Research
Black Seed Oil
37 peer-reviewed studies curated from PubMed and Semantic Scholar.
Studies
Sorted by quality and recency
A Comparative Study on Alvogyl and a Mixture of Black Seed Oil and Powder for Alveolar Osteitis: A Randomized Double-Blind Controlled Clinical Trial.
RCT comparing the effects of Alvogyl and a mixture of black seed oil and powder in the treatment of alveolar osteitis (dry sockets) in 60 patients. The black seed oil mixture showed statistically significant pain relief and required fewer dressings compared to the Alvogyl group.
Amelioration of Endothelial Dysfunction and Inflammation in Type 2 Diabetic Patients after Black Seed Oil Supplementation.
Prospective, randomized, placebo-controlled, double-blinded study evaluating the impact of 1800 mg/day black seed oil supplementation on endothelial dysfunction and inflammation in type 2 diabetic patients over 12 weeks. Significant improvements were observed in hemoglobin A1C, total cholesterol, triglycerides, hsCRP, ICAM-1, and diabetes control domain scores in the intervention group compared to the control group.
Effects ofon Type-2 Diabetes Mellitus: A Systematic Review.
Systematic review of the effects of black seed oil on glucose and insulin profile status in humans. The review found significant improvements in laboratory parameters of hyperglycemia and diabetes control, including reductions in fasting blood glucose, postprandial blood glucose, glycated hemoglobin, and insulin resistance, with an increase in serum insulin.
Black seed (Nigella sativa) as an adjuvant therapy in the treatment of patients with rheumatoid arthritis Clinical trial
Clinical trial investigating the effects of 1000 mg black seed oil daily as adjuvant therapy in 25 RA patients taking DMARDs for eight weeks. Significant improvements were observed in DAS28 score and VAS for pain, with decreased TNF-α levels and WBC count, indicating reduced inflammation.
Protective role of black seed oil in doxorubicin-induced cardiac toxicity in children with acute lymphoblastic leukemia.
RCT assessing the protective role of black seed oil against doxorubicin-induced cardiotoxicity in 40 children with acute lymphoblastic leukemia. Black seed oil improved systolic cardiac functions compared to placebo, suggesting a cardioprotective effect.
Layer-by-Layer Engineering of Black Seed Oil Based SNEDDSs (BSO-SNEDDSs): Optimizing Chemical Stability and Bioavailability in Ramipril Formulations
The study developed ramipril-loaded self-nanoemulsifying formulations incorporating black seed oil for enhanced chemical stability and bioavailability. In vitro and in vivo analyses showed that liquid SNEDDSs had higher bioavailability compared to pure ramipril, though not statistically significant. Multilayer solid SNEDDSs showed lower ramipril exposure, indicating a need for further refinement.
Evaluation of extraction and entrapment efficiency of black seed oil-containing emulsion as a delivery system for thymoquinone
The study aimed to validate a GC-FID method for quantifying thymoquinone (TQ) in black seed oil (BSO) and BSO-containing emulsion. It evaluated TQ extraction and entrapment efficiency, finding a 64.83% entrapment efficiency in the emulsion using a density-changing centrifugation method.
Effect of black seed oil, honey, whey protein concentrate and their interaction on antioxidant activity, elastic modulus and creaming index of O/W emulsions
The study investigated the effects of black seed oil, honey, and whey protein concentrate on the antioxidant activity, elastic modulus, and creaming index of oil-in-water emulsions. Using response surface methodology, it was found that honey and whey protein concentrate significantly affected antioxidant activity, while the combination of black seed oil and honey showed synergistic effects. Optimized levels of the substances were proposed for desired emulsion properties.
Development Liquid and Solid Self Microemulsifying Drug Delivery System (L-SMEDDS and S-SMEDDS) Containing Black Seed Oil (Nigella sativa L.)
The study aimed to develop and characterize liquid and solid self-microemulsifying drug delivery systems (L-SMEDDS and S-SMEDDS) containing black seed oil. The formulations were evaluated for various physical characteristics and stability, with the best formula showing good emulsification time, dispersibility, and stability.
