domain (RBD) of various SARS-CoV-2 variants using molecular docking and dynamics modeling. The terpenoids showed better binding affinities to mutant RBDs compared to the wild-type, suggesting potential antiviral efficacy against different SARS-CoV-2 variants.

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2023·International Journal of Molecular Sciences·Jihyun Park, Thi Minh Nguyet Nguyen, H. Park, et al

Protective Effects of Lanostane Triterpenoids from Chaga Mushroom in Human Keratinocytes, HaCaT Cells, against Inflammatory and Oxidative Stresses

In vitroInflammation Skin Hair Health

In vitro study on the effects of inotodiol, a lanostane-type triterpenoid from Chaga mushrooms, on human keratinocyte cell line HaCaT cells. Inotodiol showed anti-inflammatory and anti-aging effects by suppressing pro-inflammatory cytokines and modulating collagen and hyaluronic acid synthesis.

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2023·International journal of medicinal mushrooms·Jun Lin, Yinfeng Lu, Hong-yu Shi, et al

Chaga Medicinal Mushroom, Inonotus obliquus (Agaricomycetes), Polysaccharides Alleviate Photoaging by Regulating Nrf2 Pathway and Autophagy.

In vitroSkin Hair Health

The study investigates the effects of Inonotus obliquus polysaccharides (IOP) on UVB-induced photoaging in human immortalized keratinocyte (HaCaT) cells. IOP was found to inhibit cell senescence and apoptosis, enhance antioxidant effects, and alleviate autophagy inhibition, suggesting its potential as a natural anti-photoaging ingredient for skin health.

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2023·International journal of biological sciences·Chang-Lim You, Sang-Jin Lee, Jinwoo Lee, et al

upregulates muscle regeneration and augments function through muscle oxidative metabolism.

Animal studyPhysical Performance Inflammation

The study investigates the effect of chaga (IO) extract on muscle regeneration and metabolism. IO treatment in C2C12 myoblasts increased myogenic differentiation and alleviated dexamethasone-induced myotube atrophy. In mice, IO improved regeneration of cardiotoxin-injured muscles and enhanced muscle oxidative metabolism, suggesting its potential in preventing muscle wasting.

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2023·Frontiers in Pharmacology·Amira Abugomaa, M. Elbadawy, Y. Ishihara, et al

Anti-cancer activity of Chaga mushroom (Inonotus obliquus) against dog bladder cancer organoids

In vitro

The study examined the anti-tumor potentials of Chaga mushroom extract against dog bladder cancer organoids. Chaga inhibited cell viability, arrested the cell cycle, and induced apoptosis in a concentration-dependent manner. It also decreased tumor growth in DBCO-derived xenografts in mice, suggesting its potential as a natural supplement to potentiate chemotherapy effects.

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2022·International journal of medicinal mushrooms·T. Teplyakova, O. Pyankov, Aleksandr S. Safatov, et al

Water Extract of the Chaga Medicinal Mushroom, Inonotus obliquus (Agaricomycetes), Inhibits SARS-CoV-2 Replication in Vero E6 and Vero Cell Culture Experiments.

In vitroImmunity

In vitro study of water extracts from the chaga mushroom (Inonotus obliquus) showing antiviral activity against SARS-CoV-2 in Vero E6 and Vero cell cultures. The extracts demonstrated low toxicity and inhibited viral replication with a 50% inhibitory concentration ranging from 0.75 to 11.6 μg/mL.

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2022·Molecules·Thi Minh Nguyet Nguyen, So-Young Ban, Kyu-been Park, et al

Evaluation of Toxicity and Efficacy of Inotodiol as an Anti-Inflammatory Agent Using Animal Model

Animal studyInflammation Immunity

The study evaluated the toxicity and efficacy of inotodiol, derived from chaga mushroom, as an anti-inflammatory agent in mice. Inotodiol administration showed no mortality or organ abnormalities and ameliorated food allergy symptoms, suggesting its potential as an immune modulator by inhibiting mast cell function.

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2022·Biochemistry (Moscow) Supplement. Series A, Membrane and Cell Biology·S. Efimova, P. D. Zlodeeva, E. V. Shekunov, et al

The Mechanisms of Lipid Vesicle Fusion Inhibition by Extracts of Chaga and Buckthorn Leaves

In vitro

The study evaluated the ability of extracts from grapefruit seeds, sea buckthorn leaves, and chaga to inhibit membrane fusion. Sea buckthorn leaves and chaga extracts significantly inhibited Ca2+-mediated fusion of lipid vesicles, while grapefruit seed extract did not. The inhibition was linked to flavonols quercetin and myricetin in sea buckthorn leaves.

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2022·Computers in Biology and Medicine·Wael M. Elshemey, Abdo A. Elfiky, Ibrahim M. Ibrahim, et al

Interference of Chaga mushroom terpenoids with the attachment of SARS-CoV-2; in silico perspective

In vitro

In-silico study using molecular docking and dynamics simulations to test Chaga mushroom terpenoids as potential therapeutics against the SARS-CoV-2 receptor GRP78. Results showed high affinities of Chaga mushroom terpenoids toward the GRP78 substrate-binding domain, suggesting potential for interfering with SARS-CoV-2 variants.

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2021·Phytochemistry·Rong-Wei Kou, Rui Han, Yu-qi Gao, et al

Anti-neuroinflammatory polyoxygenated lanostanoids from Chaga mushroom Inonotus obliquus.

In vitroInflammation

Chemical investigation of Chaga mushroom (Inonotus obliquus) identified seven new lanostane-type triterpenoids, which inhibited NO production in lipopolysaccharide-stimulated BV-2 microglial cells. Inonotusols I and L showed potent inhibitory effects on iNOS and NO production without cytotoxicity, suggesting potential anti-inflammatory effects.

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2021·International journal of medicinal mushrooms·Yizhou Xin, Ying Zhang, Xiaoli Zhang

Protective Effects of Chaga Medicinal Mushroom, Inonotus obliquus (Agaricomycetes), Extract on β-Amyloid-Induced Neurotoxicity in PC12 Cells and Aging Rats: In Vitro and In Vivo Studies.

In vitroBrain Health

The study evaluated the protective effects of Inonotus obliquus extracts on β-amyloid-induced neurotoxicity in PC12 cells and aging rats. IOE increased cell viability, decreased intracellular calcium levels, and reduced Aβ-mediated apoptosis in PC12 cells. In aging rats, IOE decreased amyloid precursor protein production and Aβ plaque levels in the hippocampus, associated with reduced IL-1β and TNF-α levels.

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2021·International journal of medicinal mushrooms·W. T. Basal, Abdo A. Elfiky, J. Eid

Chaga Medicinal Mushroom Inonotus obliquus (Agaricomycetes) Terpenoids May Interfere with SARS-CoV-2 Spike Protein Recognition of the Host Cell: A Molecular Docking Study.

In vitroImmunity

Molecular docking study investigating the antiviral potential of Inonotus obliquus terpenoids against COVID-19. The study found that terpenoid components, including betulinic acid and inonotusane C, can interact with the SARS-CoV-2 spike protein, potentially interfering with host cell recognition.

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2021·Food Science & Nutrition·J. Eid, Biswadeep Das, Majdah M. Al-Tuwaijri, et al

Targeting SARS‐CoV‐2 with Chaga mushroom: An in silico study toward developing a natural antiviral compound

In vitroImmunity

In silico study assessing the binding interaction of Chaga mushroom components with the SARS-CoV-2 receptor-binding domain. Beta glycan, betulinic acid, and galactomannan from Chaga showed strong binding interactions, suggesting potential as a natural antiviral supplement.

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2021·Journal of food biochemistry·Lin-Song Luo, Yu Wang, Lifeng Dai, et al

Triterpenoid acids from medicinal mushroom Inonotus obliquus (Chaga) alleviate hyperuricemia and inflammation in hyperuricemic mice: Possible inhibitory effects on xanthine oxidase activity.

Animal studyInflammation Joint Bone Health

The study explored the hypouricemic effect of triterpenoid acids from Inonotus obliquus (TAIO) in hyperuricemic mice, focusing on xanthine oxidase inhibitory mechanisms. TAIO showed strong inhibitory effects on XO activity, decreased uric acid levels, and ameliorated kidney damage and inflammation in hyperuricemic mice.