Development of self nanoemulsifying drug delivery system for black seed oil (Nigella sativa L.)
The study aimed to develop black seed oil into a self nanoemulsifying drug delivery system (SNEDDS) to enhance its oral bioavailability. The optimized SNEDDS formulation used tween 80 as a surfactant and PEG 400 as a cosurfactant, achieving good physical characteristics and stability.
Therapeutic value of black seed oil in methotrexate hepatotoxicity in Egyptian children with acute lymphoblastic leukemia.
RCT conducted on 40 children with acute lymphoblastic leukemia to assess the therapeutic value of Black seed oil in methotrexate-induced hepatotoxicity. Black seed oil decreased MTX hepatotoxicity and improved survival in children, suggesting its potential as an adjuvant therapy.
The clinical outcome of adjuvant therapy with black seed oil on intractable paediatric seizures: a pilot study.
A prospective, randomised, single-blinded, controlled, crossover pilot study evaluated the effect of black seed oil as an add-on treatment to antiepileptic drugs in 30 children with intractable epilepsy. The study found no significant difference in seizure frequency, severity, or oxidative stress markers after black seed oil administration compared to placebo.
Black seed oil boosts antidiabetic activity of glibenclamide: development of solidified self nanoemulsifying drug delivery system and evaluation in Streptozotocin-Induced diabetic rat model
The study developed a solidified self nano-emulsifying drug delivery system (SNEDDS) containing black seed oil and glibenclamide to enhance antidiabetic activity. In a streptozotocin-induced diabetic rat model, the SNEDDS formulation significantly reduced blood glucose levels compared to pure glibenclamide, demonstrating superior efficacy.
Black seed oil nanoemulsion containing albendazole against protoscoleces of Echinococcus granulosus: An in vivo study on C57BL/6 mice
The study explored a nanoemulsion of black seed oil to enhance the therapeutic efficacy of albendazole in mice with hydatid cysts. The nanoemulsion containing albendazole showed reduced cytotoxicity and a significant decrease in size and number of cysts compared to free albendazole or nanoemulsion without the drug. The nanoemulsion also resulted in 100% survival after 60 days.
Evaluating the taste-masking ability and sensory attributes of alginate-encapsulated black seed oil
The study evaluated the sensory attributes of alginate-encapsulated black seed oil in 12 healthy volunteers. Encapsulation significantly improved the taste and aftertaste of black seed oil, with vanilla and orange flavors being most effective in enhancing palatability. The study highlights the potential of alginate encapsulation for taste masking.
SLN and chitosan nano-delivery systems for antibacterial effect of black seed (Nigella sativa) oil against S. aureus
The study evaluated the antibacterial effects of Nigella Sativa oil (NSO) against Staphylococcus aureus using nano-carriers (NS-SLN and NS-chitosan nanoparticles). The results showed significantly stronger antibacterial effects of NSO when loaded into chitosan nanoparticles, suggesting its potential as a nano-delivery system for antimicrobial agents.
Hydroxyapatite/Chondroitin Sulphate Nanocomposite Infused with Black Seed Oil for Bone Tissue Applications: Physicochemical Characterizations and In Vitro Screening
The study investigates the impact of black seed extract on a chondroitin sulfate-based hydroxyapatite nanocomposite for bone tissue regeneration. The HCSK3 nanocomposite with black seed extract showed strong molecular interactions, good thermal stability, and enhanced cell viability, suggesting its potential as a bone implant.
Synthesis of Black Seed Oil-loaded-, Alginate-based, pH-sensitive Beads Using Electrospraying Technique.
The study synthesized pH-sensitive alginate beads to deliver black seed oil (BSO) into the colon using electrospraying technology. The beads demonstrated high encapsulation efficiency and controlled release properties, with more than 90% release at pH 6.8, suggesting potential benefits for gut health and inflammation reduction.