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2021·Frontiers in Immunology·P. A. M. A. Maza, J. Lee, Yong-Su Kim, et al

Inotodiol From Inonotus obliquus Chaga Mushroom Induces Atypical Maturation in Dendritic Cells

Animal studyImmunity

The study investigates the effects of inotodiol, a compound from Chaga mushroom, on the maturation of dendritic cells and T cell activation. Inotodiol increased the expression of maturation markers on dendritic cells and induced T cell proliferation and IL-2 production in mice, suggesting enhanced immune response.

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2021·Heliyon·J. Eid, M. Al-Tuwaijri, Swabhiman Mohanty, et al

Chaga mushroom (Inonotus obliquus) polysaccharides exhibit genoprotective effects in UVB-exposed embryonic zebrafish (Danio rerio) through coordinated expression of DNA repair genes

Animal studyImmunity Inflammation

The study investigated the genoprotective effects of Chaga mushroom polysaccharides (IOP) on UVB-exposed embryonic zebrafish. IOP treatment reduced DNA damage and improved the expression of DNA repair genes, mitigating UV-induced genotoxic effects and promoting normal development.

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2020·Applied Sciences·Tae In Kim, Jang-Gi Choi, J. Kim, et al

Blocking Effect of Chaga Mushroom (Inonotus oliquus) Extract for Immune Checkpoint CTLA-4/CD80 Interaction

In vitroImmunity

The study examined the blocking effect of Chaga mushroom extracts on the CTLA-4/CD80 interaction using cell-based assays. Ethanol and water extracts of Chaga mushroom showed inhibitory activities, suggesting potential as a small-molecule inhibitor for immune modulation.

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2020·International immunopharmacology·Thi Minh Nguyet Nguyen, H. Le, B. Le, et al

Anti-allergic effect of inotodiol, a lanostane triterpenoid from Chaga mushroom, via selective inhibition of mast cell function.

Animal studyImmunity Inflammation

The study investigates the anti-allergic effects of inotodiol, a compound from Chaga mushroom, in a mouse model of food allergy. Inotodiol significantly improved allergic symptoms and inflammatory lesions in the small intestine by selectively inhibiting mast cell function, making it a promising therapeutic candidate for food allergy.

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2020·Nutrients·Jun Fang, Shanghui Gao, Rayhanul Islam, et al

Extracts of Phellinus linteus, Bamboo (Sasa senanensis) Leaf and Chaga Mushroom (Inonotus obliquus) Exhibit Antitumor Activity through Activating Innate Immunity

Animal studyImmunity

The study investigated the antitumor activity of MeshimaMax, a combination of extracts from Phellinus linteus, bamboo leaf, and Chaga mushroom, in various solid tumor models. MeshimaMax significantly inhibited tumor growth in mouse sarcoma S180 and suppressed tumor occurrence in mouse colon and rat breast cancer models. It also enhanced the therapeutic effect when combined with a chemotherapeutic drug and activated macrophage phagocytosis in vitro.

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2020·International journal of medicinal mushrooms·Hui Liao, D. Jia, Xiaoyun Zhao, et al

Effects of Chaga Medicinal Mushroom Inonotus obliquus (Agaricomycetes) Extracts on NOS-cGMP-PDE5 Pathway in Rat Penile Smooth Muscle Cells.

In vitroSexual Health

The study investigated the effects of chaga mushroom extracts on the NOS-cGMP-PDE5 pathway in rat penile smooth muscle cells. IO2 extract, with higher polysaccharide content, showed greater antioxidant activity and increased eNOS and iNOS expression, suggesting potential benefits for erectile dysfunction treatment.

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2020·Scientific Reports·J. Eid, Biswadeep Das

Molecular insights and cell cycle assessment upon exposure to Chaga (Inonotus obliquus) mushroom polysaccharides in zebrafish (Danio rerio)

Animal studyInflammation

The study assessed the in vivo developmental effects of Chaga mushroom polysaccharides in zebrafish, focusing on cell cycle and apoptosis. Chaga polysaccharides reduced intracellular ROS and assisted in normal zebrafish development, with most embryos remaining healthy even at high concentrations. The polysaccharides also moderately arrested cells at the G1 stage, suggesting potential for cancer studies.

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2024·International journal of medicinal mushrooms·Olga N Antosyuk, Victoria V Kostenko, Aleksandr A Ermoshin, et al

Extracts from Four Species of Xylotrophic Basidiomycetes Growing in the Middle Urals (Russia) Revealed Cytotoxic Effects on Model Object Drosophila melanogaster.