Effect of ozonation on the phytochemicals of black seed oil and its anti-microbial, anti-oxidant, anti-inflammatory, and anti-neoplastic activities in vitro
In vitro study assessing the effects of ozonized black seed oil on its chemical profile and biological activities. Ozonized black seed oil showed improved antimicrobial activity, antioxidant capacity, anti-inflammatory impact, and antitumor capacity towards HCT cells compared to crude oil.
Development of a Multifunctional Oral Dosage Form via Integration of Solid Dispersion Technology with a Black Seed Oil-Based Self-Nanoemulsifying Drug Delivery System
The study developed a multifunctional oral dosage form integrating solid dispersion technology with a black seed oil-based self-nanoemulsifying drug delivery system to enhance lansoprazole delivery and therapeutic effects. The formulations were characterized for particle size, stability, and dissolution efficiency, with lyophilized solid dispersions showing improved stability and dissolution efficiency.
Effect of Encapsulation Material on Lipid Bioaccessibility and Oxidation during In Vitro Digestion of Black Seed Oil
The study investigated the effect of encapsulation materials, starch and gelatin, on lipid hydrolysis, bioaccessibility, and oxidation of black seed oil during in vitro digestion. Starch increased lipid hydrolysis during gastric digestion, while no differences were observed in intestinal digestates. Starch and gelatin encapsulation led to a significant rise in free oxylipins after intestinal digestion, with gelatin being more effective in minimizing oxidation.
Black seed oil impact on authentic kefir microbiota
The study investigated the impact of different concentrations of black seed oil on kefir microorganisms. Results showed that 5% black seed oil significantly inhibited kefir microorganisms, while lower concentrations did not show significant effects.
Promoting action of vitamin E and black seed oil on reproductive hormones and organ histoarchitecture of Swiss albino mice
The study investigated the effects of vitamin E and black seed oil on reproductive performance in Swiss albino mice. Results showed increased testosterone levels and seminiferous tubule diameter in male mice, and an increased number of ovarian follicles in female mice, suggesting potential benefits for reproductive functions.
Anti-nociceptive effect of black seed oil on an animal model of chronic constriction injury
The study investigated the antihyperalgesic effect of black seed oil (BSO) in an animal model of neuropathic pain. Chronic administration of BSO significantly attenuated heat hyperalgesia and mechanical allodynia in rats with chronic constriction injury, suggesting potential use in managing hyperalgesia and allodynia.
Combined Ramipril and Black Seed Oil Dosage Forms Using Bioactive Self-Nanoemulsifying Drug Delivery Systems (BIO-SNEDDSs)
The study developed a combined liquid SNEDDS formulation containing ramipril and black seed oil (as a source of thymoquinone) to improve oral bioavailability. The optimized formulation showed enhanced self-emulsification efficiency and good release of ramipril and thymoquinone in in-vitro dissolution studies.
Amelioration effect of black seed oil against high-fat diet-induced obesity in rats through Nrf2/HO-1 pathway.
The study investigated the effects of black seed oil on high-fat diet-induced obesity in rats. Black seed oil significantly decreased insulin resistance, body weight, and normalized lipid profiles. It also reduced oxidative stress and improved liver function through the Nrf2/HO-1 pathway.
Self-emulsifying drug delivery system of black seed oil with improved hypotriglyceridemic effect and enhanced hepatoprotective function
The study developed a self-emulsifying drug delivery system (SEDDS) for Black Seed Oil (BSO) to improve its hypotriglyceridemic and hepatoprotective effects. In a CCl4-treated rat model, SEDDS-BSO significantly reduced liver enzyme levels, indicating reduced hepatotoxicity. Additionally, it showed a potent hypotriglyceridemic effect in rats with induced hypertriglyceridemia compared to BSO alone.
Encapsulation of Black Seed Oil in Alginate Beads as a pH-Sensitive Carrier for Intestine-Targeted Drug Delivery: In Vitro, In Vivo and Ex Vivo Study
The study developed alginate-black seed oil beads as a controlled release system for drug delivery in the gastrointestinal tract. The beads showed high encapsulation efficiency and significant drug release in simulated gastrointestinal fluid, suggesting potential for increased bioavailability and targeted delivery in conditions like irritable bowel syndrome.