In vitro

The study assessed the cytotoxic and genotoxic effects of aqueous-alcoholic extracts from four species of wood-decaying fungi on Drosophila melanogaster. Extracts from I. obliquus and G. applanatum caused the greatest DNA damage, while F. fomentarius showed the lowest DNA damage and reduced GstD1 gene expression.

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2024·Heliyon·Emma Camilleri, R. Blundell, Bikash Baral, et al

A brief overview of the medicinal and nutraceutical importance of Inonotus obliquus (chaga) mushrooms

ReviewImmunity Inflammation Longevity

This literature review explores Chaga mushrooms (Inonotus obliquus), focusing on their phytochemical composition and health-promoting attributes. It highlights Chaga's anticancer, antioxidant, anti-diabetic, anti-inflammatory, antimicrobial, and immunomodulating properties, emphasizing its potential role in diverse health benefits.

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2023·Frontiers in Pharmacology·Eric Fordjour, Charles F. Manful, Rabia Javed, et al

Chaga mushroom: a super-fungus with countless facets and untapped potential

Review

Narrative review of Chaga mushroom (Inonotus obliquus) exploring its ethnobotany, mycochemistry, pharmacology, and traditional therapeutic uses. The review highlights its potential as an antioxidant, anti-inflammatory, antiviral, and antitumor agent, and discusses its applications in pharmaceuticals, food, cosmetics, and agriculture.

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2023·Mycology·Phoebe Tee Yon Ern, T. Quan, Fung Shin Yee, et al

Therapeutic properties of Inonotus obliquus (Chaga mushroom): A review

Review

This review explores the therapeutic properties of Inonotus obliquus (Chaga mushroom), highlighting its historical use in folk medicine and modern scientific evidence supporting its anti-inflammatory, antioxidant, anticancer, anti-diabetic, anti-obesity, hepatoprotective, renoprotective, anti-fatigue, antibacterial, and antiviral activities. The review discusses bioactive compounds responsible for these effects and their mechanisms of action.

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2021·Polymers·Yangpeng Lu, Yanan Jia, Zihan Xue, et al

Recent Developments in Inonotus obliquus (Chaga mushroom) Polysaccharides: Isolation, Structural Characteristics, Biological Activities and Application

Review

This review summarizes recent advancements in the extraction, purification, structural characteristics, and biological activities of Inonotus obliquus polysaccharides (IOPS). It highlights the antitumor, antioxidant, antiviral, hypoglycemic, and hypolipidemic activities of IOPS, and discusses its potential applications in treating cancers and type 2 diabetes.

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2020·Journal of Food Bioactives·Han Peng, F. Shahidi

Bioactive Compounds and Bioactive Properties of Chaga (Inonotus obliquus) Mushroom: A Review

Review

This review discusses the bioactive compounds and bioactive properties of Chaga (Inonotus obliquus) mushroom, including its antioxidant, anti-inflammatory, anti-tumor, immunomodulatory, and other activities. It also addresses toxicity concerns and risk assessments related to its use as a medicinal/nutraceutical product.

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2020·Journal of Korean Medical Science·Sua Lee, Hwa Young Lee, Yohan Park, et al

Development of End Stage Renal Disease after Long-Term Ingestion of Chaga Mushroom: Case Report and Review of Literature

Case report

Case report of a 49-year-old Korean man developing end-stage renal disease after long-term ingestion of Chaga mushroom. Kidney biopsy showed chronic tubulointerstitial nephritis with oxalate crystal deposits, and the Chaga mushroom was found to have high oxalate content, suggesting a link to oxalate nephropathy.

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2019·International journal of medicinal mushrooms·Xin Xin, Jing Qu, V. Veeraraghavan, et al

Assessment of the Gastroprotective Effect of the Chaga Medicinal Mushroom, Inonotus obliquus (Agaricomycetes), Against the Gastric Mucosal Ulceration Induced by Ethanol in Experimental Rats.

Animal study

The study assessed the gastroprotective effect of the chaga medicinal mushroom (Inonotus obliquus) against ethanol-induced gastric mucosal ulceration in rats. The ethanolic extract of I. obliquus exhibited effective antiulcer activity without causing toxicity, supporting its traditional use for treating ailments.

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2019·Journal of Cosmetics, Dermatological Sciences and Applications·Mohamed Softa, G. Percoco, E. Lati, et al

Birch Sap (Betula alba) and Chaga Mushroom (Inonotus obliquus) Extracts Show Anti-Oxidant, Anti-Inflammatory and DNA Protection/Repair Activity In Vitro

In vitroSkin Hair Health Inflammation

In vitro study assessing the UV protection activity of birch sap and chaga mushroom extracts on skin cells. The extracts showed strong anti-oxidant, anti-inflammatory, and DNA protection/repair activities, suggesting potential for anti-photo-aging cosmetic products.

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2019·International journal of medicinal mushrooms·Zhiguo Li, J. Mei, Liping Jiang, et al

Chaga Medicinal Mushroom, Inonotus obliquus (Agaricomycetes) Polysaccharides Suppress Tacrine-induced Apoptosis by ROS-scavenging and Mitochondrial Pathway in HepG2 Cells.

In vitro

In vitro study investigating the protective effect of polysaccharides from Inonotus obliquus (chaga mushroom) on tacrine-induced apoptosis in HepG2 cells. The study found that I. obliquus polysaccharides reduced apoptosis by inhibiting ROS generation and mitochondrial dysfunction, suggesting a potential protective mechanism against tacrine-induced hepatotoxicity.

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2018·Journal of ethnopharmacology·J. Baek, H. Roh, Kwan-Hyuck Baek, et al

Bioactivity-based analysis and chemical characterization of cytotoxic constituents from Chaga mushroom (Inonotus obliquus) that induce apoptosis in human lung adenocarcinoma cells.

In vitroLongevity

The study evaluated the cytotoxic activity of Chaga mushroom (Inonotus obliquus) in four human lung adenocarcinoma cell lines. Bioactivity-guided fractionation led to the isolation of eight triterpenoids, with compounds 1, 6, and 7 showing potent cytotoxic activity mediated by apoptosis and caspase-3 activation.

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2015·Nutrition Research and Practice·H. Lee, E. Kim, S. H. Kim

Ethanol extract of Innotus obliquus (Chaga mushroom) induces G1 cell cycle arrest in HT-29 human colon cancer cells

In vitro

In vitro study examining the effects of ethanol extract of Inonotus obliquus (Chaga mushroom) on HT-29 human colon cancer cells. The extract induced G1 cell cycle arrest, reduced viable cell numbers, and altered expression of cell cycle-related proteins, suggesting potential anti-cancer properties.

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2008·World journal of gastroenterology·M. Youn, Jin-Kyung Kim, Seong-Yeol Park, et al

Chaga mushroom (Inonotus obliquus) induces G0/G1 arrest and apoptosis in human hepatoma HepG2 cells.

In vitro

In vitro study investigating the anti-proliferative and apoptotic effects of Chaga mushroom water extract on human hepatoma cell lines HepG2 and Hep3B. Chaga extract induced G0/G1-phase arrest and apoptotic cell death, with HepG2 cells showing more sensitivity.

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2022·Medicine·Ohyun Kwon, Yaerim Kim, J. Paek, et al

Chaga mushroom-induced oxalate nephropathy that clinically manifested as nephrotic syndrome

Case reportn = 1

Case report of a 69-year-old man who developed acute kidney injury with nephrotic syndrome after ingesting Chaga mushroom powder and vitamin C. Pathological findings indicated acute oxalate nephropathy and minimal change disease. The patient's kidney function recovered after hemodialysis and high-dose steroid treatment.

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2019·Journal of microbiology and biotechnology·H. Na, Yuna Park, Min-Ah Kim, et al

Secondary Fermented Extract of Chaga-Cheonggukjang Attenuates the Effects of Obesity and Suppresses Inflammatory Response in the Liver and Spleen of High-Fat Diet-Induced Obese Mice.

Animal studyn = 40Inflammation Physique

Animal study on the effects of a secondary fermented extract of Chaga-Cheonggukjang (FCC) on high-fat diet-induced obese mice. FCC supplementation reduced body weight and epididymal fat pad weight, suppressed serum triglyceride levels, increased serum HDL-C levels, and attenuated inflammatory responses in the liver and spleen.

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