Black seed oil induced acute liver failure: a case report and literature review
Case report of an 82-year-old female with diabetes, hypertension, and chronic kidney disease who developed acute liver failure after using black seed oil. Despite treatment in the intensive care unit, the patient died on the 9th day of hospitalization. The report highlights potential toxic effects of herbal products like black seed oil, especially in patients on renal replacement therapy.
The Protecting Role of Black Seed Oil and Its Nano-Formulation in LPS-Induced Acute Kidney Injury in Mice: Evaluation of Oxidative Stress, Biochemical & Molecular Parameters
The study investigated the protective effects of black seed oil (BSO) and its nano-formulation on LPS-induced acute kidney injury in mice. BSO and its nano-formulation showed anti-inflammatory effects comparable to or better than indomethacin, significantly reducing oxidative stress and inflammatory markers, and protecting against pathological alterations in kidney tissues.
Black seed oil reverses chronic antibiotic-mediated depression and social behaviour deficits via modulation of hypothalamic mitochondrial-dependent markers and insulin expression
The study assessed the impact of black seed oil on depression, sociability, redox imbalance, mitochondrial markers, and insulin expression in mice exposed to chronic ampicillin. Black seed oil ameliorated depressive-like behaviors, social interaction deficits, and mitochondrial disruptions caused by antibiotic exposure.
In vivo assessment of black seed oil single dose on prednisolone pharmacokinetics.
The study investigated the effect of a single dose of black seed oil on prednisolone pharmacokinetics in rats. Results showed that black seed oil decreased prednisolone Cmax and AUC0-last, indicating a herb-drug interaction, though p-gp inhibition could not be concluded.
Hepato-Renal Dysfunctions Induced by Gold Nanoparticles and Preservative Efficacy of Black Seed Oil
Animal study investigating the toxic effects of gold nanoparticles on liver and kidney functions in rats and the protective efficacy of black seed oil. Rats treated with gold nanoparticles showed increased levels of hepato-renal biomarkers, while co-administration of black seed oil reduced these disturbances, indicating a protective effect.
Development of 3D-Printed, Liquisolid and Directly Compressed Glimepiride Tablets, Loaded with Black Seed Oil Self-Nanoemulsifying Drug Delivery System: In Vitro and In Vivo Characterization
The study developed glimepiride tablets using black seed oil-based self-nanoemulsifying drug delivery system (SNEDDS) and evaluated their pharmacokinetics in Male Wistar rats. The SNEDDS formulation improved glimepiride solubility and bioavailability, with liquisolid and 3D-printed tablets showing enhanced pharmacokinetic parameters compared to commercial products.
Growth performance, hematological parameters and lipid profile of mice treated with black seed oil and vitamin-E
Animal study on the effects of black seed oil and vitamin E on growth performance and blood biochemistry in mice. Groups treated with black seed oil, vitamin E, or both showed significant decreases in body weight and changes in hemoglobin and erythrocyte count compared to controls. Serum cholesterol and triglyceride levels decreased, while HDL increased significantly in treated groups.
Protective Effect of Combination Commercial Black Seed Oil (Nigella sativa) and Honey Against Cisplatin-Induced Hepatotoxicity in Rats
Animal study evaluating the protective effect of black seed oil and honey against cisplatin-induced hepatotoxicity in rats. The combination of black seed oil and honey reduced malondialdehyde levels, indicating a protective effect on liver tissue, although the combination showed an antagonism effect.
Structural Characterization, Antimicrobial Activity, and In Vitro Cytotoxicity Effect of Black Seed Oil
The study investigates the structural components of black seed oil (BSO) and its antimicrobial and cytotoxic effects. BSO showed strong bacterial inhibitory effects, particularly against Bacillus subtilis and MRSA, without cytotoxicity towards human PBMCs. Thymoquinone was identified as a major compound responsible for the antibacterial activity.